Gak (NM_153569) Mouse Tagged ORF Clone

CAT#: MR211904

Gak (Myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak)

Product Images

Circular map for MR211904

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gak
• Synonyms: D130045N16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211904 representing NM_153569
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCTGCTGCAGTCTGCGCTGGACTTCCTGGCGGGCCCTGGTTCTCTTGGCGGAGCTGCGGGCCGTG
  ATCAGAGTGACTTCGTGGGGCAGACAGTGGAGCTGGGCGAGCTGCGTCTGCGGGTGCGGCGGGTCCTGGC
  CGAGGGAGGGTTTGCTTTTGTTTATGAAGCTCAAGATCTGGGAAGTGGCAGAGAGTATGCATTAAAGCGA
  TTACTATCCAATGAAGAGGAAAAGAACAGAGCCATCATTCAGGAAGTTTGTTTCTTGAAAAAACTTTCTG
  GCCACCCCAATATTGTCCAGTTCTGCTCTGCAGCATCCATAGGAAAAGAGGAGTCGGACACTGGGCAGGC
  TGAGTTCCTCCTGCTTACGGAGCTTTGTAAAGGACAGCTGGTGGAGTTTCTCAAGAGAGTTGAATGTAAA
  GGTCCTCTGTCCTGCGACAGCATTCTGAAGATCTTCTACCAGACATGCAGAGCAGTGCAGCACATGCACA
  GGCAGAAACCACCCATCATCCACAGAGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGGGGACCAT
  TAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGTGCCCAGAAG
  CGAGCCATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACACCAGAAATTGTAGACC
  TGTATTCCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACCTGCTGTG
  TTTCCGGCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCATTCCTGTG
  AATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCAACCCAGAGGAGAGGC
  TGTCCATTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGTGAACCCCAAAGCTCC
  CATCACAGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACCACCTTGC
  GGGGGCACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTACGGTGGGTTTCTTG
  ATATTCTGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCATCCAGTC
  TGTGGCTAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATTGCAGTGATGTCATTC
  CCAGCAGAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGTTTCTGGATGCTAAGC
  ATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAAGTTTCACAACCGGGT
  CACTGAGTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACTCTATGTAGGAGCATG
  CATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATGGGAGAGCTGCATCTG
  CTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGTACATGTT
  CAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATATGTATGTGACATG
  GTGGCAGAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACCCCCGTGC
  CACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGGAGAGGAGCGTGTCAC
  CACCACGTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCATCCCCCTG
  GGCGTAACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACACTGGGAGGGAGGCTGC
  AGGCTAAGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGTGCCTCGAAACGCAAC
  CACTGTGAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTACCCAGATCTGTTCCAG
  GTGAACCTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCACCTTGGGAGAACACCA
  GCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGACATTCTGTCTAAGTT
  TGGGAAGCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTCTCCGGAG
  GCTGAGATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACTTTCTTCACACACTGG
  ATTGGCAGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGAGAGTCAGTCTGTCCT
  GATTGCAGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCCAGTGAGGAGAGGAAA
  CCAGGAGCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACTTAATATTTGATGTGG
  GCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCTGGGGCTACACTCTGA
  GGGGGACTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAACACTGACCTGTTGAGC
  TGCCTTCTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTGGTGGTGAGGCTCCAC
  TGCTGCTAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAAAGTCCCTGACACTGT
  GGACCCATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCCAAGCCTGATCTCTTT
  GGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCCACAGTGCCCCACCCC
  CATCCTGTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAAGGTAATAGCTTCATC
  CAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCTGGGCCAGCCTCCATT
  CCAGTACCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCCCCAACCCCAGCCAAA
  CCAAGTCTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCTCCAAGGCCTGCCTGC
  TGGACTTCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAACAGCCCCTGGCAGGCA
  AATCGTCCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCAGAGCCTCTGAACAGC
  TAAGGTCTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCCCAGCTTTGCCCAAAA
  GCCAAAGGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCTAAGTCTGACAAGAAG
  GGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCACTCAAATTGAAGC
  TTTTGGACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCTGCACACGGTATTGTG
  GGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAAGAAGCAG
  TACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCGTATGAACAGTATGCCAAGA
  TGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAGGCCCCTCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211904 representing NM_153569
  Red=Cloning site Green=Tags(s)
  
  MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKR
  LLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKRVECK
  GPLSCDSILKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQK
  RAMVEEEITRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPV
  NDTRYTVFHDLIRAMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPC
  GGTVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSF
  PAEGVESAIKNNIEDVRMFLDAKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSM
  HAWLREDHRNVCVVHCMDGRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDM
  VAEEPITPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPL
  GVTVQGDVLIIIYHARATLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQ
  VNLEVEVEPRDRPSREAPPWENTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPE
  AEITESDSPQSSSTDTNHFLHTLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERK
  PGAGEDTPRLAAGTKQQDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLS
  CLLEPSDAAQVGPPGDLLGGEAPLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLF
  GEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASI
  PVPEGTLFSSAGHPAPPGPNPSQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQA
  NRPTAPGTSWTPQAKPAPRASEQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKK
  GPKTMAEMRKQELARDTDPLKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQ
  YRRAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGSRPLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153569
• ORF Size: 3915 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153569.2, NP_705797.1
• RefSeq Size: 4453 bp
• RefSeq ORF: 3918 bp
• Locus ID: 231580
• UniProt ID: Q99KY4
• Cytogenetics: 5 F
• MW: 144.1 kDa
• Gene Summary: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).[UniProtKB/Swiss-Prot Function]