Arhgap29 (NM_172525) Mouse Tagged ORF Clone

CAT#: MR211871

Arhgap29 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 29 (Arhgap29)

Product Images

Circular map for MR211871

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap29
• Synonyms: 6720461J18Rik; A830014I19; AU040217; B130017I01Rik; C76601; Parg1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211871 representing NM_172525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGCTCACAAACAGAAAAAGGCAAAGAAAAAGCGTGTTTGGGCATCAGGCCAACCTTCTGCTGCTA
  TTACAACTTCTGAAATGGGGCTCAAGTCCGTAAGTTCCAGCTCCAGTTTTGATCCGGAGTACATCAAGGA
  GCTGGTGAATGATGTCAGGAAGTTCTCCCATATGTTGCTATATTTGAAAGAAGCTATTCTTTCAGACTGT
  TTTAAAGAAGTCATTCATATCCGTCTGGATGAGCTTCTCCGTGTTTTAAAGTCGATATTGAGCAAGCATC
  AGAACCTCAGCTCCGTAGATCTTCAGAGCGCTGCAGAGGTGCTCACTGCAAAAGTGAAAGCTGTGAACTT
  TACAGAAGTTAATGAAGAAAACAAAAACGATATATTCCGAGAAGTCTTTTCCTCCATTGAAACATTGGCA
  TTTACCTTTGGAAACATCCTCACAAACTTCCTTATGGGAGACGTAGGCAGTGACTCGATACTACGTCTAC
  CTATTTCTCGAGAAAGTAAGTCTTTTGAAAACATTTCTGTGGACTCAGTGGACTTACCCCATGAAAAAGG
  AAATTTTTCTCCTATAGAACTAGACAACTTGCTGTTAAAGAACACTGACTCTATAGAGCTGGCTTTGTCC
  TATGCTAAAACATGGTCAAAATATACCAAGAATATAGTGTCGTGGGTTGAAAAAAAGCTCAACTTGGAAT
  TGGAGTCCACTAGAAATATTGTAAAATTGGCAGAGGCAACTAGATCTAGCATTGGTATACAAGAGTTTAT
  GCCACTGCAGTCTCTATTTACCAACGCTCTTCTCAGTGACATCCACAGCAGCCACCTTCTACAACAGACA
  ATTGCAGCCCTCCAAGCCAATAAATTTGTGCAGCCTCTACTTGGGAGGAAGAATGAGATGGAGAAGCAAA
  GGAAAGAAATAAAAGACCTTTGGAAGCAGCAACAGAATAAATTGCTCGAAACAGAGACAGCTCTCAAAAA
  AGCAAAATTGTTGTGCATGCAGCGGCAAGATGAATACGAAAAGGCAAAATCGTCCATGTTTCGTGCAGAA
  GAAGAGCAGCTAAGTTCAAGTGTTGGTTTGGCAAAAAATCTCAACAAACAACTGGAAAAAAGGCGGAGGT
  TGGAAGAAGAGGCTCTTCAAAAAGTAGAAGAAGCAAACGAACACTACAAAGTCTGTGTAACAAATGTTGA
  AGAAAGACGGAATGATCTAGAAAATACAAAGAGGGAAATTTTAACACAGCTTCGGACACTTGTTTTCCAG
  TGTGACCTTACACTTAAAGCTGTAACAGTTAACCTCTTTCATATGCAGCAGCTACAGGCTGCATCCCTTG
  CCAACAGTTTACAGTCCCTCTGTGACAGTGCCAAACTCTATGATCCAGGTCAGGAGTACAGTGAATTCGT
  GAAGGCTACAAGCTCAAGTGAATTAGAAGAAAAGGTTGATGGGAATGTAAATAAACAAATGACCAACAGT
  CCGCAGACATCTGGCTATGAACCTGCTGACTCCTTAGAGGATGTTGCCCGCCTTCCTGACAGCTGTCATA
  AGCTTGAAGAGGACAGGTGCTCCAACAGTGCAGACATGACAGGTCCTTCTTTCGTAAGATCATGGAAGTT
  CGGAATGTTTAGTGACTCAGAGAGCACTGGAGGAAGCAGTGAGTCTAGATCTCTGGATTCAGAGTCTATA
  AGTCCAGGAGACTTTCATCGGAAACTTCCACGGACTCCATCCAGTGGAACCATGTCTTCTGCTGATGATC
