Itga4 (NM_010576) Mouse Tagged ORF Clone

CAT#: MR211489

Itga4 (Myc-DDK-tagged) - Mouse integrin alpha 4 (Itga4)

Product Images

Circular map for MR211489

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga4
• Synonyms: CD49D; Itga4B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211489 representing NM_010576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGAAGCGAGGTGCAGACCGAGGTCCCGAGGGATCGCCCTCCGGGAAGCGGTGATGCTGTTGT
  TGTACTTCGGGGTGCCAACCGGGCACTCCTACAACCTGGACCCGGAGAATGCACTGCTGTACCAGGGCCC
  CTCCGGCACGCTGTTTGGCTACTCGGTGGTGCTGCACAGCCACGGGTCGAAGCGCTGGCTCATCGTGGGG
  GCTCCCACTGCCAGCTGGCTCTCTAATGCCTCAGTGGTCAATCCTGGGGCGATTTACAGATGCGGGATCA
  GAAAGAATCCAAACCAGACCTGCGAACAGCTCCAGCTGGGTAGCCCCAGTGGAGAGCCTTGTGGGAAGAC
  ATGCCTGGAGGAGAGGGATAACCAGTGGCTGGGGGTCACCCTTTCCAGACAGCCTGGAGAAAATGGCTCT
  ATCGTGACTTGTGGGCACAGGTGGAAAAATATTTTTTACATGAAGAGCGATAACAAACTCCCCACTGGCA
  TTTGCTACGTCATGCCTTCTGATTTGCGGACAGAACTGAGTAAAAGGATGGCCCCGTGTTACAAAGATTA
  TACGAGAAAATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCTAGTTTTTACACACAGGATTTA
  ATTGTGATGGGGGCCCCGGGATCATCGTACTGGACTGGCACCGTCTTTGTCTACAATATAACTACAAACC
  AATACAAAGCATTTGTAGACAGACAGAACCAAGTAAAATTTGGAAGCTACTTAGGCTACTCAGTTGGAGC
  TGGACATTTTCGAAGTCCACATACTACCGAAGTCGTGGGAGGAGCCCCTCAACACGAACAGATAGGAAAA
  GCATATATATTTAGCATTGATGAAAACGAACTGAACATCGTATATGAAATGAAAGGTAAAAAGCTTGGCT
  CATACTTTGGAGCTTCTGTCTGCGCTGTGGACCTCAATGCAGATGGCTTCTCAGATCTCCTTGTTGGAGC
  TCCCATGCAGAGCACCATCAGGGAGGAAGGAAGAGTATTCGTGTACATCAACTCTGGCATGGGAGCTGTG
  ATGGTTGAAATGGAAAGGGTCCTTGTCGGAAGTGACAAATATGCTGCAAGATTTGGGGAGTCTATAGCGA
  ATCTTGGCGACATTGACAATGACGGCTTTGAAGATATTGCTATTGGTGCACCACAAGAAGACGACTTGAG
  AGGTGCTGTCTACATTTACAATGGCCGAGTCGATGGAATCTCCTCCACCTACTCACAGAGAATTGAAGGA
  CAGCAAATCAGCAAATCATTAAGGATGTTTGGACAATCTATCTCAGGACAAATTGATGCAGACAACAATG
  GATATGTTGATGTAGCCGTTGGTGCATTTCAATCTGATTCTGCAGTGTTGCTAAGGACAAGGCCTGTAGT
  GATTGTTGAAGCATCTTTAAGCCATCCTGAGTCTGTAAATAGGACAAAGTTTGACTGTACTGAAAATGGA
  CTTCCATCTGTGTGCATGCATCTTACACTGTGTTTCTCATATAAAGGCAAAGAGGTCCCAGGCTACATCG
  TTTTGTTTTACAATGTGAGCTTGGATGTGCACAGGAAGGCAGAGTCTCCGTCAAGATTTTATTTCTTCTC
  TAATGGGACTTCTGACGTGATTACAGGAAGCATACGAGTTTCAAGCAGTGGAGAGAAATGTAGGACACAC
  CAGGCATTCATGCGGAAAGACGTGCGAGACATCCTTACCCCCATTCATGTAGAGGCCACATACCACCTTG
  GGCATCATGTGATCACCAAACGAAACACTGAGGAATTTCCACCACTCCAGCCGATCCTTCAGCAGAAGAA
  AGAAAAAGACGTTATTAGAAAAATGATAAACTTTGCAAGGTTTTGTGCCTATGAAAATTGCTCTGCTGAT
  CTCCAAGTTTCTGCAAAAGTTGGATTTTTGAAGCCATATGAAAATAAAACCTATCTTGCTGTTGGGAGCA
  TGAAGACCATAATGCTAAACGTGTCCTTGTTCAACGCTGGCGATGATGCTTACGAAACCACTCTGAATGT
  CCAACTCCCCACAGGCCTTTATTTCATTAAGATCTTAGACCTGGAAGAGAAACAAATAAACTGCGAAGTG
  ACTGAGAGCTCAGGCATAGTGAAGCTTGCCTGCAGCCTAGGTTACATATATGTGGATCGCCTCTCAAGGA
  TAGACATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGGGCACATGAGGACCTCAGCATCAGTGTGCA
  TGCCTCCTGTGAAAACGAGGGCGAATTGGACCAAGTGAGGGACAACAGAGTGACCTTAACGATACCTCTA
  AGGTATGAGGTTATGCTGACTGTTCATGGGCTTGTGAACCCAACTTCATTTGTGTATGGATCTAGCGAAG
  AAAACGAGCCAGAAACATGCATGGCCGAGAAGCTGAACCTCACTTTCCATGTTATAAACACTGGGATTAG
  CATGGCTCCAAATGTTAGTGTGAAAATAATGGTACCAAATTCTTTTCTCCCTCAAGATGATAAGTTGTTC
  AACGTTTTGGATGTCCAGACAACTACAGGGCAATGCCATTTTAAACACTATGGAAGAGAGTGTACATTTG
  CACAGCAAAAAGGCATAGCGGGGACGTTGACCGATATAGTCAAATTCCTATCAAAGACTGATAAGAGACT
  CCTGTATTGCATGAAAGCTGATCAACACTGTTTAGATTTCTTATGCAATTTCGGAAAAATGGAAAGTGGG
  AAGGAAGCCAGCGTTCATATTCAGCTGGAGGGCAGGCCATCCATCTTGGAAATGGATGAGACCTCATCAC
  TCAAGTTTGAAATAAAAGCAACAGCTTTTCCAGAGCCACACCCAAAAGTTATTGAACTAAATAAAGATGA
  GAACGTGGCCCATGTTTTCTTGGAAGGGCTCCATCATCAAAGACCCAAACGACATTTCACCATCATTATT
  ATTACCATCAGCTTGCTACTTGGACTTATTGTACTTTTATTAATTTCATGTGTTATGTGGAAGGCTGGAT
  TCTTTAAAAGACAGTACAAATCTATCCTACAAGAAGAAAACAGGAGAGACAGCTGGAGTTATGTCAACAG
  CAAAAGCAATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211489 representing NM_010576
  Red=Cloning site Green=Tags(s)
  
