4933427D14Rik (NM_028963) Mouse Tagged ORF Clone

CAT#: MR211309

4933427D14Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933427D14 gene (4933427D14Rik)

Product Images

Circular map for MR211309

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 4933427D14Rik
• Synonyms: C85113; Kiaa0753; MNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211309 representing NM_028963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCAAGCCAGCCAGCTTCTACATGTGTTCATCTAGCACCCAGTCTCCAACTGGATGCCATGAGTG
  ACCCCAGGAACCTTCAGCCCCAGAACCAGCTGCAATTTAATAGGAATGTTCCTACAAATCCCAGCAACTT
  GGCAGTCCGGTACTCTTGCCCACATGGAATTAAAATTGAAAAACTGAAACACTCATACAATGAATTGAAT
  CATGGTAAAGACTTGGATTTTAAAGCTGGAAATGAATTAAGCAGTTCTGTCTCATTTTCTGTCATATCAG
  AAGAGAGGCTTAGCTATGCTGTCCACCTAGCCAAGAGAGATGTAAAACGAAGACAATTTGAAGAACACGT
  GAAAGATCATCTCAGAAGTCTGCCCCATAGCTCTCACAAAGGTATGCACACCAAGGTGGAAAGGAAGGAC
  TCCAAGAGTCAGGATGTCTGTCATTGCAGCCACCAGCCGTCCAGAGTAAGCCTCTCCAGCTCGGGCGCTA
  AGGTGTATGTGTACACATCTCAGCCAGGACGAGCTGATCTTACTGTGCCAAATTCACCACCTACCCATGA
  TCCAGGACTTCAGCCGCAACCCAGAATAGAGGAGAACAAAAACCTGTGGGAACAGAAAGGCCTGCTAGAA
  GTTCAGCGACTCCAAAAGGAATTGAGTGGTTGTATCCGTAAGATAGAAGCACTAACGAAAAAAGATAGAC
  TAGAAGAGGCTTTGGATCCAGATGAAGAGCATCGAATCCGAGTCAGGAGACAGGAGCAAGCCGTGCGCTG
  TGCCCGCATGCTCTACGTTCTTCAGCAGCAGGTAAAAGAAATCCAGGAAGAATTGGATAAATTGAGTCCA
  CAAAAAATTAAACATACTAAGAAGTCATGGGCAGTGTCTAGGCTGGCAGCCGCTCATCGAGCAGCCATCC
  GGGCCTTGCAAGTGTTTGTCACTCAGTTTACTGACCGAGGGGAGCACCCAGTTCCTGCTCGGTGTAGGGA
  GCTGGGCAGCCTTATTCGTCAGCTGTCGCTGTGTTCCGCCAAGCTGGATGCTGATCCTTCTGTGCCAGAC
  GTGGTCATAGACATCTTGCAGCAAATCGAGGCTTTGGAGTCTCTCCTGGAAAAGAAATTGTCACCTAAAA
  AGGCAAAGAAATGTTTCACAGAAATTCGGAGCAGATTTCCTGTTGGTAGCCAAAAGATCTTAGAGAGATG
  GCCAACTGTACTGCCCAAAAGTGAGAGGAGACCCTTGGTCACAAAGGAGACATTCCCACAGGAAACCAGT
  CGACCTCCTGTGGCAAAGAGGCTTCTTGCAGCTATGTGTCAGCCTGATGGGGAGCTTCAGAGGCTGGAGA
  GTGAACCCGATGTGCTGGATGCACATCTTCTTCCAGAAGAAGCCCCTCGTATAGAGGACAATGGTACAGA
  CTTCAAAGACGGAACATTAACCCCAGCCAAACCACAGGCAGTGAGAAGGAAGGCTTTGACAGGGTCCATG
  CCGATTAGGAAGAAAGATACTGTGGAGTCAGCCAGACCACAGCAGGGACTCCACATAGCTGAAAGAAATC
  GACCAAACCAACCTTACAGCAAAAGCAGGTTACAGCAGACAACAGTGTCATCCAGATTAAAAATGAACCG
  ACAGCCTATGAAAGACCATAGGGCTCCTTGGATACCTCCAAACCCCACATCCCCACCAGCCTCTCCTAAA
  TGTGCTGCGTGGATGAAGGTGAAGTACAGCCCCAGGGATGCTGCAAAGGAGCAGTCCCTACAGCAAGAGG
  ATACCCACAAGGAAAGTCAGCTGAGAGGTGATGCTGAGCAAGAAGCAGCCAGGCTTTCTTGGCCAGATGC
  TGAGTCTTCCAAAAGACTGAAGGAACTAGAAGAATTGGAAGCCAAAGAAATGGAAAGAAAGCAAAAGCAG
  AGGCTCAATTGGCTTGAGGCTGAGACTTCTAGAAGAACCAAAGAGTTGGATGAACTGAAAGCTGAAGAGA
  TGGATAGACTTCAAAAATTAAGTGTCTCTGCCACCCAGTTAGCTGACAAGGTGGAGGAGGCAGTTCTGGA
  GCGGTTGAAGCCCCTCTTGATCAAGGCCCAGAGAGTGAATTCTTCGGTGGAAGCAAATAGTCATTTGAAG
  GATCGTCCGTCACGGCACGCAGCAGCAGCAGCCCAGCCTGCCGAGCAGGCTTCAGATGTACGTTTTGAAT
  CCAGGAATATTCCTCAGCTGCGTGACTGTTTGGAAGATACTGCTCATGAGCTCTGGGCTAGGACTCAGGA
  TAAGATCTTGGGGTCAGAAACTTCAGCCAGATTGGGGGACAGCAAGGACAGCCCAGATCTAGAGACCATG
  ATGCTCCGGATGGAAGAAATGGAAAAATACCAGGAGACAGTTCGCCAAAGATATAATAAAATTGTATACG
  CTGACCCTCACTTATGGATGCACGAAGAAAGAAATGACCAGAACACCCCAGCTGTAAGTGAAGGACCTTT
  GGCTTCCCACCCAATCAAGATCACCAAGACAGCAACTCAAAAGTGCCCTGCTGTGAACATCTTGTTAGAA
  AGGCCCTGCAATGCCAACTCACTGGATGAAAGTGTAACAACAGAGGAGGGATCGGAGAAAAGGGAGGCTC
  CCCTGCCCCTCTCCAGAGAAGACCTGCATCAGAGAAAAGGGCAAACTCCCCTCTCAGTCCCACCCAGGAT
  GCGGCACAGCATTGGTGCTTACTGTAGCCGGTTTGAGCAATTCCTACGGATCATAGCTCATGAGGCTATA
  GGCTCCTTCAACCCATGGCTAATAGCTGAAAGCTTTTCAGATGAGCTGGTAGATGAAGCACTGGGGGCCG
  TGGCTGCAGAACTGCAGGATGTGTGTGAAGACTATGCAGAAGCTGTGTTCACCTCAGAGTTCCTAGAGGC
  CGCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211309 representing NM_028963
  Red=Cloning site Green=Tags(s)
  
