Tpp2 (BC058239) Mouse Tagged ORF Clone

CAT#: MR211854

Tpp2 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147)

Product Images

Circular map for MR211854

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpp2
• Synonyms: TPP-2; TppII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211854 representing BC058239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCGCCGCGACCGAGGAGCCCTTTCCTTTCCACGGTCTTCTACCAAAGAAGGAGACCGGGGCCT
  CCTCCTTCCTGTGCCGCTACCCGGAGTATGACGGGCGCGGGGTGCTCATCGCCGTCCTGGACACAGGGGT
  CGATCCCGGGGCCCCGGGCATGCAGGTTACAACTGATGGAAAACCAAAAATCATTGATATCATTGATACA
  ACAGGAAGTGGTGATGTAAATACTGCTACAGAAGTAGAACCAAAAGATGGTGAAATTATTGGTCTTTCTG
  GAAGAGTGCTTAAGATTCCTGCAAACTGGACAAATCCTTTAGGAAAATATCATATTGGCATTAAAAATGG
  TTATGACTTCTATCCAAAGGCTCTCAAGGAAAGGATACAGAAAGAACGGAAGGAAAAAATCTGGGATCCA
  ATTCACAGAGTTGCACTTGCAGAAGCTTGTAGAAAACAAGAAGAATTTGATATTGCCAACAATGGCTCTT
  CCCAAGCCAATAAACTAATCAAGGAAGAGTTGCAAAGTCAAGTGGAATTACTTAATTCTTTTGAGAAAAA
  GTATAGTGATCCAGGCCCTGTATATGACTGCTTGGTGTGGCATGATGGTGAGACCTGGAGAGCCTGTGTT
  GATTCAAATGAGAATGGGGACTTGAGTAAATGTGCCGTATTGAGAAACTACAAAGAGGCCCAAGAGTACA
  GTTCTTTTGGCACAGCTGAGATGCTGAATTACTCTGTGAACATTTATGACGATGGGAACCTGCTCTCCAT
  TGTGACCAGCGGAGGAGCTCATGGAACCCATGTAGCAAGTATAGCCGCAGGGCATTTTCCAGAAGAGCCT
  GAACGGAATGGAGTTGCTCCTGGTGCTCAAATTCTATCCATTAAGATTGGTGATACACGGCTAAGCACTA
  TGGAAACAGGCACAGGCCTCATCAGAGCTATGATAGAAGTTATAAATCATAAGTGTGATCTTGTCAACTA
  CAGTTATGGAGAAGCAACTCATTGGCCAAATTCTGGGAGAATTTGTGAAGTAATTAATGAAGCAGTATGG
  AAACATAATACAATTTATGTTTCAAGTGCTGGAAATAATGGTCCATGCCTTTCTACAGTGGGTTGTCCAG
  GAGGAACTACATCCAGTGTGATAGGTGTTGGAGCTTATGTTTCCCCTGATATGATGGTTGCAGAGTATTC
  ACTGAGAGAGAAACTGCCTGCAAATCAATATACATGGTCTTCTAGAGGCCCAAGTGCTGATGGAGCCCTC
  GGTGTGAGCATCAGTGCACCAGGAGGTGCTATTGCTTCTGTGCCTAACTGGACATTGAGGGGGACTCAGC
  TAATGAATGGGACATCAATGTCTTCCCCCAATGCCTGTGGTGGCATTGCCCTGGTACTTTCAGGGCTGAA
  AGCAAATAATGTTGACTATACTGTACACTCAGTCAGAAGAGCTCTAGAAAATACTGCAATAAAAGCTGAC
  AATATAGAAGTATTTGCCCAAGGACATGGAATTATTCAGGTTGACAAAGCTTATGACTACCTCATTCAAA
  ATACATCATTTGCTAACAGATTAGGTTTTACAGTTACTGTTGGAAATAACCGTGGTATCTACCTCCGAGA
  TCCTGTCCAGGTGGCTGCTCCTTCAGATCATGGTGTTGGCATTGAGCCTGTATTTCCAGAGAACACAGAA
  AACTCTGAAAAAATATCCTTTCAGCTTCATTTAGCTTTAACTTCAAATTCATCTTGGGTTCAGTGTCCCA
  GCCATTTGGAACTCATGAATCAGTGTCGGCACATAAACATACGTGTGGACCCCAGGGGCTTAAGAGAAGG
  GTTACATTATACAGAGGTATGTGGCTATGATATAGCATCCCCCAATGCAGGTCCTTTATTCAGAGTTCCA
  ATCACTGCAGTTATAGCAGCAAAAGTAAATGAGTCATCACATTATGATCTAGCCTTTACAGATGTACATT
  TTAAACCTGGTCAGATTCGAAGACATTTTGTTGAGGTTCCTGAAGGGGCAACCTGGGCTGAAGTTACCGT
  GTGTTCATGTTCTTCTGAGGTATCGGCAAAATTTGTTCTTCATGCAGTACAGCTTGTGAAGCAGAGAGCA
  TATCGAAGTCATGAATTTTATAAGTTTTGTTCCCTTCCAGAAAAAGGAACACTTATTGAAGCTTTTCCTG
  TTTTGGGCGGAAAAGCAATTGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTTAGTGATGTCAATATTGA
  TTATACCATATCATTCCATGGGATAGTGTGTACTGCACCACAGTTAAACATTCATGCATCTGAAGGAATC
  AATCGTTTTGATGTTCAGTCCTCTTTAAAGTATGAAGATCTGGCTCCTTGCATAACTTTGAAGAGCTGGG
  TGCAAACGCTACGCCCAGTAAATGCAAAAACCAGACCTTTAGGATCAAGAGATGTTTTGCCAAATAATCG
