Ace (NM_207624) Mouse Tagged ORF Clone

CAT#: MR211914

Ace (Myc-DDK-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), transcript variant 1

Product Images

Circular map for MR211914

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ace
• Synonyms: AW208573; CD143
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211914 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCCGCGTCCGGCCAGCGGGGGCGGTGGCCGTTGTCGCCTCCGCTCTTGATGCTGTCGCTGCTGG
  TGCTGTTGCTGCAGCCGTCGCCCGCCCCGGCACTCGACCCTGGATTGCAGCCGGGCAACTTTTCCCCGGA
  CGAGGCAGGGGCGCAGCTTTTCGCTGAAAGCTATAACTCGAGTGCCGAGGTGGTGATGTTCCAGAGCACC
  GTGGCCAGTTGGGCGCACGACACCAACATCACGGAGGAGAACGCGCGACGCCAGGAGGAAGCGGCCCTGG
  TCAGCCAGGAGTTTGCAGAGGTCTGGGGCAAGAAGGCCAAGGAGTTGTATGAGTCCATTTGGCAGAACTT
  TACTGACTCAAAGCTGCGAAGGATCATCGGATCTATTCGGACCCTAGGACCTGCCAATCTGCCCCTGGCC
  CAGCGGCAGCAGTACAACTCTCTGCTAAGCAACATGAGCAGAATCTACTCCACTGGCAAGGTCTGCTTCC
  CCAACAAGACTGCCACCTGCTGGTCCCTGGACCCAGAGCTCACCAACATCCTGGCTTCCTCACGAAGCTA
  TGCCAAGTTGTTGTTTGCCTGGGAGGGCTGGCATGATGCTGTGGGTATCCCACTGAAACCCCTCTATCAA
  GACTTTACTGCCATCAGTAACGAAGCCTACAGACAAGACGACTTCTCAGACACAGGAGCCTTCTGGCGCT
  CCTGGTATGAGTCCCCCTCCTTTGAGGAGAGTCTGGAACATATCTACCACCAACTAGAGCCCCTCTACCT
  GAACCTCCATGCCTACGTCCGCCGCGCACTGCACCGCCGCTATGGGGACAAATACGTCAATCTCAGGGGG
  CCTATTCCTGCCCATTTGCTGGGAGACATGTGGGCCCAGAGCTGGGAGAACATCTACGACATGGTAGTGC
  CTTTCCCAGACAAACCCAACCTCGATGTCACCAGTACAATGGTACAGAAGGGCTGGAACGCCACACACAT
  GTTCCGGGTATCAGAGGAATTCTTCACCTCGCTGGGGCTCTCACCCATGCCTCCTGAGTTCTGGGCGGAG
  TCAATGCTGGAGAAACCAACGGATGGACGGGAGGTGGTGTGCCACGCCTCTGCCTGGGACTTCTACAACC
  GGAAGGACTTCCGGATTAAGCAATGCACACGGGTCACGATGGAACAGCTGGCCACAGTACACCACGAGAT
  GGGCCACGTGCAGTACTACCTCCAGTACAAGGACCTGCACGTCTCTCTGCGTAGAGGTGCCAACCCTGGC
  TTCCATGAGGCCATTGGGGATGTGCTTGCACTCTCCGTCTCTACCCCTGCACATCTGCACAAAATCGGCC
  TACTGGACCATGTTACCAATGACATAGAGAGTGACATCAATTACCTGCTAAAGATGGCCCTAGAGAAAAT
  CGCCTTCTTGCCCTTTGGCTACCTGGTGGACCAGTGGCGTTGGGGGGTCTTCAGTGGACGGACCCCACCC
  TCTCGCTACAACTTCGACTGGTGGTATCTTCGAACCAAGTATCAGGGGATCTGCCCACCAGTTGCCCGGA
  ATGAAACCCATTTTGATGCTGGAGCCAAGTTTCACATCCCAAACGTGACACCGTACATCAGGTACTTCGT
  GAGCTTTGTGCTGCAGTTCCAGTTCCATCAAGCACTGTGCAAGGAGGCAGGCCACCAGGGCCCACTACAC
