Tyk2 (NM_018793) Mouse Tagged ORF Clone

CAT#: MR211764

Tyk2 (Myc-DDK-tagged) - Mouse tyrosine kinase 2 (Tyk2), transcript variant 2

Product Images

Circular map for MR211764

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tyk2
• Synonyms: JTK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211764 representing NM_018793
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGGGACCATGCCTCTGTGTGGGCGGAGAGCCATCTTGGAAGACAGCAAGGCAGATGGCACAGAAG
  CTCAGCCCCTGGTGCCCACAGGATGCTTGATGGTGCTACTGCATTGGCCTGGGCCTGAGGGCGGGGAGCC
  CTGGGTCACCTTCAGCCAGACATCTCTGACTGCAGAGGAGGTCTGCATCCACATCGCACACAAAGTCGGC
  ATCACTCCACCCTGCTTGAATCTCTTCGCACTCTACAATGCACAGGCTAAGGTCTGGCTGCCCCCAAACC
  ATATTCTGGATACATCCCAAGACATGAACCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTGGCATGG
  CATGAATCCCCAGGAGCCAGCTGTATACCGGTGTGGTTTCCCAGGGGCAGAGACTTCCTCAGACCGGGCA
  GAGCAAGGTGTACAGCTCTTGGACTCTGCCTCGTTTGAATACCTTTTTGAGCAGGGAAAGCATGAGTTCA
  TGAACGATGTGGTGTCTCTGCGGGACCTGTCTAGCGAGGAGGAGATCCACCACTTTAAGAATGAGAGCTT
  AGGCATGGCCTTCCTGCACCTGTGTCACCTTGCTCTCAGCCGAGGCGTCCCCCTGGAGGAGATGGCCAGA
  GAGATCAGCTTCAAGAACTGCATCCCTCATTCCTTCCGACAGCACATCCGGCAGCACAATGTGCTCACAC
  GCCTGCGGCTCCACAGAGTCTTCCGCCGCTTCCTGCGGGCCTTCCGGCCTGGCCACCTCTCCCAGCAGGT
  TGTGATGGTGAAGTACTTGGCTACCCTGGAGCGGCTGGCTCCGCGCTTTGGCTCAGAGCGCATACCTGTG
  TGTCATCTGGAGGTCCTGGCACAGCCCGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGACCGCTG
  GGGATCCAGGCCCAGAGCTGCCTTCCGGGCCTCCCACCCACGAGGTACTGGTGACAGGCACCGGAGGTAT
  CCAGTGGCATCCGCTGCAGACCCAGGAATCTGAGAGAGGTAACAGCAGAGGGAATCCCCACGGCAGCCGG
  TCGGGGAAGAAGCCCAAAGCCCCCAAGGCTGGAGAGCACCTGACAGAGAGCCCTCAGGAACCACCTTGGA
  CCTACTTCTGTGACTTCCAGGACATTTCCCACGTGGTGCTAAAGGAGCGTCGCGTGCACATCCACCTCCA
  AGACAACAAGTGCTTGTTGCTGTGCCTCTGTTCCCAGGCTGAGGCCCTGTCCTTTGTGGCCCTGGTCGAT
  GGCTATTTCCGCTTGACTGCTGACTCCAGCCACTATCTGTGCCATGAGGTGGCACCCCCGAGGCTGGTGA
  CTAGCATCCAGAACGGCATCCATGGGCCCCTGATGGATCCGTTTGTACAGGCCAAGCTGTGGCCAGAGGA
  CGGCCTCTACCTGATTCAGTGGAGCACCAGCCACCTGCACCGCCTGATCCTTACCGTGGCCCATCGAAAC
  CCGGCTTTCAGTAATGGCCCTCGGGGCCTGCGCCTGCGAAAGTTCCCCATCACACAGCAGCCTGGAGCCT
  TTGTGCTGGATGGCTGGGGCCGCTCCTTTGCCAGCTTGGGGGACCTTCGGCTCGCCTTGCAGGGCTGCTC
  GTTGCGGGCCGGTGATGACTGTTTCCCCCTGCACCACTGCTGCCTGCCCCGGCCAAGAGAAATCTCCAAC
  CTCGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCTCTCAACCTCAGTCAGCTCAGCTTCCACAGGG
  TTCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGAGGGCCT
  TCTAAGAGTGGGAGGCCCCGATGAGGGCAAAGTGGACAATGGATGTCCCCCTGAGCCTGGTGGGACTAGT
  GGGCAGCAGCTTCGAGTGGTGCTCAAAGTTCTCGACCCCAGTCACCATGATATCGCCTTGGCCTTCTATG
  AGACTGCTAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTTTGCGTGCGTGG
  CTCAGAGAATATCATTGTGACAGAATTCGTAGAACATGGTCCCTTGGATGTGTGGTTACGGCGACAGAGG
  GGCCAAGTGCCCATGACCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTCAGCTACCTGGAGG
  ACAAGAATCTGGTTCACGGGAATGTATGTGGCCGGAACATCCTGCTGGCTCGCCTGGGGTTGGAGGAGGG
  TACCAACCCCTTCATCAAGCTAAGTGATCCTGGTGTGGGCCAGGGTGCCCTCTCCAGGGAAGAGCGGGTG
  GAGCGCATCCCCTGGACAGCTCCCGAGTGCCTGTCTGGAGGGACCAGTAGCTTGGGTACTGCCACGGACA
  TGTGGGGCTTTGGTGCCACCCTTCTTGAGATCTGCTTTGATGGGGAGGCACCCCTGCAGGGTCGTGGTCC
  CTCTGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCTGAACCCTCATCCCCAGAGCTGGCCACA
  CTCACCCGCCAGTGCCTGACCTATGAACCAGCACAGCGGCCATCCTTCCGCACCATTTTGCGGGACCTCA
  CAAGGCTGCAGCCACAGAATCTAGTCGGCACTTCGGCTGTGAACTCAGACTCACCAGCATCAGACCCCAC
  TGTTTTCCACAAGCGCTATTTGAAAAAGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTCAGCCTG
  TACTGCTACGACCCAACCAATGACGGCACTGGCGAGATGGTGGCCGTGAAGGCCCTGAAGGAAGGGTGCG
  GTCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAGATCCTGCGGACGCTGTACCACGAACATATTGT
  CAAGTATAAAGGCTGCTGTGAGGACCAAGGAGAGAAGTCTGTACAGCTGGTCATGGAGTACGTCCCTCTG
  GGCAGCCTCCGAGACTACCTGCCAAGGCACTGCGTAGGGCTGGCGCAGCTCCTGCTGTTTGCCCAGCAGA
  TCTGCGAGGGCATGGCCTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCCGCGCGCAACGTGCT
  GCTGGACAACGACAGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGCCACGAG
  TACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTGTTCTGGTATGCCCCAGAATGCCTGAAGGAGTGCA
  AATTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTAACTCTGTATGAGTTGTTGACATACTGTGACTC
  TAACCAGAGTCCCCATATGAAATTCACCGAGCTCATCGGCCATACCCAGGGCCAGATGACCGTGCTGAGG
  CTCACAGAGCTGCTGGAACGAGGAGAGAGGCTGCCTCGGCCTGACAGGTGTCCCTGTGAGATCTATCACC
  TCATGAAGAACTGCTGGGAGACAGAGGCCTCCTTCCGGCCCACCTTCCAGAATCTTGTGCCCATCCTCCA
  GACAGCACAGGAGAAGTACCAAGGTCAGGTGCCTTCCGTGTTCAGCGTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211764 representing NM_018793
  Red=Cloning site Green=Tags(s)
  
