Ipo13 (NM_146152) Mouse Tagged ORF Clone

CAT#: MR211326

Ipo13 (Myc-DDK-tagged) - Mouse importin 13 (Ipo13)

Product Images

Circular map for MR211326

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ipo13
• Synonyms: Imp13; Kap13; Ranbp13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211326 representing NM_146152
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGGGAGGAGCAGCTGGGGGCTGCAGGGGCTGGAGCAGCACCAGCCTTGGACTTCACTGTGG
  AGAACGTGGAGAAGGCGCTGCACCAGCTCTACTACGACCCCAATATTGACAACAAGAACCTGGCTCAGAA
  GTGGCTGATGCAGGCCCAGGTCTCTCCACAGGCCTGGCACTTCAGCTGGCAGCTACTACAGCCAGACAAG
  GTGCCCGAGATCCAGTACTTTGGGGCCAGTGCCCTGCACATCAAGATCTCTCGCTACTGGAGCGACATCC
  CCACCGACCAGTATGAAAGCTTAAAGGCACAGCTCTTCACCCAGATCACCCGCTTTGCCAGCGGCTCCAA
  AATTGTGCTGACTCGGCTGTGCGTGGCGCTGGCCTCACTAGCTCTCAGCATGATGCCTGACGCTTGGCCA
  TGTGCTGTGGCAGATATGGTTCGGCTCTTCCAGGCTGAGGACTCGCCGGTGGATAGCCAGGGCCGTTGCC
  TGGCCCTGCTAGAGTTGCTGACAGTACTGCCCGAGGAGTTTCAGACCAGCCGCTTGCCCCAGTACCGCAA
  AGGTCTGGTGCGGACCAGCCTGGCTGTGGAGTGTGGGACTGTCTTCCCATTGCTGGAGCAACTGCTACAA
  CAGCCCAGCTCTCCTAGCTGTGTGCGTCAGAAGGTGCTCAAGTGCTTCTCCAGTTGGGTGCAGCTGGAGG
  TACCCCTGCAGGACTGTGAGGCGCTCATTCAGGCTGCCTTCGCCGCTCTGCAGGACTCAGAGCTCTTTGA
  CAGCAGCGTGGAGGCCATTGTCAATGCCATCTCCCAACCTGATGCCCAAAGGTACGTGAACACACTCCTG
  AAACTCATTCCTCTGGTGCTGGGACTGCAGGAACAACTGCGGCAGGCAGTGCAGAATGGGGACATGGAGA
  CCTCCCATGGCATCTGCCGTATTGCTGTGGCCCTGGGCGAGAACCACTCTCGGGCCTTGCTAGACCAAGT
  AGAGCACTGGCAGAGCTTCCTGGCACTCGTCAACATGATCATGTTCTGCACTGGCATCCCTGGCCACTAT
  CCTGTCAACGAGACCACCAGCTCCCTCACTCTCACTTTCTGGTACACACTGCAGGATGACATTCTGTCCT
  TTGAGGCAGAGAAGCAGGCTGTGTACCAGCAGGTGTACCGGCCAGTCTACTTCCAGCTGGTGGACGTGCT
  TCTGCACAAGGCCCAGTTCCCTTCTGACGAGGAGTATGGATTCTGGTCCTCAGACGAAAAGGAGCAGTTC
  CGTATCTACAGGGTGGACATCTCAGACACACTCATGTATGTCTATGAGATGCTGGGGGCTGAGCTGCTTA
  GCAACCTCTATGACAAGCTGGGCCGTTTGCTCACCAGCTCAGAGGAGCCCTACTCCTGGCAGCACACAGA
  AGCCCTGCTCTATGGCTTCCAGTCCATTGCAGAGACCATCGATGTCAACTACTCTGATGTGGTACCAGGG
  CTCATTGGCCTCATCCCACGGATCAGCATCAGCAACGTGCAGTTGGCGGATACTGTCATGTTCACCATTG
  GAGCTCTGTCTGAATGGCTGGCTGACCACCCCGTCATGATCAACAGCGTTCTGCCCCTTGTGCTGCATGC
  CCTAGGCAATCCTGAGCTGTCTGTGTCATCTGTGTCGACCCTCAAAAAGATCTGCCGAGAGTGCAAGTAT
  GACCTGCCGCCCTATGCTGCCAACATTGTAGCTGTCTCCCAGGATGTGCTGATGAAGCAGATTCACAAGA
  CAAGCCAGTGCATGTGGCTGATGCAGGCCCTGGGCTTCCTGCTGTCAGCCCTGCAGGTGGAGGAGATCCT
  GAAGAACCTGCACTCCCTCATCTCTCCCTACATCCAACAGCTGGAGAAGCTGGCAGAAGAGATACCTAAT
  CCCTCTAACAAGCTGGCCATTGTCCACATCTTGGGGCTTCTTTCCAACCTCTTTACCACACTGGACGTCA
  GTCATCATGAGGATGATCATGAAGGCCCTGAACTCCGGAAGTTGCCAGTGCCACAAGGACCCAACCCGGT
  GGTGGTTGTGCTGCAGCAGGTCTTCCAGCTCATCCAGAAGGTGCTGAGCAAGTGGCTGAATGACGCCCAG
  GTGGTTGAGGCGGTATGCGCCATCTTTGAGAAGTCTGTTAAGACACTGCTGGATGACTTTGCCCCCATGG
  TGCCGCAGCTCTGTGAGATGCTGGGTCGGATGTACAGTACTGTTCCCCAGGCCTCTGCTCTTGACCTCAC
  CCGGCAGCTGGTCCACATCTTTGCCCACGAGCCTGCCCACTTTCCCCCAATAGAGGCCCTCTTCCTGCTG
  GTGACTTCCGTCACACTCAGTCTCTTCCAGCAAGGGCCCAGGGATCATCCTGATATTGTTGATTCATTTA
  TGCAACTCCTGGCACAGGCTTTGAAGCGGAAGCCAGATTTGTTCCTGTGTGAGCGACTGGATGTCAAAGC
  TGTGTTCCAGTGTGCCGTGCTGGCCCTCAAGTTCCCTGAGGCACCTACTGTCAAGGCCTCCTGTGGCTTC
  TTTACTGAGCTGCTGCCTCGATGTGGGGAAATAGAGTCTGTGGGGAAAGTGGTACAGGAAGATGGCCGTA
  TGCTGCTCATAGCAGTGCTGGAGGCCATCGGGGGCCAGGCCTCCCGTAGCCTCATGGACTGTTTTGCCGA
  CATCCTGTTCGCCCTGAACAAACACTGCTTCAGCCTCCTGAGCATGTGGATCAAGGAGGCTCTGCAGCCA
  CCTGGTTTTCCATCTGCCCGTCTCAGCCCTGAGCAGAAGGACACCTTTAGCCAGCAGATACTTCGTGAGC
  GAGTAAACAAGAGACGGGTGAAGGAGATGGTGAAGGAATTTACACTGCTCTGCCGGGGGCTACATGGCAC
  GGACTACACAGCTGACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211326 representing NM_146152
  Red=Cloning site Green=Tags(s)
  