  CAGTGTGACATCTACCAGTCCGCCCAGGCGGGGGCCAAGCTCAAGCAGGTGTTGCAGGCTGGCTGCTCCA
  GGCCCTGGCAGGAGGTACTGAAGGACCTGGTAGGCTCAGATGCCCTGGATGCCAAGGCACTGCTGGAGTA
  CTTCCAACCGGTCAGCCAGTGGCTGGAAGAGCAGAATCAGCGGAATGGCGAAGTCCTAGGCTGGCCAGAG
  AATCAGTGGCGTCCACCGTTACCCGACAACTATCCAGAGGGCATTGACCTAGAGACTGATGAAGCCAAGG
  CTGACAGGTTCGTGGAAGAGTATGACCGGACAGCCCAAGTGTTGTTGAACGAGTACGCAGAGGCCAACTG
  GCAATATAACACCAACATTACCATAGAGGGCAGCAAGATCCTGCTTGAGAAAAGCACGGAGGTATCCAAT
  CACACCCTGAAATATGGCACCCGGGCCAAGACATTTGATGTGAGCAACTTTCAAAACTCTTCCATCAAGC
  GGATCATAAAGAAGCTTCAGAACCTGGACCGGGCAGTGCTGCCTCCCAAGGAATTAGAAGAGTACAACCA
  GATCCTGCTAGACATGGAGACAACTTACAGCTTATCCAACATTTGCTACACAAATGGCACTTGTATGCCC
  CTGGAACCTGATCTAACAAACATGATGGCCACATCCCGGAAATATGAAGAATTGCTATGGGCATGGAAGA
  GCTGGAGAGACAAGGTGGGGAGAGCCATCCTTCCTTTTTTCCCAAAGTATGTGGAGTTCTCCAACAAGAT
  TGCCAAGCTCAATGGCTACACGGATGCAGGGGATTCATGGAGATCCTTATACGAGTCTGACAACCTGGAG
  CAAGACCTGGAAAAACTGTACCAGGAGCTGCAGCCACTCTACCTGAACCTGCATGCCTATGTGCGTCGTT
  CCCTGCACCGCCACTATGGGTCCGAGTACATCAACCTGGATGGCCCCATTCCTGCCCATCTGCTAGGGAA
  CATGTGGGCGCAGACCTGGTCCAACATCTATGATTTGGTGGCGCCCTTCCCTTCCGCCCCCAATATAGAT
  GCCACGGAAGCCATGATAAAGCAGGGATGGACACCCAGAAGGATATTTAAGGAAGCTGACAATTTCTTTA
  CCTCCCTGGGGCTGTTACCTGTGCCCCCTGAGTTCTGGAACAAGTCGATGTTAGAGAAGCCCACCGATGG
  AAGGGAGGTGGTGTGCCATCCCTCAGCCTGGGACTTCTACAACGGCAAGGACTTCAGGATCAAGCAGTGT
  ACCTCTGTGAACATGGAGGACTTGGTGATAGCGCACCACGAAATGGGCCACATCCAGTATTTCATGCAGT
  ACAAAGACTTACCCGTGACTTTCCGGGAGGGTGCCAACCCTGGTTTTCATGAAGCTATTGGAGATATAAT
  GGCTCTCTCAGTGTCTACCCCCAAGCATCTATACAGTCTCAACCTGCTTAGCACTGAGGGCAGTGGCTAC
  GAGTATGACATCAACTTTCTAATGAAGATGGCCCTCGACAAGATCGCCTTTATCCCCTTCAGCTACCTCA
  TCGACCAGTGGCGCTGGAGGGTCTTTGATGGAAGCATCACCAAGGAGAACTATAACCAGGAGTGGTGGAG
  CCTCAGGCTGAAGTATCAGGGTCTGTGCCCCCCAGTGCCAAGATCCCAAGGTGACTTTGACCCAGGGTCC
  AAGTTCCACGTTCCTGCGAACGTGCCATACGTCAGGTACTTTGTCAGCTTCATCATCCAGTTCCAGTTCC
  ACGAGGCGCTGTGTCGCGCAGCCGGGCACACGGGTCCCCTGCACAAGTGTGACATCTACCAATCCAAGGA
  AGCAGGGAAGCTCCTGGCGGATGCCATGAAGCTGGGCTACAGTAAGCCGTGGCCAGAGGCCATGAAGCTG
  ATCACAGGCCAGCCTAACATGTCAGCCTCCGCCATGATGAATTACTTCAAGCCACTGACAGAATGGCTCG
  TCACCGAGAACAGGAGACATGGAGAGACACTGGGCTGGCCGGAGTACAACTGGGCGCCAAACACCGCTCG
