Lrp5 (NM_008513) Mouse Untagged Clone

CAT#: MC203730

Lrp5 (untagged) - Mouse low density lipoprotein receptor-related protein 5 (Lrp5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lrp5
• Synonyms: BMND1; HBM; LR3; LRP7; mKIAA4142; OPPG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC203730 representing NM_008513.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCG
  CGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC
  ATGGAAACGGCGCCGACCCGGGCCCCTCCGCCGCCGCCGCCGCCGCTGCTGCTGCTGGTGCTGTACTGC
  AGCTTGGTCCCCGCCGCGGCCTCACCGCTCCTGTTGTTTGCCAACCGCCGGGATGTGCGGCTAGTGGAT
  GCCGGCGGAGTGAAGCTGGAGTCCACCATTGTGGCCAGTGGCCTGGAGGATGCAGCTGCTGTAGACTTC
  CAGTTCTCCAAGGGTGCTGTGTACTGGACAGATGTGAGCGAGGAGGCCATCAAACAGACCTACCTGAAC
  CAGACTGGAGCTGCTGCACAGAACATTGTCATCTCGGGCCTCGTGTCACCTGATGGCCTGGCCTGTGAC
  TGGGTTGGCAAGAAGCTGTACTGGACGGACTCCGAGACCAACCGCATTGAGGTTGCCAACCTCAATGGG
  ACGTCCCGTAAGGTTCTCTTCTGGCAGGACCTGGACCAGCCAAGGGCCATTGCCCTGGATCCTGCACAT
  GGGTACATGTACTGGACTGACTGGGGGGAAGCACCCCGGATCGAGCGGGCAGGGATGGATGGCAGTACC
  CGGAAGATCATTGTAGACTCCGACATTTACTGGCCCAATGGGCTGACCATCGACCTGGAGGAACAGAAG
  CTGTACTGGGCCGATGCCAAGCTCAGCTTCATCCACCGTGCCAACCTGGACGGCTCCTTCCGGCAGAAG
  GTGGTGGAGGGCAGCCTCACTCACCCTTTTGCCCTGACACTCTCTGGGGACACACTCTACTGGACAGAC
  TGGCAGACCCGCTCCATCCACGCCTGCAACAAGTGGACAGGGGAGCAGAGGAAGGAGATCCTTAGTGCT
  CTGTACTCACCCATGGACATCCAAGTGCTGAGCCAGGAGCGGCAGCCTCCCTTCCACACACCATGCGAG
  GAGGACAACGGTGGCTGTTCCCACCTGTGCCTGCTGTCCCCGAGGGAGCCTTTCTACTCCTGTGCCTGC
  CCCACTGGTGTGCAGTTGCAGGACAATGGCAAGACGTGCAAGACAGGGGCTGAGGAAGTGCTGCTGCTG
  GCTCGGAGGACAGACCTGAGGAGGATCTCTCTGGACACCCCTGACTTCACAGACATAGTGCTGCAGGTG
  GGCGACATCCGGCATGCCATTGCCATTGACTACGATCCCCTGGAGGGCTACGTGTACTGGACCGACGAT
  GAGGTGCGGGCTATCCGCAGGGCGTACCTAGATGGCTCAGGTGCGCAGACACTTGTGAACACTGAGATC
  AATGACCCCGATGGCATTGCTGTGGACTGGGTCGCCCGGAACCTCTACTGGACAGATACAGGCACTGAC
  AGAATTGAGGTGACTCGCCTCAACGGCACCTCCCGAAAGATCCTGGTATCTGAGGACCTGGACGAACCG
  CGAGCCATTGTGTTGCACCCTGTGATGGGCCTCATGTACTGGACAGACTGGGGGGAGAACCCCAAAATC
  GAATGCGCCAACCTAGATGGGAGAGATCGGCATGTCCTGGTGAACACCTCCCTTGGGTGGCCCAACGGA
  CTGGCCCTGGACCTGCAGGAGGGCAAGCTGTACTGGGGGGATGCCAAAACTGATAAAATCGAGGTGATC
  AACATAGATGGGACAAAGCGGAAGACCCTGCTTGAGGACAAGCTCCCACATATTTTTGGGTTCACACTG