  MERREEQLGAAGAGAAPALDFTVENVEKALHQLYYDPNIDNKNLAQKWLMQAQVSPQAWHFSWQLLQPDK
  VPEIQYFGASALHIKISRYWSDIPTDQYESLKAQLFTQITRFASGSKIVLTRLCVALASLALSMMPDAWP
  CAVADMVRLFQAEDSPVDSQGRCLALLELLTVLPEEFQTSRLPQYRKGLVRTSLAVECGTVFPLLEQLLQ
  QPSSPSCVRQKVLKCFSSWVQLEVPLQDCEALIQAAFAALQDSELFDSSVEAIVNAISQPDAQRYVNTLL
  KLIPLVLGLQEQLRQAVQNGDMETSHGICRIAVALGENHSRALLDQVEHWQSFLALVNMIMFCTGIPGHY
  PVNETTSSLTLTFWYTLQDDILSFEAEKQAVYQQVYRPVYFQLVDVLLHKAQFPSDEEYGFWSSDEKEQF
  RIYRVDISDTLMYVYEMLGAELLSNLYDKLGRLLTSSEEPYSWQHTEALLYGFQSIAETIDVNYSDVVPG
  LIGLIPRISISNVQLADTVMFTIGALSEWLADHPVMINSVLPLVLHALGNPELSVSSVSTLKKICRECKY
  DLPPYAANIVAVSQDVLMKQIHKTSQCMWLMQALGFLLSALQVEEILKNLHSLISPYIQQLEKLAEEIPN
  PSNKLAIVHILGLLSNLFTTLDVSHHEDDHEGPELRKLPVPQGPNPVVVVLQQVFQLIQKVLSKWLNDAQ
  VVEAVCAIFEKSVKTLLDDFAPMVPQLCEMLGRMYSTVPQASALDLTRQLVHIFAHEPAHFPPIEALFLL
  VTSVTLSLFQQGPRDHPDIVDSFMQLLAQALKRKPDLFLCERLDVKAVFQCAVLALKFPEAPTVKASCGF
  FTELLPRCGEIESVGKVVQEDGRMLLIAVLEAIGGQASRSLMDCFADILFALNKHCFSLLSMWIKEALQP
  PGFPSARLSPEQKDTFSQQILRERVNKRRVKEMVKEFTLLCRGLHGTDYTADY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146152
• ORF Size: 2889 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146152.3, NP_666264.1
• RefSeq Size: 3588 bp
• RefSeq ORF: 2892 bp
• Locus ID: 230673
• UniProt ID: Q8K0C1
• Cytogenetics: 4 D2.1
• MW: 108.2 kDa
• Gene Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of the paired homeobox family. Also mediates nuclear export of eIF-1A, and the cytoplasmic release of eIF-1A is triggered by the loading of import substrates onto IPO13 (By similarity).[UniProtKB/Swiss-Prot Function]