Flii (NM_022009) Mouse Tagged ORF Clone

CAT#: MR211879

Flii (Myc-DDK-tagged) - Mouse flightless I homolog (Drosophila) (Flii)

Product Images

Circular map for MR211879

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Flii
• Synonyms: 3632430F08Rik; Fl; Fliih
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211879 representing NM_022009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCACCGGGGTGCTGCCGTTCGTGCGCGGCGTGGACCTCAGCGGCAACGACTTCAAGGGTGGCT
  ACTTCCCTGAGAATGTTAAGGCTATGACCAGCCTGCGATGGCTAAAGCTGAATCGCACAGGCCTCTGCTA
  CCTGCCTGAAGAACTTGCGGCTCTGCAGAAGCTGGAGCACCTGTCTGTGAGCCACAACCATCTGACCACG
  CTTCACGGCGAACTGTCTAGCCTGCCTTCGCTTCGGGCCATTGTAGCTCGGGCCAACAGCCTGAAGAATT
  CCGGAGTCCCTGATGACATCTTCAAGCTGGATGATCTCTCTGTTCTGGACTTGAGCCATAACCAGTTGAC
  AGAATGCCCACGGGAGCTGGAGAATGCCAAGAACATGCTGGTGCTCAACCTCAGCCACAACGGCATCGAC
  TCCATCCCCAACCAGCTGTTCATCAACCTTACGGACTTGCTGTACCTGGACCTCAGTGAAAACCGCCTAG
  AGAGCCTTCCCCCTCAAATGCGCCGCCTCGTGCACCTTCAGACACTGGTGCTGAATGGAAACCCGCTGCT
  ACATGCACAGCTCCGGCAGCTCCCAGCCATGATGGCCCTACAGACCCTGCATCTGAGGAACACTCAACGC
  ACCCAGAGCAACCTCCCCACCAGCCTGGAGGGCCTGAGCAACCTTTCAGATGTGGACCTGTCGTGCAATG
  ACTTGACAAGGGTGCCGGAGTGCCTGTACACCCTCCCCAGCCTGCGCCGCCTCAACCTCAGTAGCAACCA
  GATTGCAGAGCTGTCCCTGTGCATCGACCAGTGGGTGCACCTGGAGACCTTGAATCTGTCCCGGAACCAG
  CTCACCTCCCTGCCTTCAGCCATCTGCAAGCTCACCAAGCTGAAGAAGCTGTACCTCAACTCCAACAAGC
  TGGACTTTGATGGGCTGCCTTCTGGCATCGGCAAGCTCACCAGCCTGGAGGAATTCATGGCTGCCAACAA
  CAACCTAGAACTGATTCCTGAAAGTCTCTGCAGGTGCCCAAAACTGAAGAAACTCGTCCTGAATAAGAAC
  CGCCTGGTGACCCTTCCTGAGGCCATCCACTTCCTGACAGAGATCCAGGTCCTGGATGTTCGAGAAAACC
  CCAGCTTGGTCATGCCGCCCAAGCCTGCTGACCGCACCGCCGAGTGGTACAACATTGACTTCTCGCTACA
  GAACCAGCTGAGGCTGGCGGGCGCCTCCCCGGCCACAGTGGCTGCTGCTGCAGCTGTAGGGAGCGGGTCC
  AAGGACCCCTTGGCTCGCAAGATGCGGCTACGGAGGCGCAAGGACTCAGCACAGGATGTCCAGGCCAAGC
  AAGTGCTAAAGGGCATGTCAGATGTTGCCCAGGAGAAAAACAAAAACCAAGAGGAAAGCATAGATGCCCG
  GGCACCAGGGGGAAAGGTACGACGCTGGGACCAGGGCCTGGAGAAGCCACGCCTTGACTACTCGGAGTTC
  TTTACGGAGGATGTGGGCCAGCTGCCTGGTTTGACCATCTGGCAAATTGAGAACTTTGTGCCTGTTCTGG
  TGGAAGAAGCCTTCCATGGCAAGTTCTACGAGGCTGACTGCTACATTGTGCTCAAGACCTTCCTGGATGA
  CAGCGGCTCCCTGAACTGGGAGATCTACTACTGGATTGGTGGGGAGGCCACACTTGACAAGAAAGCCTGT
  TCCGCCATTCATGCAGTCAATCTGCGCAACTACCTGGGTGCTGAGTGCCGTACTGTTCGAGAGGAGATGG
