Zeb2 (NM_015753) Mouse Tagged ORF Clone

CAT#: MR211811

Zeb2 (Myc-DDK-tagged) - Mouse zinc finger E-box binding homeobox 2 (Zeb2), transcript variant 2

Product Images

Circular map for MR211811

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zeb2
• Synonyms: 9130203F04Rik; D130016B08Rik; SIP1; Zfhx1b; Zfx1b; Zfxh1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211811 representing NM_015753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGCCGATCATGGCGGATGGCCCCCGGTGCAAGAGGCGCAAACAAGCCAATCCCAGGAGGAAAA
  ACGTGGTGAACTATGACAACGTAGTGGACGCAGGCTCGGAGACAGATGAAGAGGACAAGCTTCACATTGC
  TGAAGATGATAGCCTTGCAAACCCTCTGGACCAGGACACCAGCCCAGCTAGCATGCCCAACCATGAGTCC
  TCCCCACACATGAGCCAAGGGCTGCTACCAAGAGAGGAAGAAGAGGAGGAGCTAAGGGAGAGTGTTGTGG
  AGCACAGCTGGCACAGTGGCGAGATTCTGCAAGCCTCTGTAGCCGGTCCAGAAGAAATGAAGGAAGATTA
  TGATGCAATGGGGCCAGAAGCCACGATCCAGACCACAATTAACAATGGTACAGTTAAGAATGCAAATTGC
  ACATCAGACTTTGAGGAATACTTTGCCAAAAGAAAACTGGAGGAAAGAGATGGCCACGCAGTGAGCATCG
  AAGAGTACCTTCAGCGAAGCGACACGGCCATTATTTACCCAGAAGCCCCTGAGGAGCTGTCTCGCCTTGG
  CACGCCAGAGGCCAACGGGCAAGAAGAAAATGACCTGCCACCTGGAACTCCAGATGCTTTTGCCCAACTG
  CTGACCTGCCCCTACTGCGACCGCGGCTACAAGCGCTTGACATCGCTGAAGGAGCACATCAAGTACCGCC
  ACGAGAAGAATGAAGAGAACTTTTCCTGCCCTCTTTGTAGCTACACGTTCGCCTACCGCACCCAGCTCGA
  GAGGCATATGGTGACGCACAAGCCAGGGACAGATCAGCACCAAATGCTAACCCAAGGAGCAGGTAACCGC
  AAGTTCAAGTGCACGGAGTGTGGCAAGGCCTTCAAGTACAAGCACCACCTGAAAGAACACCTGAGAATTC
  ACAGTGGTGAAAAACCTTACGAATGCCCAAACTGCAAGAAACGCTTCTCTCATTCTGGGTCCTACAGTTC
  ACATATCAGCAGCAAGAAATGTATTGGTTTAATATCAGTAAATGGCCGAATGAGAAACAATATCAAGACG
  GGTTCTTCCCCTAATTCTGTTTCTTCTTCTCCTACTAACTCAGCCATTACTCAGTTAAGGAACAAGTTGG
  AAAATGGAAAACCACTTAGCATGTCTGAGCAGACAGGCTTACTTAAGATTAAAACAGAACCACTAGACTT
  CAATGACTATAAAGTTCTTATGGCAACACATGGGTTTAGTGGCAGCAGTCCCTTTATGAACGGTGGGCTT
  GGAGCCACCAGCCCTTTAGGTGTACACCCATCTGCTCAGAGTCCAATGCAGCACTTAGGTGTAGGGATGG
  AAGCCCCTTTACTTGGATTTCCCACTATGAATAGTAACTTGAGTGAGGTACAAAAGGTTCTACAGATTGT
  GGACAATACGGTTTCTAGGCAAAAGATGGACTGCAAGACGGAAGACATTTCAAAGTTGAAAGGTTATCAC
  ATGAAGGATCCATGTTCTCAGCCAGAAGAACAAGGGGTAACTTCTCCCAATATTCCCCCTGTCGGTCTTC
  CAGTAGTGAGTCATAACGGTGCCACTAAAAGTATTATTGACTATACCTTAGAGAAAGTCAATGAAGCCAA
  AGCTTGCCTCCAGAGCTTGACCACCGACTCAAGGAGACAGATCAGTAACATAAAGAAAGAGAAGTTGCGT
