Nos3 (BC052636) Mouse Untagged Clone

CAT#: MC223060

Nos3 (untagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nos3
• Synonyms: eNOS, ecNOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC052636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGTTTAG
  GGCTGTGCGGCAAGCAGGGCCCAGCCTCTCCAGCACCGGAGCCTAGCCAGGCGCCAGCACCCCCGTCCCC
  AACCCGACCAGCACCAGACCACAGCCCCCCGCTAACCCGGCCCCCAGACGGACCCAGGTTTCCTCGAGTA
  AAGAATTGGGAAGTGGGCAGCATCACCTACGACACCCTCAGTGCCCAGGCTCAGCAGGATGGGCCCTGTA
  CCTCAAGACGCTGCTTGGGATCCCTGGTGTTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
  ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCCATCAAAAGGAGT
  GGCTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAGGCTGAGGTGGCAGCCACAGGCACCTACCAGC
  TCCGGGAGAGCGAGCTGGTGTTTGGGGCCAAGCAGGCCTGGCGCAATGCTCCCCGCTGTGTGGGCCGGAT
  CCAGTGGGGAAAGCTGCAGGTATTTGATGCTCGGGACTGCAGGACTGCACAGGAAATGTTCACCTACATC
  TGTAACCACATTAAATACGCAACAAATAGAGGCAATCTTCGTTCAGCCATCACAGTGTTCCCCCAGCGCT
  GCCCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGATACGCTATGCGGGCTATAGGCAGCAGGA
  TGGCTCCGTGCGAGGGGACCCCGCCAACGTGGAGATCACTGAGCTCTGTATCCAACATGGCTGGACCCCA
  GGAAATGGCCGCTTTGATGTGCTGCCCCTGTTACTCCAGGCTCCTGATGAGCCCCCAGAACTCTTCACTC
  TGCCCCCAGAGATGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTCGAGTGGTTTGCTGCCCTTGGCCT
  GCGCTGGTATGCCCTCCCAGCTGTGTCCAACATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCTGCTGCC
  CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGCTACAACA
  TACTTGAGGATGTGGCTGTGTGCATGGATCTGGACACCAGGACAACCTCATCCCTGTGGAAAGACAAGGC
  AGCGGTGGAAATTAATGTGGCCGTGTTGCACAGTTACCAGCTGGCCAAAGTGACCATAGTGGACCACCAC
  GCCGCCACAGCCTCCTTCATGAAGCACCTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCTGCCGATT
  GGGCCTGGATTGTGCCCCCCATCTCAGGCAGCCTAACTCCTGTCTTCCATCAAGAGATGGTCAACTATTT
  CCTGTCCCCTGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGAAGTGCAGCAAAGGGGGCAGGCATCACC
  AGGAAGAAGACCTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
  CGAAGCGTGTGAAGGCAACCATTCTGTATGGCTCTGAGACTGGCCGGGCCCAGAGCTACGCACAGCAGCT
  GGGAAGACTCTTCCGGAAGGCGTTTGATCCCCGGGTCCTGTGCATGGATGAGTATGATGTGGTGTCCCTA
  GAGCACGAGGCACTGGTGTTGGTGGTGACCAGCACATTTGGCAATGGGGATCCTCCGGAGAATGGAGAGA
  GCTTTGCAGCAGCGCTCATGGAAATGTCAGGCCCGTACAACAGCTCCCCTAGGCCTGAGCAGCACAAGAG
  CTACAAAATCCGATTCAACAGTGTCTCCTGCTCAGACCCACTGGTATCCTCTTGGCGGCGCAAGAGGAAG
  GAGTCTAGCAACACAGACAGTGCAGGAGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCC
  GAGCATACCCCCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
  ACTACTGCAGCTGGGCCAAGGTGATGAGCTCTGTGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCC
  GCCTTCCAGGCTGCCTGTGAAACCTTCTGTGTGGGAGAAGATGCCAAAGCTGCTGCCCGAGATATCTTCA
  GCCCCAAACGCAGCTGGAAGCGCCAGAGGTACCGGCTGAGTACCCAGGCTGAGAGCCTGCAATTACTACC
  AGGGCTGACTCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCTCTCTGTGGAAAACCTACAGAGC
  AGCAAATCCACCCGAGCCACGATCCTGGTGCGTCTGGACACCGGAGGCCAGGAGGGACTGCAGTACCAGC
  CAGGGGACCACATAGGTGTGTGCCCACCCAACCGTCCTGGCCTAGTGGAGGCACTGCTGAGCCGAGTGGA
  GGACCCTCCGCCATCCACAGAACCTGTGGCTGTGGAACAACTGGAGAAAGGCAGCCCTGGTGGCCCTCCC
  CCCGGCTGGGTACGGGACCCCCGGCTACCCCCATGTACGCTGCGGCAGGCTCTCACCTACTTCCTGGACA
  TCACTTCCCCGCCTAGTCCTCGCCTCCTTCGACTGCTCAGCACCCTGGCAGAAGAGTCCAGCGAACAGCA
  GGAGCTAGAGGCTCTCAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCAGCTGCCCCACACTG
  CTAGAGGTGCTGGAGCAATTTCCTTCAGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCTTGC
  TCCAGCCCCGGTACTACTCTGTCAGTTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACCATAGC
  TGTGCTGGCTTACAGAACCCAGGATGGGCTGGGCCCTCTGCACTACGGTGTCTGCTCCACGTGGATGAGC
  CAGCTCAAGGCGGGAGATCCAGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
  CTAACTTGCCCTGCATCCTGGTGGGCCCAGGGACTGGCATTGCACCCTTCCGGGGATTCTGGCAAGACAG
  ACTACACGACATTGAGATCAAAGGGCTACAACCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
  CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGGGTGTTTGGACAAGTCCTCA
  CCGCCTTTTCCAGGGATCCTGGCAGCCCCAAGACCTACGTGCAAGACCTCCTGAGGACAGAGCTAGCCGC
  GGAGGTTCACCGTGTGCTGTGCCTTGAGCAAGGACATATGTTTGTCTGCGGCGATGTCACTATGGCAACC
  AGCGTCCTGCAAACCGTGCAGAGAATTCTGGCAACAGAGGGCGGCATGGAGCTGGATGAAGCCGGTGACG
  TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
  GGTGACAAGCCGCATACGCACCCAGAGCTTTTCTTTGCAGGAGCGACAGCTGAGGGGCGCAGTGCCCTGG
  TCCTTTGACCCGCCTGGCCCAGAAATACCTGGTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: BC052636
• Insert Size: 3609 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC052636, AAH52636
• RefSeq Size: 3982 bp
• RefSeq ORF: 3608 bp
• Locus ID: 18127
• Cytogenetics: 5 11.32 cM
• Gene Summary: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.[UniProtKB/Swiss-Prot Function]