Hip1r (NM_145070) Mouse Tagged ORF Clone

CAT#: MR211576

Hip1r (Myc-DDK-tagged) - Mouse huntingtin interacting protein 1 related (Hip1r)

Product Images

Circular map for MR211576

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hip1r
• Synonyms: AA410023; mKIAA0655
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211576 representing NM_145070
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCATCAAGAATGTGCCGGCGCGGGTGCTGAGCCGCAGGCCGGGCCACAGCCTAGAGGCCGAGC
  GCGAGCAGTTCGACAAGACGCAGGCCATCAGTATCAGCAAAGCCATCAACAGCCAGGAGGCCCCAGTGAA
  GGAGAAGCATGCCCGGCGTATCATCCTGGGCACGCATCATGAGAAGGGAGCCTTTACCTTCTGGTCCTAT
  GCCATCGGCCTGCCGCTGTCCAGCAGCTCCATCCTCAGCTGGAAGTTCTGTCACGTCCTTCACAAGGTCC
  TCCGGGACGGACACCCCAACGTCCTGCATGACTATCAGCGGTACCGGAGCAACATACGTGAGATCGGTGA
  CTTGTGGGGCCACCTTCGTGACCAGTATGGACACCTGGTGAATATCTATACCAAACTGTTGCTGACTAAG
  ATCTCCTTCCACCTTAAGCATCCCCAGTTTCCTGCAGGCCTGGAGGTAACAGATGAGGTGTTGGAGAAGG
  CGGCGGGAACTGATGTCAACAACATTTTTCAGCTTACCGTGGAGATGTTTGACTACATGGACTGTGAACT
  GAAGCTTTCTGAGTCAGTTTTCCGGCAGCTCAACACGGCCATCGCAGTGTCCCAGATGTCTTCTGGCCAG
  TGTCGCCTAGCGCCGCTCATCCAGGTCATTCAGGACTGCAGCCACCTGTACCACTACACAGTGAAGCTCA
  TGTTTAAGCTGCACTCCTGTCTCCCGGCAGACACCCTGCAAGGCCACAGGGATCGGTTCCACGAGCAGTT
  TCACAGCCTCAAAAACTTCTTCCGCCGAGCTTCAGACATGCTGTACTTCAAGAGGCTCATCCAGATCCCG
  CGGCTGCCTGAGGGACCCCCCAATTTCCTGCGGGCTTCAGCCCTGGCTGAGCACATCAAGCCGGTGGTGG
  TGATTCCCGAGGAGGCCCCAGAGGAAGAGGAGCCTGAGAACCTAATTGAAATCAGCAGTGCACCCCCTGC
  TGGGGAGCCAGTGGTGGTGGCTGACCTCTTTGATCAGACCTTTGGACCCCCCAATGGCTCCATGAAGGAT
  GACAGGGACCTCCAAATCGAGAACTTGAAGAGAGAGGTGGAGACCCTCCGTGCTGAGCTGGAGAAGATTA
  AGATGGAGGCACAGCGGTACATCTCCCAGCTGAAGGGCCAGGTGAATGGCCTGGAGGCAGAGCTGGAGGA
  GCAGCGCAAGCAGAAGCAGAAGGCCCTGGTGGACAACGAGCAGCTGCGCCACGAGCTGGCCCAGCTCAAG
  GCCCTGCAGCTGGAGGGCGCCCGCAACCAGGGCCTTCGAGAGGAAGCAGAGAGGAAGGCCAGTGCCACGG
  AGGCACGCTACAGCAAGCTGAAGGAGAAACACAGCGAACTCATTAACACCCACGCCGAGCTGCTCAGGAA
  GAACGCAGACACGGCCAAGCAGCTGACAGTGACACAGCAGAGCCAGGAGGAGGTGGCACGGGTAAAGGAA
  CAGCTGGCCTTCCAGATGGAGCAAGCGAAGCGTGAGTCTGAGATGAAGATGGAAGAGCAGAGCGACCAGT
  TGGAGAAGCTCAAGAGGGAGCTGGCGGCCAGGGCAGGAGAGCTGGCCCGTGCGCAGGAGGCCCTGAGCCG
  CACAGAACAGAGTGGGTCAGAGCTGAGCTCACGGCTGGACACACTGAATGCGGAGAAGGAAGCTCTGAGT
  GGAGTCGTTCGGCAGCGTGAGGCAGAGCTGCTGGCCGCTCAGAGCCTGGTGCGGGAGAAGGAGGAGGCAC
  TTAGCCAAGAGCAGCAGCGGAGCTCCCAGGAGAAGGGCGAGCTACGGGGGCAGCTGGCAGAAAAGGAGTC
  TCAGGAGCAGGGGCTTCGGCAGAAGCTGCTGGATGAGCAGTTGGCGGTGTTGCGAAGTGCAGCCGCCGAG
  GCAGAGGCCATCCTACAGGATGCAGTGAGCAAGCTGGACGACCCCCTGCACCTCCGCTGCACCAGCTCCC
  CAGACTACTTGGTGAGCCGGGCTCAGGCAGCCCTGGACAGCGTGAGCGGCCTGGAGCAGGGCCACACCCA
  GTACCTGGCTTCCTCTGAAGATGCTTCTGCCCTGGTGGCAGCGCTGACCCGCTTCTCCCATTTGGCTGCG
  GACACCATTGTCAATGGTGCCGCCACCTCCCACCTGGCCCCCACCGACCCCGCCGACCGCCTGATGGACA
  CATGCAGGGAGTGTGGAGCCCGGGCTCTGGAGCTGGTGGGACAGCTGCAAGACCAGACAGTGCTACCGAG
  GGCTCAGCCCAGCCTGATGCGGGCCCCCCTGCAGGGCATTCTGCAGTTGGGCCAGGACTTGAAGCCTAAG
  AGCCTGGATGTACGGCAAGAGGAGCTAGGGGCCATGGTGGACAAGGAGATGGCGGCCACCTCGGCAGCCA
  TTGAGGACGCTGTGCGGAGGATCGAGGACATGATGAGCCAGGCCCGCCACGAGAGCTCGGGCGTGAAACT
  GGAGGTGAATGAGAGGATCCTCAACTCCTGCACAGACCTGATGAAGGCTATCCGGCTCCTGGTGATGACC
  TCCACCAGCCTGCAGAAGGAAATTGTGGAGAGCGGCAGGGGGGCAGCAACGCAGCAGGAATTTTATGCCA
  AGAATTCACGGTGGACTGAAGGCCTCATCTCAGCCTCTAAGGCAGTGGGCTGGGGAGCCACACAGCTGGT
  GGAGTCAGCTGACAAGGTTGTGCTTCACATGGGCAAATACGAGGAACTCATCGTCTGCTCCCATGAGATT
  GCGGCCAGCACGGCCCAGCTGGTGGCAGCCTCGAAGGTGAAAGCCAACAAGAACAGTCCCCACTTGAGCC
  GCCTGCAGGAATGTTCCCGCACTGTCAACGAGAGGGCTGCCAACGTCGTGGCCTCCACCAAATCTGGCCA
  GGAGCAGATTGAGGACAGAGACACCATGGATTTCTCTGGCCTGTCCCTCATCAAGTTGAAGAAGCAGGAG
  ATGGAGACACAGGTGCGAGTCTTGGAGCTGGAGAAGACACTAGAGGCAGAGCGTGTCCGGCTCGGGGAGC
  TTCGGAAACAGCACTATGTACTGGCTGGGGGGATGGGAACACCTAGCGAAGAAGAACCCAGCAGACCCAG
  CCCAGCTCCCCGAAGTGGGGCCACTAAGAAGCCACCGCTGGCCCAGAAACCCAGCATAGCCCCCAGGACA
  GACAACCAGCTCGACAAAAAGGATGGTGTCTACCCAGCTCAACTTGTGAACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211576 representing NM_145070
  Red=Cloning site Green=Tags(s)
  
  MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINSQEAPVKEKHARRIILGTHHEKGAFTFWSY
  AIGLPLSSSSILSWKFCHVLHKVLRDGHPNVLHDYQRYRSNIREIGDLWGHLRDQYGHLVNIYTKLLLTK
  ISFHLKHPQFPAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTAIAVSQMSSGQ
  CRLAPLIQVIQDCSHLYHYTVKLMFKLHSCLPADTLQGHRDRFHEQFHSLKNFFRRASDMLYFKRLIQIP
  RLPEGPPNFLRASALAEHIKPVVVIPEEAPEEEEPENLIEISSAPPAGEPVVVADLFDQTFGPPNGSMKD
  DRDLQIENLKREVETLRAELEKIKMEAQRYISQLKGQVNGLEAELEEQRKQKQKALVDNEQLRHELAQLK
  ALQLEGARNQGLREEAERKASATEARYSKLKEKHSELINTHAELLRKNADTAKQLTVTQQSQEEVARVKE
  QLAFQMEQAKRESEMKMEEQSDQLEKLKRELAARAGELARAQEALSRTEQSGSELSSRLDTLNAEKEALS
  GVVRQREAELLAAQSLVREKEEALSQEQQRSSQEKGELRGQLAEKESQEQGLRQKLLDEQLAVLRSAAAE
  AEAILQDAVSKLDDPLHLRCTSSPDYLVSRAQAALDSVSGLEQGHTQYLASSEDASALVAALTRFSHLAA
  DTIVNGAATSHLAPTDPADRLMDTCRECGARALELVGQLQDQTVLPRAQPSLMRAPLQGILQLGQDLKPK
  SLDVRQEELGAMVDKEMAATSAAIEDAVRRIEDMMSQARHESSGVKLEVNERILNSCTDLMKAIRLLVMT
  STSLQKEIVESGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVESADKVVLHMGKYEELIVCSHEI
  AASTAQLVAASKVKANKNSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSGLSLIKLKKQE
  METQVRVLELEKTLEAERVRLGELRKQHYVLAGGMGTPSEEEPSRPSPAPRSGATKKPPLAQKPSIAPRT
  DNQLDKKDGVYPAQLVNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145070
• ORF Size: 3204 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145070.3, NP_659507.3
• RefSeq Size: 4402 bp
• RefSeq ORF: 3207 bp
• Locus ID: 29816
• UniProt ID: Q9JKY5
• Cytogenetics: 5 F
• MW: 119.4 kDa
• Gene Summary: Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.[UniProtKB/Swiss-Prot Function]