  TCGATGAGAGAGAGCCACCGTCCCCTTCAGAAGCTGGACCCAATTCCCTCGGAGCATTTAAGAAAACTTT
  GATGTCAAAGGCAGCTCTCACTCACAAGTTTCGCAAGTTGAGATCCCCGACAAAGTGCAGGGATTGTGAC
  GGCATCGTAATGTTCCCAGGCGTCGAGTGTGAAGAGTGTCTCCTTGTTTGTCATCGGAAGTGTCTGGAGA
  ATTTAGTCATTATTTGTGGTCATCAAAAACTTCAGGGAAAAATGCACATATTTGGAGCAGAATTCATACA
  AGTTGCAAAAAAGGAACCAGATGGCATCCCTTTTGTACTGAAAATATGTGCCTCAGAAATTGAAAATAGA
  GCCTTGTGTCTCCAGGGAATTTATCGTGTTTGTGGAAACAAAATAAAAACTGAAAAACTGTGCCAAGCTT
  TGGAAAATGGGATGCACTTAGTAGACATTTCAGAATTCAGTTCACATGACATCTGTGATGTCTTGAAATT
  ATACCTGCGACAGCTTCCAGAACCATTTATTTTATTCAGATTGTACAAGGAATTTATAGACCTTGCAAAA
  GAGATACAACATGTAAATGAAGAACAAGAGGCAAAAAAAGATAGCCCTGAAGACAAGAAACACCCACATG
  TGAGCATAGAAGTCAACCGCATCCTTCTGAAGAGCAAAGACCTGCTGAGACAGCTGCCAGCGTCACATTT
  CAACAGCCTCCATTACCTCATAGCACATCTGAGGCGAGTGGTGGATCATGCAGAAGAGAACAAGATGAAT
  TCTAAGAACTTGGGGGTGATATTTGGACCAACTCTCATTAGGCCAAGGCCTACAACGGCTCCTGTCACCA
  TCTCGTCCCTTGCTGAATATTCCAATCAGGCACGATTAGTAGAGTTCCTTATTACTTACTCACAGAAGAT
  CTTCGATGGGTCCCTCCAGCCTCAAGCTGTTGTTATATCTAACACAGGTGCTGTGGCACCTCAGGTTGAT
  CAAGGCTATCTTCCAAAACCTCTGTTATCACCAGATGAGAGAGACACAGATCATTCTATGAAACCACTCT
  TTTTTTCTTCAAAGGAAGATATCCGTAGTTCAGATTGTGAGAGCAAAAGTTTTGAATTAACTACATCTTT
  TGAAGAATCAGAACGCAGACAAAATGCATTGGGGAAATGTGACGCTCCTCTCCTCGACAACAAAGTACAT
  TTGCTTTTTGACCAAGAGCATGAGTCAGCGTCCCAAAAGATGGAAGATGTCTGTAAAAGCCCCAAGCTGC
  TGCTGCTGAAATCCAATAGGGCAGCAAACAGTGTGCAGAGACATACTCCAAGGACCAAGATGAGACCTGT
  AAGCTTGCCTGTAGACCGGCTGCTTCTTCTTGCCAGTTCTCCTACTGAGAGAAGCAGCAGGGATGTAGGA
  AACGTAGACTCAGACAAGTTTGGCAAGAACCCTGCCTTTGAAGGACTCCATAGAAAGGACAACTCAAATA
  CTACTCGCTCCAAAGTTAATGGCTTTGACCAGCAAAATGTACAGAAATCCTGGGACACACAATATGTACG
  GAACAATTTTACTGCCAAGACTACGATGATTGTTCCCAGTGCCTACCCTGAGAAGGGATTGACAGTAAAC
  ACTGGGAATAACAGGGACCATCCCGGCAGTAAAGCACATGCAGAGCCAGCCAGGGCTGCAGGAGATGTGT
  CAGAGCGCAGGTCCTCTGACTCCTGCCCCGCCACTGCTGTCAGAGCACCCAGAACACTGCAGCCCCAACA
  CTGGACAACATTTTACAAACCACCTAATCCCACCTTCAGTGTCAGGGGGACTGAGGAGAAAACAGCATTA
  CCCTCAATAGCTGTACCTCCTGTCCTGGTGCATGCTCCCCAGATCCATGTGACAAAATCAGACCCAGACT
  CAGAGGCCACATTGGCCTGTCCTGTGCAGACAAGTGGTCAACCTAAAGAGAGCTCTGAGGAGCCTGCCCT
  GCCTGAGGGGACTCCAACTTGCCAGAGACCACGACTAAAACGAATGCAGCAATTTGAAGACCTTGAAGAT
  GAAATCCCACAGTTTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211871 representing NM_172525
  Red=Cloning site Green=Tags(s)
  