  CTGGGGGACTTCATCTACTGGACCGACTGGCAGAGACGCAGTATTGAAAGGGTCCACAAGGTCAAGGCC
  AGCCGGGATGTCATCATTGATCAACTCCCCGACCTGATGGGACTCAAAGCTGTGAATGTGGCCAAGGTT
  GTCGGAACCAACCCATGTGCGGATGGAAATGGAGGGTGCAGCCATCTGTGCTTCTTCACCCCACGTGCC
  ACCAAGTGTGGCTGCCCCATTGGCCTGGAGCTGTTGAGTGACATGAAGACCTGCATAATCCCCGAGGCC
  TTCCTGGTATTCACCAGCAGAGCCACCATCCACAGGATCTCCCTGGAGACTAACAACAACGATGTGGCT
  ATCCCACTCACGGGTGTCAAAGAGGCCTCTGCACTGGACTTTGATGTGTCCAACAATCACATCTACTGG
  ACTGATGTCAGCCTCAAGACGATCAGCCGAGCCTTCATGAATGGGAGCTCAGTGGAGCACGTGATTGAG
  TTTGGCCTCGACTACCCTGAAGGAATGGCTGTGGACTGGATGGGCAAGAACCTCTATTGGGCGGACACA
  GGGACCAACAGGATTGAGGTGGCCCGGCTGGATGGGCAGTTCCGGCAGGTGCTTGTGTGGAGAGACCTT
  GACAACCCCAGGTCTCTGGCTCTGGATCCTACTAAAGGCTACATCTACTGGACTGAGTGGGGTGGCAAG
  CCAAGGATTGTGCGGGCCTTCATGGATGGGACCAATTGTATGACACTGGTAGACAAGGTGGGCCGGGCC
  AACGACCTCACCATTGATTATGCCGACCAGCGACTGTACTGGACTGACCTGGACACCAACATGATTGAG
  TCTTCCAACATGCTGGGTCAGGAGCGCATGGTGATAGCTGACGATCTGCCCTACCCGTTTGGCCTGACT
  CAATATAGCGATTACATCTACTGGACTGACTGGAACCTGCATAGCATTGAACGGGCGGACAAGACCAGT
  GGGCGGAACCGCACCCTCATCCAGGGTCACCTGGACTTCGTCATGGACATCCTGGTGTTCCACTCCTCC
  CGTCAGGATGGCCTCAACGACTGCGTGCACAGCAATGGCCAGTGTGGGCAGCTGTGCCTCGCCATCCCC
  GGAGGCCACCGCTGTGGCTGTGCTTCACACTACACGCTGGACCCCAGCAGCCGCAACTGCAGCCCGCCC
  TCCACCTTCTTGCTGTTCAGCCAGAAATTTGCCATCAGCCGGATGATCCCCGATGACCAGCTCAGCCCG
  GACCTTGTCCTACCCCTTCATGGGCTGAGGAACGTCAAAGCCATCAACTATGACCCGCTGGACAAGTTC
  ATCTACTGGGTGGACGGGCGCCAGAACATCAAGAGGGCCAAGGACGACGGTACCCAGCCCTCCATGCTG
  ACCTCTCCCAGCCAAAGCCTGAGCCCAGACAGACAGCCACACGACCTCAGCATTGACATCTACAGCCGG
  ACACTGTTCTGGACCTGTGAGGCCACCAACACTATCAATGTCCACCGGCTGGATGGGGATGCCATGGGA
  GTGGTGCTTCGAGGGGACCGTGACAAGCCAAGGGCCATTGCTGTCAATGCTGAGCGAGGGTACATGTAC
  TTTACCAACATGCAGGACCATGCTGCCAAGATCGAGCGAGCCTCCCTGGATGGCACAGAGCGGGAGGTC
  CTCTTCACCACAGGCCTCATCCGTCCCGTGGCCCTTGTGGTGGACAATGCTCTGGGCAAGCTCTTCTGG
  GTGGATGCCGACCTAAAGCGAATCGAAAGCTGTGACCTCTCTGGGGCCAACCGCCTGACCCTGGAAGAT
  GCCAACATCGTACAGCCAGTAGGTCTGACAGTGCTGGGCAGGCACCTCTACTGGATCGACCGCCAGCAG
  CAGATGATCGAGCGCGTGGAGAAGACCACTGGGGACAAGCGGACTAGGGTTCAGGGCCGTGTCACCCAC
  CTGACAGGCATCCATGCCGTGGAGGAAGTCAGCCTGGAGGAGTTCTCAGCCCATCCTTGTGCCCGAGAC
