Nos3 (BC052636) Mouse Tagged ORF Clone

CAT#: MR211798

Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765)

Product Images

Circular map for MR211798

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nos3
• Synonyms: eNOS, ecNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211798 representing BC052636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGTTTAG
  GGCTGTGCGGCAAGCAGGGCCCAGCCTCTCCAGCACCGGAGCCTAGCCAGGCGCCAGCACCCCCGTCCCC
  AACCCGACCAGCACCAGACCACAGCCCCCCGCTAACCCGGCCCCCAGACGGACCCAGGTTTCCTCGAGTA
  AAGAATTGGGAAGTGGGCAGCATCACCTACGACACCCTCAGTGCCCAGGCTCAGCAGGATGGGCCCTGTA
  CCTCAAGACGCTGCTTGGGATCCCTGGTGTTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
  ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCCATCAAAAGGAGT
  GGCTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAGGCTGAGGTGGCAGCCACAGGCACCTACCAGC
  TCCGGGAGAGCGAGCTGGTGTTTGGGGCCAAGCAGGCCTGGCGCAATGCTCCCCGCTGTGTGGGCCGGAT
  CCAGTGGGGAAAGCTGCAGGTATTTGATGCTCGGGACTGCAGGACTGCACAGGAAATGTTCACCTACATC
  TGTAACCACATTAAATACGCAACAAATAGAGGCAATCTTCGTTCAGCCATCACAGTGTTCCCCCAGCGCT
  GCCCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGATACGCTATGCGGGCTATAGGCAGCAGGA
  TGGCTCCGTGCGAGGGGACCCCGCCAACGTGGAGATCACTGAGCTCTGTATCCAACATGGCTGGACCCCA
  GGAAATGGCCGCTTTGATGTGCTGCCCCTGTTACTCCAGGCTCCTGATGAGCCCCCAGAACTCTTCACTC
  TGCCCCCAGAGATGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTCGAGTGGTTTGCTGCCCTTGGCCT
  GCGCTGGTATGCCCTCCCAGCTGTGTCCAACATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCTGCTGCC
  CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGCTACAACA
  TACTTGAGGATGTGGCTGTGTGCATGGATCTGGACACCAGGACAACCTCATCCCTGTGGAAAGACAAGGC
  AGCGGTGGAAATTAATGTGGCCGTGTTGCACAGTTACCAGCTGGCCAAAGTGACCATAGTGGACCACCAC
  GCCGCCACAGCCTCCTTCATGAAGCACCTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCTGCCGATT
  GGGCCTGGATTGTGCCCCCCATCTCAGGCAGCCTAACTCCTGTCTTCCATCAAGAGATGGTCAACTATTT
  CCTGTCCCCTGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGAAGTGCAGCAAAGGGGGCAGGCATCACC
  AGGAAGAAGACCTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
  CGAAGCGTGTGAAGGCAACCATTCTGTATGGCTCTGAGACTGGCCGGGCCCAGAGCTACGCACAGCAGCT
  GGGAAGACTCTTCCGGAAGGCGTTTGATCCCCGGGTCCTGTGCATGGATGAGTATGATGTGGTGTCCCTA
  GAGCACGAGGCACTGGTGTTGGTGGTGACCAGCACATTTGGCAATGGGGATCCTCCGGAGAATGGAGAGA
  GCTTTGCAGCAGCGCTCATGGAAATGTCAGGCCCGTACAACAGCTCCCCTAGGCCTGAGCAGCACAAGAG
  CTACAAAATCCGATTCAACAGTGTCTCCTGCTCAGACCCACTGGTATCCTCTTGGCGGCGCAAGAGGAAG
  GAGTCTAGCAACACAGACAGTGCAGGAGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCC
  GAGCATACCCCCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
  ACTACTGCAGCTGGGCCAAGGTGATGAGCTCTGTGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCC
  GCCTTCCAGGCTGCCTGTGAAACCTTCTGTGTGGGAGAAGATGCCAAAGCTGCTGCCCGAGATATCTTCA
  GCCCCAAACGCAGCTGGAAGCGCCAGAGGTACCGGCTGAGTACCCAGGCTGAGAGCCTGCAATTACTACC
  AGGGCTGACTCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCTCTCTGTGGAAAACCTACAGAGC
  AGCAAATCCACCCGAGCCACGATCCTGGTGCGTCTGGACACCGGAGGCCAGGAGGGACTGCAGTACCAGC
  CAGGGGACCACATAGGTGTGTGCCCACCCAACCGTCCTGGCCTAGTGGAGGCACTGCTGAGCCGAGTGGA
  GGACCCTCCGCCATCCACAGAACCTGTGGCTGTGGAACAACTGGAGAAAGGCAGCCCTGGTGGCCCTCCC
  CCCGGCTGGGTACGGGACCCCCGGCTACCCCCATGTACGCTGCGGCAGGCTCTCACCTACTTCCTGGACA
  TCACTTCCCCGCCTAGTCCTCGCCTCCTTCGACTGCTCAGCACCCTGGCAGAAGAGTCCAGCGAACAGCA
  GGAGCTAGAGGCTCTCAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCAGCTGCCCCACACTG
  CTAGAGGTGCTGGAGCAATTTCCTTCAGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCTTGC
  TCCAGCCCCGGTACTACTCTGTCAGTTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACCATAGC
  TGTGCTGGCTTACAGAACCCAGGATGGGCTGGGCCCTCTGCACTACGGTGTCTGCTCCACGTGGATGAGC
  CAGCTCAAGGCGGGAGATCCAGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
  CTAACTTGCCCTGCATCCTGGTGGGCCCAGGGACTGGCATTGCACCCTTCCGGGGATTCTGGCAAGACAG
  ACTACACGACATTGAGATCAAAGGGCTACAACCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
  CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGGGTGTTTGGACAAGTCCTCA
  CCGCCTTTTCCAGGGATCCTGGCAGCCCCAAGACCTACGTGCAAGACCTCCTGAGGACAGAGCTAGCCGC
  GGAGGTTCACCGTGTGCTGTGCCTTGAGCAAGGACATATGTTTGTCTGCGGCGATGTCACTATGGCAACC
  AGCGTCCTGCAAACCGTGCAGAGAATTCTGGCAACAGAGGGCGGCATGGAGCTGGATGAAGCCGGTGACG
  TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
  GGTGACAAGCCGCATACGCACCCAGAGCTTTTCTTTGCAGGAGCGACAGCTGAGGGGCGCAGTGCCCTGG
  TCCTTTGACCCGCCTGGCCCAGAAATACCTGGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211798 representing BC052636
  Red=Cloning site Green=Tags(s)
  
  MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPAPPSPTRPAPDHSPPLTRPPDGPRFPRV
  KNWEVGSITYDTLSAQAQQDGPCTSRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRS
  GSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYI
  CNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLIRYAGYRQQDGSVRGDPANVEITELCIQHGWTP
  GNGRFDVLPLLLQAPDEPPELFTLPPEMVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAA
  PFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHH
  AATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGAGIT
  RKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSL
  EHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRK
  ESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQA
  AFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQS
  SKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPP
  PGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFSCPTL
  LEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTIAVLAYRTQDGLGPLHYGVCSTWMS
  QLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCS
  QLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMAT
  SVLQTVQRILATEGGMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPW
  SFDPPGPEIPGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC052636
• ORF Size: 3606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052636.1
• RefSeq Size: 3982 bp
• RefSeq ORF: 3608 bp
• Locus ID: 18127
• Cytogenetics: 5 11.32 cM
• MW: 146 kDa
• Gene Summary: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.[UniProtKB/Swiss-Prot Function]