  GTGACGAGAGTGAGGAGTTCCTGCAGGTGTTTGACAATGACATCTCCTACATTGAAGGCGGAACAGCCAG
  TGGCTTCTATACTGTGGAAGATACACACTATGTTACCAGGATGTACCGTGTATATGGGAAAAAGAACATC
  AAATTGGAGCCTGTGCCCCTCAAAGGGTCCTCACTAGATCCAAGGTTTGTGTTTCTCCTGGACCAAGGGC
  TGGACATTTATGTGTGGCGGGGGGCCCAGGCCACACTGAGTAACACGACTAAGGCCAGGCTCTTTGCAGA
  GAAAATAAACAAGAATGAGCGGAAAGGGAAGGCGGAAATCACACTCCTGGTGCAGGGCCAAGAGCCCCCA
  GGGTTCTGGGATGTGCTGGGTGGAGAGCCTTCTGAGATCAAGAATCATGTGCCTGATGACTTTTGGCCAC
  CCCAGCCCAAGCTATACAAGGTGGGCCTGGGTCTGGGCTACTTGGAGCTTCCGCAGATCAACTACAAGCT
  CTCAGTGGAACACAAAAAACGGCCCAAGGTGGAGCTGATGCCAGGAATGAGGCTGCTGCAGAGCCTACTG
  GACACACGATGCGTGTACATCCTGGACTGCTGGTCTGATGTATTCATCTGGCTGGGCCGCAAGTCCCCAC
  GCCTAGTGCGTGCAGCAGCACTCAAACTTGGCCAGGAGCTATGTGGGATGCTACACCGGCCACGACATAC
  AGTGGTCAGCCGCAGCCTGGAGGGCACCGAGGCCCAGGTGTTCAAGGCCAAGTTCAAGAACTGGGACGAT
  GTGTTGACAGTGGACTACACACGGAATGCAGAGGCTGTGCTTCAGGGCCAGGGCCTCTCTGGGAAGGTGA
  AGCGTGACACTGAGAAGACAGACCAGATGAAGGCTGACCTCACTGCACTCTTCCTACCTCGGCAGCCCCC
  CATGCCCCTGGCTGAGGCAGAGCAGCTGATGGAGGAGTGGAATGAGGACCTGGATGGCATGGAGGGCTTC
  GTGCTGGAGGGCCGCAAGTTCACTCGACTGCCAGAGGAGGAGTTTGGCCACTTCTACACACAGGACTGCT
  ATGTCTTCCTCTGCAGGTACTGGGTACCTGTGGAGTACGAGGAGGAAGAGAAGACAGAAGACAAGGAGGG
  GAAGGCCTCGGCAGAGGCCAGAGAAGGGGAGGAAGCAGCAGCGGAGGCAGAGGAGAAGCAGCCAGAGGAG
  GACTTTCAGTGCATCGTTTACTTCTGGCAGGGCCGGGAGGCCTCCAACATGGGCTGGCTGACCTTCACAT
  TCAGTCTTCAGAAGAAGTTTGAGAGCCTCTTCCCTGGCAAGCTGGAGGTAGTACGTATGACACAGCAGCA
  GGAGAACCCCAAGTTCCTATCCCATTTCAAAAGAAAGTTCATCATTCACCGAGGCAAGAGGAAGGTGACC
  CAGGGTACTCTTCAGCCGACCCTCTATCAGATCCGCACCAACGGCAGTGCCCTCTGCACCCGGTGCATCC
  AGATTAACACTGACTCCAGCCTTCTCAACTCTGAGTTCTGCTTCATCCTCAAGGTCCCCTTTGAAAGCGA
  GGACAACCAAGGCATTGTGTATGCCTGGGTAGGTCGGGCATCAGACCCCGACGAAGCCAAGCTGGCAGAG
  GACATCCTGAACACTATGTTTGATGCATCCTACAGCAAACAGGTCATCAATGAAGGCGAGGAGCCAGAGA
  ACTTCTTCTGGGTAGGCATTGGTGCACAGAAACCCTATGATGACGATGCGGAGTACATGAAGCACACGAG
  GCTCTTCAGGTGCTCCAATGAGAAGGGTTACTTCGCAGTGACTGAGAAATGCTCTGACTTTTGCCAAGAT
  GACCTGGCAGACGATGACATCATGCTGCTAGACAATGGCCAAGAAGTCTACATGTGGGTTGGGACCCAGA
  CAAGCCAAGTGGAGATCAAACTGAGTCTGAAGGCTTGCCAGGTATACATCCAGCACACACGCTCTAAAGA
  ACATGAGCGGCCACGTCGCCTGCGCCTGGTCCGCAAAGGTAATGAGCAGCGCGCCTTCACCCGCTGCTTC
  CACGCCTGGAGCACGTTCCGCCAAGCCCCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211879 representing NM_022009
  Red=Cloning site Green=Tags(s)
  