  AATGGCGGCTGCTCCCACATCTGTATCGCCAAGGGTGATGGAACACCGCGCTGCTCGTGCCCTGTCCAC
  CTGGTGCTCCTGCAGAACCTGCTGACTTGTGGTGAGCCTCCTACCTGCTCCCCTGATCAGTTTGCATGT
  ACCACTGGTGAGATCGACTGCATCCCCGGAGCCTGGCGCTGTGACGGCTTCCCTGAGTGTGCTGACCAG
  AGTGATGAAGAAGGCTGCCCAGTGTGCTCCGCCTCTCAGTTCCCCTGCGCTCGAGGCCAGTGTGTGGAC
  CTGCGGTTACGCTGCGACGGTGAGGCCGACTGCCAGGATCGCTCTGATGAAGCTAACTGCGATGCTGTC
  TGTCTGCCCAATCAGTTCCGGTGCACCAGCGGCCAGTGTGTCCTCATCAAGCAACAGTGTGACTCCTTC
  CCCGACTGTGCTGATGGGTCTGATGAGCTCATGTGTGAAATCAACAAGCCACCCTCTGATGACATCCCA
  GCCCACAGCAGTGCCATTGGGCCCGTCATTGGTATCATCCTCTCCCTCTTCGTCATGGGCGGGGTCTAC
  TTTGTCTGCCAGCGTGTGATGTGCCAGCGCTACACAGGGGCCAGTGGGCCCTTTCCCCACGAGTATGTT
  GGTGGAGCCCCTCATGTGCCTCTCAACTTCATAGCCCCAGGTGGCTCACAGCACGGTCCCTTCCCAGGC
  ATCCCGTGCAGCAAGTCCGTGATGAGCTCCATGAGCCTGGTGGGGGGGCGCGGCAGTGTGCCCCTCTAT
  GACCGGAATCACGTCACTGGGGCCTCATCCAGCAGCTCGTCCAGCACAAAGGCCACACTATATCCGCCG
  ATCCTGAACCCACCCCCGTCCCCGGCCACAGACCCCTCTCTCTACAACGTGGACGTGTTTTATTCTTCA
  GGCATCCCGGCCACCGCTAGACCATACAGGCCCTACGTCATTCGAGGTATGGCACCCCCAACAACACCG
  TGCAGCACAGATGTGTGTGACAGTGACTACAGCACCAGTCGCTGGAAGAGCAGCAAATACTACCTGGAC
  TTGAATTCGGACTCAGACCCCTACCCCCCCCCGCCCACCCCCCACAGCCAGTACCTATCTGCAGAGGAC
  AGCTGCCCACCCTCACCAGGCACTGAGAGGAGTTACTGCCACCTCTTCCCGCCCCCACCGTCCCCCTGC
  ACGGACTCGTCCTGA
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG
  GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT
  
• Restriction Sites: RsrII-NotI
• ACCN: NM_008513
• Insert Size: 4845 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC011374
• RefSeq Size: 5172 bp
• RefSeq ORF: 4845 bp
• Locus ID: 16973
• UniProt ID: Q91VN0
• Cytogenetics: 19 A
• MW: 178.9 kDa
• Gene Summary: Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins. Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration (PubMed:11956231). In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation (PubMed:15142971). During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass (PubMed:11956231). Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs (By similarity). Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development (By similarity). Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis (PubMed:11956231).[UniProtKB/Swiss-Prot Function]