  ACTTTGATAGATTTGGTCACTGATGATAAAATGATTGAGAACCACAGCATATCCACTCCATTTTCATGCC
  AGTTCTGTAAAGAAAGCTTCCCGGGCCCTATTCCCCTGCATCAGCATGAACGATACCTGTGTAAGATGAA
  TGAAGAGATCAAGGCAGTCCTGCAACCTCATGAAAACATAGTCCCCAACAAAGCTGGAGTTTTTGTTGAT
  AATAAAGCCCTCCTCTTGTCATCTGTACTTTCCGAGAAAGGACTGACAAGCCCCATCAACCCATACAAGG
  ACCACATGTCTGTACTGAAAGCATACTATGCTATGAACATGGAGCCCAACTCTGATGAACTGCTGAAAAT
  CTCCATTGCTGTGGGCCTTCCTCAGGAATTTGTGAAGGAATGGTTTGAGCAAAGAAAAGTCTACCAGTAT
  TCGAATTCCAGGTCACCATCACTGGAAAGGACCTCCAAGCCGTTAGCTCCCAACAGTAACCCCACCACAA
  AAGACTCTTTGTTACCCAGGTCTCCTGTAAAACCTATGGACTCCATCACATCGCCATCTATAGCAGAACT
  CCACAACAGTGTTACGAGTTGTGATCCTCCTCTCAGGCTAACAAAATCTTCCCATTTCACCAATATTAAA
  GCAGTTGATAAACTGGACCACTCGAGGAGTAATACTCCTTCTCCTTTAAATCTTTCCTCCACATCTTCTA
  AAAACTCCCACAGTAGCTCGTACACTCCAAATAGCTTCTCTTCCGAGGAGCTGCAGGCTGAGCCGTTGGA
  CCTGTCATTACCAAAACAAATGAGAGAACCCAAAGGTATTATAGCCACAAAGAACAAAACAAAAGCTACT
  AGCATAAACTTAGACCACAACAGTGTTTCTTCATCGTCTGAGAATTCAGATGAGCCTCTGAATTTGACTT
  TTATCAAGAAAGAGTTTTCAAATTCTAATAACCTGGACAATAAAAGCAACAACCCTGTGTTCGGCATGAA
  CCCATTTAGTGCCAAGCCTTTATACACCCCTCTTCCACCACAGAGCGCATTTCCCCCTGCCACTTTCATG
  CCACCAGTCCAGACCAGCATCCCCGGGCTACGACCATACCCAGGACTGGATCAGATGAGCTTCCTACCGC
  ATATGGCCTATACCTACCCAACGGGAGCAGCTACCTTTGCTGATATGCAGCAAAGGAGGAAATACCAGAG
  GAAACAAGGATTTCAGGGAGACTTGCTGGATGGAGCACAAGACTACATGTCAGGCCTAGATGACATGACA
  GACTCCGATTCCTGTCTGTCTCGAAAGAAGATAAAGAAGACAGAAAGTGGCATGTATGCATGTGACTTAT
  GTGACAAGACATTCCAGAAAAGCAGTTCCCTTCTGCGACATAAATACGAACACACAGGAAAGAGACCACA
  CCAGTGTCAGATTTGTAAGAAAGCGTTCAAACACAAACACCACCTTATCGAGCACTCGAGGCTGCACTCG
  GGCGAGAAGCCCTATCAGTGTGACAAATGTGGCAAGCGCTTCTCACACTCGGGCTCCTACTCGCAGCACA
  TGAATCACAGGTACTCCTACTGCAAGCGGGAGGCGGAGGAGCGGGAAGCAGCCGAGCGCGAGGCGCGAGA
  GAAAGGGCACTTGGAACCCACCGAGCTGCTGATGAACCGGGCTTACCTGCAGAGCATCACCCCTCAGGGG
  TACTCTGACTCGGAGGAGAGGGAGAGCATGCCGAGGGATGGCGAGAGCGAGAAGGAGCACGAGAAGGAGG
  GCGAGGAGGGTTATGGGAAGCTGCGGAGAAGGGACGGCGACGAGGAGGAAGAGGAGGAAGAGGAAGAAAG
  TGAAAATAAAAGTATGGATACGGATCCCGAAACGATACGGGATGAGGAAGAGACTGGGGATCACTCGATG
  GACGACAGTTCAGAGGATGGGAAAATGGAAACCAAATCAGACCACGAGGAAGACAATATGGAAGATGGCA
  TGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211811 representing NM_015753
  Red=Cloning site Green=Tags(s)
  