  CGCAGAAGGCTCCACCGCAGAGTCCAACCGCGTCAATTTCCTGGGCCTGTACCTGGAGCCACAGCAGGCC
  CGCGTGGGCCAGTGGGTGCTGCTCTTCCTGGGCGTCGCCCTGCTCGTGGCCACCGTGGGTCTCGCCCATC
  GGCTCTACAACATCCGTAACCATCACAGCCTCCGCCGGCCCCACCGTGGGCCCCAGTTTGGGTCTGAGGT
  GGAGCTCAGACACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211914 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAASGQRGRWPLSPPLLMLSLLVLLLQPSPAPALDPGLQPGNFSPDEAGAQLFAESYNSSAEVVMFQST
  VASWAHDTNITEENARRQEEAALVSQEFAEVWGKKAKELYESIWQNFTDSKLRRIIGSIRTLGPANLPLA
  QRQQYNSLLSNMSRIYSTGKVCFPNKTATCWSLDPELTNILASSRSYAKLLFAWEGWHDAVGIPLKPLYQ
  DFTAISNEAYRQDDFSDTGAFWRSWYESPSFEESLEHIYHQLEPLYLNLHAYVRRALHRRYGDKYVNLRG
  PIPAHLLGDMWAQSWENIYDMVVPFPDKPNLDVTSTMVQKGWNATHMFRVSEEFFTSLGLSPMPPEFWAE
  SMLEKPTDGREVVCHASAWDFYNRKDFRIKQCTRVTMEQLATVHHEMGHVQYYLQYKDLHVSLRRGANPG
  FHEAIGDVLALSVSTPAHLHKIGLLDHVTNDIESDINYLLKMALEKIAFLPFGYLVDQWRWGVFSGRTPP
  SRYNFDWWYLRTKYQGICPPVARNETHFDAGAKFHIPNVTPYIRYFVSFVLQFQFHQALCKEAGHQGPLH
  QCDIYQSAQAGAKLKQVLQAGCSRPWQEVLKDLVGSDALDAKALLEYFQPVSQWLEEQNQRNGEVLGWPE
  NQWRPPLPDNYPEGIDLETDEAKADRFVEEYDRTAQVLLNEYAEANWQYNTNITIEGSKILLEKSTEVSN
  HTLKYGTRAKTFDVSNFQNSSIKRIIKKLQNLDRAVLPPKELEEYNQILLDMETTYSLSNICYTNGTCMP
  LEPDLTNMMATSRKYEELLWAWKSWRDKVGRAILPFFPKYVEFSNKIAKLNGYTDAGDSWRSLYESDNLE
  QDLEKLYQELQPLYLNLHAYVRRSLHRHYGSEYINLDGPIPAHLLGNMWAQTWSNIYDLVAPFPSAPNID
  ATEAMIKQGWTPRRIFKEADNFFTSLGLLPVPPEFWNKSMLEKPTDGREVVCHPSAWDFYNGKDFRIKQC
  TSVNMEDLVIAHHEMGHIQYFMQYKDLPVTFREGANPGFHEAIGDIMALSVSTPKHLYSLNLLSTEGSGY
  EYDINFLMKMALDKIAFIPFSYLIDQWRWRVFDGSITKENYNQEWWSLRLKYQGLCPPVPRSQGDFDPGS
  KFHVPANVPYVRYFVSFIIQFQFHEALCRAAGHTGPLHKCDIYQSKEAGKLLADAMKLGYSKPWPEAMKL
  ITGQPNMSASAMMNYFKPLTEWLVTENRRHGETLGWPEYNWAPNTARAEGSTAESNRVNFLGLYLEPQQA
  RVGQWVLLFLGVALLVATVGLAHRLYNIRNHHSLRRPHRGPQFGSEVELRHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207624
• ORF Size: 3939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207624.6
• RefSeq Size: 4906 bp
• RefSeq ORF: 3939 bp
• Locus ID: 11421
• UniProt ID: P09470
• Cytogenetics: 11 68.84 cM
• MW: 150.9 kDa
• Gene Summary: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.[UniProtKB/Swiss-Prot Function]