  MEATGVLPFVRGVDLSGNDFKGGYFPENVKAMTSLRWLKLNRTGLCYLPEELAALQKLEHLSVSHNHLTT
  LHGELSSLPSLRAIVARANSLKNSGVPDDIFKLDDLSVLDLSHNQLTECPRELENAKNMLVLNLSHNGID
  SIPNQLFINLTDLLYLDLSENRLESLPPQMRRLVHLQTLVLNGNPLLHAQLRQLPAMMALQTLHLRNTQR
  TQSNLPTSLEGLSNLSDVDLSCNDLTRVPECLYTLPSLRRLNLSSNQIAELSLCIDQWVHLETLNLSRNQ
  LTSLPSAICKLTKLKKLYLNSNKLDFDGLPSGIGKLTSLEEFMAANNNLELIPESLCRCPKLKKLVLNKN
  RLVTLPEAIHFLTEIQVLDVRENPSLVMPPKPADRTAEWYNIDFSLQNQLRLAGASPATVAAAAAVGSGS
  KDPLARKMRLRRRKDSAQDVQAKQVLKGMSDVAQEKNKNQEESIDARAPGGKVRRWDQGLEKPRLDYSEF
  FTEDVGQLPGLTIWQIENFVPVLVEEAFHGKFYEADCYIVLKTFLDDSGSLNWEIYYWIGGEATLDKKAC
  SAIHAVNLRNYLGAECRTVREEMGDESEEFLQVFDNDISYIEGGTASGFYTVEDTHYVTRMYRVYGKKNI
  KLEPVPLKGSSLDPRFVFLLDQGLDIYVWRGAQATLSNTTKARLFAEKINKNERKGKAEITLLVQGQEPP
  GFWDVLGGEPSEIKNHVPDDFWPPQPKLYKVGLGLGYLELPQINYKLSVEHKKRPKVELMPGMRLLQSLL
  DTRCVYILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHTVVSRSLEGTEAQVFKAKFKNWDD
  VLTVDYTRNAEAVLQGQGLSGKVKRDTEKTDQMKADLTALFLPRQPPMPLAEAEQLMEEWNEDLDGMEGF
  VLEGRKFTRLPEEEFGHFYTQDCYVFLCRYWVPVEYEEEEKTEDKEGKASAEAREGEEAAAEAEEKQPEE
  DFQCIVYFWQGREASNMGWLTFTFSLQKKFESLFPGKLEVVRMTQQQENPKFLSHFKRKFIIHRGKRKVT
  QGTLQPTLYQIRTNGSALCTRCIQINTDSSLLNSEFCFILKVPFESEDNQGIVYAWVGRASDPDEAKLAE
  DILNTMFDASYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTRLFRCSNEKGYFAVTEKCSDFCQD
  DLADDDIMLLDNGQEVYMWVGTQTSQVEIKLSLKACQVYIQHTRSKEHERPRRLRLVRKGNEQRAFTRCF
  HAWSTFRQAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022009
• ORF Size: 3813 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022009.2
• RefSeq Size: 4012 bp
• RefSeq ORF: 3816 bp
• Locus ID: 14248
• UniProt ID: Q9JJ28
• Cytogenetics: 11 37.81 cM
• MW: 145.3 kDa
• Gene Summary: This gene encodes a protein with gelsolin-like repeats and an N-terminal leucine-rich repeat domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. This protein may act as an actin-remodelling protein as well as a transcriptional coactivator. Homozygous knockout mice show embryonic lethality. This protein may act to regulate wound repair. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]