Nat10 (NM_153126) Mouse Tagged ORF Clone

CAT#: MR211470

Nat10 (Myc-DDK-tagged) - Mouse N-acetyltransferase 10 (Nat10)

Product Images

Circular map for MR211470

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nat10
• Synonyms: AI429152
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211470 representing NM_153126
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCGGAAGAAGGTGGATAACCGAATTCGAATTCTCATTGAGAATGGCGTAGCTGAGCGGCAGAGGT
  CTCTTTTTGTTGTAGTTGGGGATCGAGGAAAAGATCAGGTGGTTATTCTTCATCATATGTTGTCCAAGGC
  AACTGTGAAGGCTCGGCCCTCAGTCCTGTGGTGTTATAAGAAAGAGCTGGGATTTAGCAGTCACCGGAAG
  AAGAGGATGCGGCAGCTACAGAAGAAAATAAAGAGTGGGACCTTGAACTTAAAGCAAGATGACCCCTTTG
  AGCTTTTTGTAGCAGCCACAAACATTCGCTACTGCTACTACAATGAAACCCACAAGATTCTGGGCAATAC
  TTTCGGCATGTGTGTCCTCCAGGATTTTGAAGCGTTAACTCCGAACTTGTTGGCCAGAACTGTAGAAACA
  GTAGAAGGTGGTGGACTGGTGGTCATCCTCCTGCGGACCATGAACTCGCTTAAGCAGCTGTACACGATGA
  CTATGGATGTGCATTCCAGGTACAGGACTGAGGCCCATCAGGACGTGGTGGGAAGATTTAACGAGAGGTT
  TATTCTCTCTCTGGCCTCTTGTAAGAAGTGTCTGGTCATTGACGATCAGCTCGACATCCTGCCCATCTCC
  TCCCACGTGGCCAGCATTGAAGCCTTACCTCCTCAGGCCCCGGATGAGAATCTCAGTCCTGCTGCTCTGG
  AGCTGCTGGAGTTGAAAGAGAGCTTGCAGGACACTCAGCCCGTGGGTGTTCTGGTGGACTGCTGCAAGAC
  CCTGGACCAGGCCAAAGCTGTCTTGAAATTCATTGAGGGGATCTCGGAGAAGACTCTAAGGAGTACTGTA
  GCCCTCACCGCTGCCCGAGGAAGGGGCAAGTCTGCGGCCCTGGGCCTGGCTATCGCTGGAGCAGTGGCAT
  TCGGGTATTCCAATATTTTTGTTACCTCCCCAAGCCCGGATAACCTCCACACGCTGTTTGAATTTGTATT
  TAAAGGATTTGATGCTCTGCAGTATCAGGAGCATCTGGATTATGAGATTGTACAGTCGCTGAACCCCGAG
  TTTAATAAAGCGGTGATCAGGGTCAATGTGTTCCGAGAGCACAGACAGACTATTCAGTACATCCACCCTG
  CAGATGCTGTGAAACTGGGCCAGGCTGAGCTGGTTGTGATAGATGAAGCTGCCGCTATTCCCCTCCCCCT
  GGTGAAGAGCCTGCTTGGGCCCTACCTGGTTTTCATGGCATCTACTATCAATGGCTACGAGGGCACTGGC
  CGGTCACTGTCCCTCAAGCTCATTCAACAACTCCGTCAGCAGAGTGCCCAGAGCCAGGTCAGCACCACTG
  CTGAGAACAAGACCACAACGACAGCCAGACTGGCCTCAGCTCGAACCTTGCATGAGGTTTCCCTCCAAGA
  GTCAATCCGATACGCCCCTGGGGATGCAGTGGAGAAGTGGCTTAATGACCTGCTGTGCCTGGATTGCCTC
  AACATCACCCGCATCGTTTCCGGCTGCCCCTTGCCTGAGGCCTGTGAGCTCTACTATGTTAACAGAGATA
  CCCTCTTTTGCTACCACAAGGCCTCTGAAGTTTTCCTCCAGCGGCTCATGGCTCTCTATGTGGCTTCTCA
  TTACAAGAACTCTCCCAACGACCTGCAGATGCTCTCAGACGCTCCTGCTCACCACCTCTTCTGCCTCCTG
  CCACCTGTGCCCCCCACCCAGAATGCCCTGCCTGAAGTGCTTGCAGTTGTCCAGGTGTGCCTTGAGGGAG
  AGATTTCTCGTCAGTCCATTTTGAATAGCCTCTCTCGAGGCAAGAAGGCTTCTGGGGACCTGATTCCATG
  GACAGTGTCGGAACAGTTTCAAGATCCAGACTTTGGAGGCCTTTCTGGTGGACGGGTTGTTCGAATTGCT
  GTTCACCCAGATTATCAAGGGATGGGATATGGCAGCCGAGCGCTGCAGCTGCTGCAGATGTATTATGAAG
  GCAAGTTCCCTTGTCTGGAGGAAAAAGTCTTGGAGACACCACAAGAAATCCGCACCGTGAGCAGTGAGGC
  TGTCAGCTTGTTGGAAGAAGTTATTACTCCCCGGAAGGATCTGCCCCCCTTGCTCCTCAAGTTGAATGAG
  AGGCCTGCAGAGCGCCTGGATTACCTGGGGGTTTCCTATGGGCTGACCCCCAGGCTTCTCAAGTTCTGGA
  AACGAGCTGGATTTGTTCCTGTCTATTTGAGACAGACCCCAAACGACCTGACTGGGGAACACTCGTGCAT
  TATGCTGAAGACGCTGGCCGATGAGGATGAGGCTGAGCAGGGAGCTTGGCTGGCAGCATTTTGGAAAGAT
  TTCCGAAGACGGTTCCTGGCCTTGCTGTCATACCAGTTCAGCACCTTCTCTCCTGCCCTGTCTCTGAACA
  TCATTCAGAACAGGAACGTAGCCAAGTCAGCCCTGCCAGCCCTAGGCCGGGAGCATCTGGAGGCACTTTT
  CCTTCCCTATGACCTGAAGCGTCTAGAGATGTACTCTCGGAACATGGTCGATTACCACCTCATCATGGAT
  CTTATCCCAGCCATTTCCCGCTTGTACTTCCTGAACCAGCTAGGAGACCTGTCCCTGTCGGCTGCCCAGT
  CGGCTCTTCTCTTGGGAATTGGCCTACAGCACAAGTCTGTGGATCAGCTGGAAAAGGAGATTGAGCTGCC
  CTCAGGCCAGTTGATGGGGCTTTTCAACCGGATCATCCGCAAAGTTGTGAAGCTGTTCAATGATGTGCAG
  GAAAAGGCTATTGAGGAGCAGATGGTGGCAGTGAAGGATGTGGTCATGGAGCCCACTATGAAGACCCTGA
  GTGATGACCTGGATGAAGCAGCAAAGGAATTCCAGGAGAAACACAAGAAGGAAGTCGGGAAGCTGAAGGA
  CATGGACCTCTCTCAATATGTAATTCGTGGGGACGATGAAGAGTGGAATGAAGTTTTGAGCAAAGCAGGG
  CAGAATGCCTCCATTGTTAGTTTGAAAAGTGACAAGAAAAGGAAACTGGAAACAAAACAAGAACCCAAAC
  AGAGCAAGAAGTTGAAGAAGAGAGATAACAACAGAAAGGATATGAAACTGAAGCGAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211470 representing NM_153126
  Red=Cloning site Green=Tags(s)
  
  MNRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK
  KRMRQLQKKIKSGTLNLKQDDPFELFVAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET
  VEGGGLVVILLRTMNSLKQLYTMTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLDILPIS
  SHVASIEALPPQAPDENLSPAALELLELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV
  ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIVQSLNPE
  FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG
  RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL
  NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL
  PPVPPTQNALPEVLAVVQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA
  VHPDYQGMGYGSRALQLLQMYYEGKFPCLEEKVLETPQEIRTVSSEAVSLLEEVITPRKDLPPLLLKLNE
  RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLADEDEAEQGAWLAAFWKD
  FRRRFLALLSYQFSTFSPALSLNIIQNRNVAKSALPALGREHLEALFLPYDLKRLEMYSRNMVDYHLIMD
  LIPAISRLYFLNQLGDLSLSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNDVQ
  EKAIEEQMVAVKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKDMDLSQYVIRGDDEEWNEVLSKAG
  QNASIVSLKSDKKRKLETKQEPKQSKKLKKRDNNRKDMKLKRKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153126
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153126.3
• RefSeq Size: 3856 bp
• RefSeq ORF: 3075 bp
• Locus ID: 98956
• UniProt ID: Q8K224
• Cytogenetics: 2 E2
• MW: 115.9 kDa
• Gene Summary: RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA (By similarity). May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity). Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (By similarity). Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity). Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation. In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro. The relevance of the protein lysine acetyltransferase activity is however unsure in vivo. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization (By similarity).[UniProtKB/Swiss-Prot Function]