  CCAGCTTTATGAGATGGTCCTTACATACAGCTTTCATCAGCCCAAGAGCGGAGAAGTAACACCTAGTTGT
  CCACTCCTTTGTGAATTGTTATATGAGTCAGAATTTGACAGTCAGTTGTGGATTATTTTCGACCAGAACA
  AAAGACAGATGGGCTCAGGCGATGCCTATCCACATCAGTATTCTCTGAAATTGGAGAAAGGAGATTATAC
  AATTCGATTACAGATTCGTCATGAGCAAATCAGTGATTTGGATCGTCTCAAAGATCTTCCGTTTATTGTT
  TCACATAGGTTGTCTAATACCTTGAGCTTAGATATTCATGAAAATCATAGCCTTGCACTTCTAGGAAAGA
  AGAAATCAAGCAGCTTAACATTACCACCCAAATACAATCAGCCATTCTTTGTTACTTCCTTACCTGATGA
  TAAAATACCTAAGGGGGCAGGACCCGGATGCTACCTTGCAGGCTCCTTGACATTGTCAAAGACTGAGCTT
  GGAAAGAAAGCTGATGTGATCCCTGTTCATTACTATCTCATACCTCCACCAACAAAGATTAAGAATGGCA
  GCAAAGATAAAGAAAAGGATTCAGAAAAAGAGAAAGACTTGAAAGAAGAGTTTACTGAAGCATTACGCGA
  TCTCAAAATTCAGTGGATGACCAAGCTTGATTCTACTGACATTTACAATGAATTGAAAGAAACATATCCT
  GCTTACCTTCCTTTGTATGTTGCACGTCTTCATCAACTGGATGCTGAAAAGGAACGAATGAAAAGACTTA
  ATGAAATTGTTGATGCTGCCAATGCTGTTATTTCTCACATCGATCAAACCGCTCTTGCAGTTTACATTGC
  AATGAAGACTGACCCCAGGCCTGATGCAGCTACTATAAAAAATGATATGGACAAGCAGAAATCCACCCTG
  ATAGATGCCCTCTGCAGGAAAGGATGTGCCCTGGCAGATCACCTTCTTCATACACAGCCTCACGACGGGG
  CAGCAGCTGGAGATGCTGAAGCAAAGGAAGAAGAAGGAGAAAGTACCATGGAATCTCTATCGGAAACCTA
  TTGGGAAACTACAAAGTGGACAGATCTTTTTGACACTAAGGTTTTGATATTTGCATACAAGCATGCATTA
  GTAAATAAGATGTATGGGAGAGGCCTTAAGTTTGCAACCAAACTCGTAGAAGAAAAACCAACAAAAGAAA
  ACTGGAAAAATTGTATTCAACTCATGAAATTACTTGGATGGACCCACTGTGCATCTTTTACTGAAAACTG
  GCTCCCCATCATGTATCCTCCTGATTATTGTGTATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211854 representing BC058239
  Red=Cloning site Green=Tags(s)
  
  MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDT
  TGSGDVNTATEVEPKDGEIIGLSGRVLKIPANWTNPLGKYHIGIKNGYDFYPKALKERIQKERKEKIWDP
  IHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACV
  DSNENGDLSKCAVLRNYKEAQEYSSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEP
  ERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVW
  KHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGAL
  GVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKAD
  NIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTE
  NSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVP
  ITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRA
  YRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGI
  NRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSC
  PLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIV
  SHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTEL
  GKKADVIPVHYYLIPPPTKIKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYP
  AYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTL
  IDALCRKGCALADHLLHTQPHDGAAAGDAEAKEEEGESTMESLSETYWETTKWTDLFDTKVLIFAYKHAL
  VNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058239
• ORF Size: 3747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058239.1
• RefSeq Size: 3960 bp
• RefSeq ORF: 3749 bp
• Locus ID: 22019
• Cytogenetics: 1 23.5 cM
• MW: 145.2 kDa
• Gene Summary: Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). Stimulates adipogenesis.[UniProtKB/Swiss-Prot Function]