  MGPSQPASTCVHLAPSLQLDAMSDPRNLQPQNQLQFNRNVPTNPSNLAVRYSCPHGIKIEKLKHSYNELN
  HGKDLDFKAGNELSSSVSFSVISEERLSYAVHLAKRDVKRRQFEEHVKDHLRSLPHSSHKGMHTKVERKD
  SKSQDVCHCSHQPSRVSLSSSGAKVYVYTSQPGRADLTVPNSPPTHDPGLQPQPRIEENKNLWEQKGLLE
  VQRLQKELSGCIRKIEALTKKDRLEEALDPDEEHRIRVRRQEQAVRCARMLYVLQQQVKEIQEELDKLSP
  QKIKHTKKSWAVSRLAAAHRAAIRALQVFVTQFTDRGEHPVPARCRELGSLIRQLSLCSAKLDADPSVPD
  VVIDILQQIEALESLLEKKLSPKKAKKCFTEIRSRFPVGSQKILERWPTVLPKSERRPLVTKETFPQETS
  RPPVAKRLLAAMCQPDGELQRLESEPDVLDAHLLPEEAPRIEDNGTDFKDGTLTPAKPQAVRRKALTGSM
  PIRKKDTVESARPQQGLHIAERNRPNQPYSKSRLQQTTVSSRLKMNRQPMKDHRAPWIPPNPTSPPASPK
  CAAWMKVKYSPRDAAKEQSLQQEDTHKESQLRGDAEQEAARLSWPDAESSKRLKELEELEAKEMERKQKQ
  RLNWLEAETSRRTKELDELKAEEMDRLQKLSVSATQLADKVEEAVLERLKPLLIKAQRVNSSVEANSHLK
  DRPSRHAAAAAQPAEQASDVRFESRNIPQLRDCLEDTAHELWARTQDKILGSETSARLGDSKDSPDLETM
  MLRMEEMEKYQETVRQRYNKIVYADPHLWMHEERNDQNTPAVSEGPLASHPIKITKTATQKCPAVNILLE
  RPCNANSLDESVTTEEGSEKREAPLPLSREDLHQRKGQTPLSVPPRMRHSIGAYCSRFEQFLRIIAHEAI
  GSFNPWLIAESFSDELVDEALGAVAAELQDVCEDYAEAVFTSEFLEAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028963
• ORF Size: 2877 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028963.1
• RefSeq Size: 4332 bp
• RefSeq ORF: 2880 bp
• Locus ID: 74477
• UniProt ID: Q6A000
• Cytogenetics: 11 B4
• MW: 109.2 kDa
• Gene Summary: Involved in centriole duplication. Positively regulates CEP63 centrosomal localization. Required for WDR62 centrosomal localization and promotes the centrosomal localization of CDK2.[UniProtKB/Swiss-Prot Function]