  MVGTMPLCGRRAILEDSKADGTEAQPLVPTGCLMVLLHWPGPEGGEPWVTFSQTSLTAEEVCIHIAHKVG
  ITPPCLNLFALYNAQAKVWLPPNHILDTSQDMNLYFRMRFYFRNWHGMNPQEPAVYRCGFPGAETSSDRA
  EQGVQLLDSASFEYLFEQGKHEFMNDVVSLRDLSSEEEIHHFKNESLGMAFLHLCHLALSRGVPLEEMAR
  EISFKNCIPHSFRQHIRQHNVLTRLRLHRVFRRFLRAFRPGHLSQQVVMVKYLATLERLAPRFGSERIPV
  CHLEVLAQPERDPCYIQNSGQTAGDPGPELPSGPPTHEVLVTGTGGIQWHPLQTQESERGNSRGNPHGSR
  SGKKPKAPKAGEHLTESPQEPPWTYFCDFQDISHVVLKERRVHIHLQDNKCLLLCLCSQAEALSFVALVD
  GYFRLTADSSHYLCHEVAPPRLVTSIQNGIHGPLMDPFVQAKLWPEDGLYLIQWSTSHLHRLILTVAHRN
  PAFSNGPRGLRLRKFPITQQPGAFVLDGWGRSFASLGDLRLALQGCSLRAGDDCFPLHHCCLPRPREISN
  LVIMRGSRAHTRPLNLSQLSFHRVHQDEITQLSHLGQGTRTNVYEGLLRVGGPDEGKVDNGCPPEPGGTS
  GQQLRVVLKVLDPSHHDIALAFYETASLMSQVSHMHLAFLHGVCVRGSENIIVTEFVEHGPLDVWLRRQR
  GQVPMTWKMVVAQQLASALSYLEDKNLVHGNVCGRNILLARLGLEEGTNPFIKLSDPGVGQGALSREERV
  ERIPWTAPECLSGGTSSLGTATDMWGFGATLLEICFDGEAPLQGRGPSEKERFYTKKHQLPEPSSPELAT
  LTRQCLTYEPAQRPSFRTILRDLTRLQPQNLVGTSAVNSDSPASDPTVFHKRYLKKIRDLGEGHFGKVSL
  YCYDPTNDGTGEMVAVKALKEGCGPQLRSGWQREIEILRTLYHEHIVKYKGCCEDQGEKSVQLVMEYVPL
  GSLRDYLPRHCVGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHE
  YYRVREDGDSPVFWYAPECLKECKFYYASDVWSFGVTLYELLTYCDSNQSPHMKFTELIGHTQGQMTVLR
  LTELLERGERLPRPDRCPCEIYHLMKNCWETEASFRPTFQNLVPILQTAQEKYQGQVPSVFSVC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018793
• ORF Size: 3552 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018793.2, NP_061263.2
• RefSeq Size: 4822 bp
• RefSeq ORF: 3555 bp
• Locus ID: 54721
• UniProt ID: Q9R117
• Cytogenetics: 9 A3
• MW: 133.8 kDa
• Gene Summary: Involved in intracellular signal transduction by amplifying type I and type II IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain. Plays an essential role in promoting selective immune responses, including innate host defense mechanisms and specific antiviral activities.[UniProtKB/Swiss-Prot Function]