  MAAEARCRPRSRGIALREAVMLLLYFGVPTGHSYNLDPENALLYQGPSGTLFGYSVVLHSHGSKRWLIVG
  APTASWLSNASVVNPGAIYRCGIRKNPNQTCEQLQLGSPSGEPCGKTCLEERDNQWLGVTLSRQPGENGS
  IVTCGHRWKNIFYMKSDNKLPTGICYVMPSDLRTELSKRMAPCYKDYTRKFGENFASCQAGISSFYTQDL
  IVMGAPGSSYWTGTVFVYNITTNQYKAFVDRQNQVKFGSYLGYSVGAGHFRSPHTTEVVGGAPQHEQIGK
  AYIFSIDENELNIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGMGAV
  MVEMERVLVGSDKYAARFGESIANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQRIEG
  QQISKSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDCTENG
  LPSVCMHLTLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDVITGSIRVSSSGEKCRTH
  QAFMRKDVRDILTPIHVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDVIRKMINFARFCAYENCSAD
  LQVSAKVGFLKPYENKTYLAVGSMKTIMLNVSLFNAGDDAYETTLNVQLPTGLYFIKILDLEEKQINCEV
  TESSGIVKLACSLGYIYVDRLSRIDISFLLDVSSLSRAHEDLSISVHASCENEGELDQVRDNRVTLTIPL
  RYEVMLTVHGLVNPTSFVYGSSEENEPETCMAEKLNLTFHVINTGISMAPNVSVKIMVPNSFLPQDDKLF
  NVLDVQTTTGQCHFKHYGRECTFAQQKGIAGTLTDIVKFLSKTDKRLLYCMKADQHCLDFLCNFGKMESG
  KEASVHIQLEGRPSILEMDETSSLKFEIKATAFPEPHPKVIELNKDENVAHVFLEGLHHQRPKRHFTIII
  ITISLLLGLIVLLLISCVMWKAGFFKRQYKSILQEENRRDSWSYVNSKSNDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010576
• ORF Size: 3096 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010576.4
• RefSeq Size: 9833 bp
• RefSeq ORF: 3099 bp
• Locus ID: 16401
• UniProt ID: Q00651
• Cytogenetics: 2 47.38 cM
• MW: 115.5 kDa
• Gene Summary: Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.[UniProtKB/Swiss-Prot Function]