Herc4 (NM_026101) Mouse Tagged ORF Clone

CAT#: MR211530

Herc4 (Myc-DDK-tagged) - Mouse hect domain and RLD 4 (Herc4), transcript variant 2

Product Images

Circular map for MR211530

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Herc4
• Synonyms: 1700056O17Rik; 4921531D01Rik; 9530080M15Rik; mKIAA1593
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211530 representing NM_026101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGCTGGGGCAATGCATCCTATGGACAACTAGGTTTGGGAGGAATTGATGAAGAAATTGTACTAG
  AGCCCAGGAGAAGTGACTTTTTCGTGAACAAAAAGGTCCGAGATGTAGGCTGTGGACTCAGGCACACTGT
  GTTTGTCCTGGATGATGGGACTGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAG
  TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAGAATATCGTAGCTGTTGCGTGTGGAGAAG
  CTCACACGTTAGCGCTGAATGACAAGGGCCAGGTGTATGCTTGGGGTCTCGACTCTGACGGACAGCTTGG
  CCTACAGGGATCAGAGGAATGTATCAGAGTACCCAGAAATATTAAAAGTCTTTCGGATATCCAGATAGTA
  CAGGTTGCGTGTGGTTACTATCATTCGCTTGCACTTTCTAAAGCAAGTGAAGTTTTCTGTTGGGGACAGA
  ATAAATATGGCCAGCTGGGTCTAGGCATTGATTGTCAAAAGCAAACTTCACCACAGCTGATTAAGTCTTT
  GCTTGGAATACCATTCATGCAAGTCGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCCGGAGCT
  ATCTTTGGATGGGGACGTAACAAATTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA
  ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGCGGAGAAGATCATACTGCTGCATT
  AACCAAGGAAGGTGGAGTGTTTACCTTTGGAGCTGGGGGCTATGGCCAGCTGGGTCATAATTCTACCAGT
  CATGAGATAAATCCCAGGAAAGTTTTTGAACTCATGGGAAGCATTGTTACTCAGGTCGCTTGTGGAAGGC
  AGCATACCTCTGCATTTGTTCCTTCTTCAGGACGAATTTACTCCTTTGGTCTTGGTGGTAATGGGCAGTT
  GGGAACTGGTTCAACCAGCAACAGAAAAAGCCCTTTCACTGTAAAAGGAAACTGGTTTTCTTATAATGGA
  CAGTGTCCACAAGATATTGGTTCTGAAGACTATTTCTGTGTCAAAAGAATTTTCTCAGGTGGAGATCAAA
  GCTTTTCACATTACTCTAGTCCTCAGAACTGTGGACCTCCAGATGACTTTAGATGCTCCGACCCTTCCAA
  GCAGATCTGGACAGTGAACGAAGCTCTGATTCAGAAATGGCTCAGCTACCCCTCTGGAAGGTTCCCTGTG
  GAGATAGCCAATGAGATAGATGGAACATTTTCTTCATCTGGCTGCCTAAATGGAAGTTTTCTGGCTATTA
  GCAATGACGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAACGCTGCTAGACTCTTATT
  CCACAAACTTATACAACCTGATCATCCTCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTA
  ATCCCTAAACTGACTAGTTCCCTACCTGATGTTGAAGCTCTGAGGTTTTATCTTACCCTGCCAGAGTGTC
  CCCTGATGAGTGACTGCAACAATTTCACAACAATAGCAATCCCCTTTGGTACAGCTCTTGTGAACCTAGA
  AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA
  GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGCATTCCCCCTTCTGAAAGAA
  GAATTTTCAACAGTTTTCTTCATACCGCATTAAAGGTTTTAGAAATATTGCATAGGGTAAATGAGAAAAC
  AGGACAACTTATTCAATATGACAAGTTTTACATCCATGAAGTCCAAGAGCTGATAGACATAAGGAATGAC
  TATATCAACTGGGTCCAGCAGCAAGCCTATGGAGTGTTGGCAGATATCCCGGTGACAATTTGTACATATC
  CATTTGTATTTGATGCCCAGGCAAAAACTACTCTGCTCCAGACAGATGCAGTCTTGCAGATGCAGATGGC
  CATTGACCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATCGAGTCTGTGAATCCTTGC
  TTAATTCTAGTTGTTCGCAGAGAGAATATTGTAGGAGATGCGATGGAAGTCCTCAGGAAAACCAAGAATA
  TAGATTATAAAAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTTGATGCAGGAGGTGTTCGCAA
  AGAATTTTTCTTGCTTATCATGAGGGAATTGTTGGATCCTAAATACGGCATGTTTCGATATTATGAAGAT
  TCCAGGCTCATTTGGTTTTCAGATAAGACATTTGAAGACAGTGATTTGTTCCACTTGATCGGTGTTATCT
  GTGGATTAGCAATTTATAATTTTACCATTGTGGACCTCCATTTCCCTTTGGCTTTATATAAGAAACTGCT
  GAAAAGGAAGCCGTCCCTGGATGATCTGAAAGAGCTGATGCCAGCCGTCGGGAGAAGCATGCAGCAATTG
  CTGGACTATCCAGAAGATGACATAGAGGAAACATTTTGTCTAAACTTTACGATCACAGTTGAGAACTTCG
  GTGCAACAGAAGTAAAAGAGCTGGTTCTGAACGGGGCAGACACTGCTGTTAATAGACAGAATCGGCAGGA
  GTTTGTTGATGCCTATGTGGATTACATATTCAATAAATCAGTGGCATCTTTATTTGATGCTTTCCATGCG
  GGCTTTCATAAGGTCTGTGGAGGAAAAGTTCTTCTGCTCTTCCAGCCTAATGAATTACAAGCTATGGTTA
  TTGGGAATACAAATTATGATTGGAAAGAACTGGAGAAGAATACAGAATACAAAGGAGAGTATTGGGCAGA
  CCATCCCACGATAAAAATTTTTTGGGAAGTGTTTCATGAATTACCACTAGAGAAGAAGAAACAATTTCTA
  TTATTTTTGACAGGTAGTGATCGGATTCCCATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCAA
  CAGGAGGTGGTGAGAGCTATCTCCCGGTTTCCCATACCTGTTTTAATCTTCTAGATCTCCCAAAATATAC
  AGAGAAAGAAACCCTCCGATGTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211530 representing NM_026101
  Red=Cloning site Green=Tags(s)
  
  MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEK
  SRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEECIRVPRNIKSLSDIQIV
  QVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGA
  IFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTS
  HEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNG
  QCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCSDPSKQIWTVNEALIQKWLSYPSGRFPV
  EIANEIDGTFSSSGCLNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNL
  IPKLTSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIV
  ELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRND
  YINWVQQQAYGVLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPC
  LILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYED
  SRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQL
  LDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHA
  GFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFL
  LFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026101
• ORF Size: 3147 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026101.4, NP_080377.2
• RefSeq Size: 4019 bp
• RefSeq ORF: 3150 bp
• Locus ID: 67345
• UniProt ID: Q6PAV2
• Cytogenetics: 10 B4
• MW: 118 kDa
• Gene Summary: Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.[UniProtKB/Swiss-Prot Function]