  MKQPIMADGPRCKRRKQANPRRKNVVNYDNVVDAGSETDEEDKLHIAEDDSLANPLDQDTSPASMPNHES
  SPHMSQGLLPREEEEEELRESVVEHSWHSGEILQASVAGPEEMKEDYDAMGPEATIQTTINNGTVKNANC
  TSDFEEYFAKRKLEERDGHAVSIEEYLQRSDTAIIYPEAPEELSRLGTPEANGQEENDLPPGTPDAFAQL
  LTCPYCDRGYKRLTSLKEHIKYRHEKNEENFSCPLCSYTFAYRTQLERHMVTHKPGTDQHQMLTQGAGNR
  KFKCTECGKAFKYKHHLKEHLRIHSGEKPYECPNCKKRFSHSGSYSSHISSKKCIGLISVNGRMRNNIKT
  GSSPNSVSSSPTNSAITQLRNKLENGKPLSMSEQTGLLKIKTEPLDFNDYKVLMATHGFSGSSPFMNGGL
  GATSPLGVHPSAQSPMQHLGVGMEAPLLGFPTMNSNLSEVQKVLQIVDNTVSRQKMDCKTEDISKLKGYH
  MKDPCSQPEEQGVTSPNIPPVGLPVVSHNGATKSIIDYTLEKVNEAKACLQSLTTDSRRQISNIKKEKLR
  TLIDLVTDDKMIENHSISTPFSCQFCKESFPGPIPLHQHERYLCKMNEEIKAVLQPHENIVPNKAGVFVD
  NKALLLSSVLSEKGLTSPINPYKDHMSVLKAYYAMNMEPNSDELLKISIAVGLPQEFVKEWFEQRKVYQY
  SNSRSPSLERTSKPLAPNSNPTTKDSLLPRSPVKPMDSITSPSIAELHNSVTSCDPPLRLTKSSHFTNIK
  AVDKLDHSRSNTPSPLNLSSTSSKNSHSSSYTPNSFSSEELQAEPLDLSLPKQMREPKGIIATKNKTKAT
  SINLDHNSVSSSSENSDEPLNLTFIKKEFSNSNNLDNKSNNPVFGMNPFSAKPLYTPLPPQSAFPPATFM
  PPVQTSIPGLRPYPGLDQMSFLPHMAYTYPTGAATFADMQQRRKYQRKQGFQGDLLDGAQDYMSGLDDMT
  DSDSCLSRKKIKKTESGMYACDLCDKTFQKSSSLLRHKYEHTGKRPHQCQICKKAFKHKHHLIEHSRLHS
  GEKPYQCDKCGKRFSHSGSYSQHMNHRYSYCKREAEEREAAEREAREKGHLEPTELLMNRAYLQSITPQG
  YSDSEERESMPRDGESEKEHEKEGEEGYGKLRRRDGDEEEEEEEEESENKSMDTDPETIRDEEETGDHSM
  DDSSEDGKMETKSDHEEDNMEDGME
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015753
• ORF Size: 3645 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015753.4
• RefSeq Size: 9113 bp
• RefSeq ORF: 3648 bp
• Locus ID: 24136
• UniProt ID: Q9R0G7
• Cytogenetics: 2 B
• MW: 137.1 kDa
• Gene Summary: Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.[UniProtKB/Swiss-Prot Function]