  MIAHKQKKAKKKRVWASGQPSAAITTSEMGLKSVSSSSSFDPEYIKELVNDVRKFSHMLLYLKEAILSDC
  FKEVIHIRLDELLRVLKSILSKHQNLSSVDLQSAAEVLTAKVKAVNFTEVNEENKNDIFREVFSSIETLA
  FTFGNILTNFLMGDVGSDSILRLPISRESKSFENISVDSVDLPHEKGNFSPIELDNLLLKNTDSIELALS
  YAKTWSKYTKNIVSWVEKKLNLELESTRNIVKLAEATRSSIGIQEFMPLQSLFTNALLSDIHSSHLLQQT
  IAALQANKFVQPLLGRKNEMEKQRKEIKDLWKQQQNKLLETETALKKAKLLCMQRQDEYEKAKSSMFRAE
  EEQLSSSVGLAKNLNKQLEKRRRLEEEALQKVEEANEHYKVCVTNVEERRNDLENTKREILTQLRTLVFQ
  CDLTLKAVTVNLFHMQQLQAASLANSLQSLCDSAKLYDPGQEYSEFVKATSSSELEEKVDGNVNKQMTNS
  PQTSGYEPADSLEDVARLPDSCHKLEEDRCSNSADMTGPSFVRSWKFGMFSDSESTGGSSESRSLDSESI
  SPGDFHRKLPRTPSSGTMSSADDLDEREPPSPSEAGPNSLGAFKKTLMSKAALTHKFRKLRSPTKCRDCD
  GIVMFPGVECEECLLVCHRKCLENLVIICGHQKLQGKMHIFGAEFIQVAKKEPDGIPFVLKICASEIENR
  ALCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAK
  EIQHVNEEQEAKKDSPEDKKHPHVSIEVNRILLKSKDLLRQLPASHFNSLHYLIAHLRRVVDHAEENKMN
  SKNLGVIFGPTLIRPRPTTAPVTISSLAEYSNQARLVEFLITYSQKIFDGSLQPQAVVISNTGAVAPQVD
  QGYLPKPLLSPDERDTDHSMKPLFFSSKEDIRSSDCESKSFELTTSFEESERRQNALGKCDAPLLDNKVH
  LLFDQEHESASQKMEDVCKSPKLLLLKSNRAANSVQRHTPRTKMRPVSLPVDRLLLLASSPTERSSRDVG
  NVDSDKFGKNPAFEGLHRKDNSNTTRSKVNGFDQQNVQKSWDTQYVRNNFTAKTTMIVPSAYPEKGLTVN
  TGNNRDHPGSKAHAEPARAAGDVSERRSSDSCPATAVRAPRTLQPQHWTTFYKPPNPTFSVRGTEEKTAL
  PSIAVPPVLVHAPQIHVTKSDPDSEATLACPVQTSGQPKESSEEPALPEGTPTCQRPRLKRMQQFEDLED
  EIPQFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172525
• ORF Size: 3798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172525.3
• RefSeq Size: 5133 bp
• RefSeq ORF: 3801 bp
• Locus ID: 214137
• UniProt ID: Q8CGF1
• Cytogenetics: 3 G1
• MW: 142.8 kDa
• Gene Summary: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity). In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.[UniProtKB/Swiss-Prot Function]