Mkl1 (NM_001082536) Mouse Tagged ORF Clone

CAT#: MR211216

Mkl1 (Myc-DDK-tagged) - Mouse MKL (megakaryoblastic leukemia)/myocardin-like 1 (Mkl1), transcript variant 2

Product Images

Circular map for MR211216

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mkl1
• Synonyms: AMKL; Bsac; Mal; Mrtf-A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211216 representing NM_001082536
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCCTTTGAAAAGCCCCGCTGCATTTCATGAGCAGAGAAGAAGCCTGGAGCGGGCCAGGACCGAGG
  ACTATTTGAAACGGAAGATCCGTTCCCGGCCCGAGAGAGCAGAGCTGGTCAGGATGCACATTCTGGAAGA
  GACCTCGGCTGAGCCTTCGCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGGCTGGCTGATGACCTC
  AATGAAAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAATATCCTGCCTGTGGAGTCCA
  GCCTGAAGGAGGCTATCATTGTGGGCCAGGTAAATTACCCAAAGGTAGCAGACAGTTCCTCCTTCGACGA
  GGACAGCAGCGATGCCCTGTCTCCTGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCAGTGCCATCACCC
  TTGGAGTCCCGAGTCAGTGATCCACTGCCCAGTGCCACCTCCATATCACCCACTCAGGTTCTTTCTCAAC
  TCCCAATGGCTCCGGATCCTGGAGAGACGCTTTTTCTGGCAGAGCAGCCTCCTCTGCCTCCCGCACCTCT
  GCTGCCCCCAAGCCTAGCCAATGGAAGCATCGTCCCCACTGCCAAGCCTGCTCCCACACTCATCAAGCAA
  AGCCAACCCAAGTCTGCCAGCGAGAAATCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAGCCAAAGGTGA
  AGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAAGGGGGCGCCCGCCATGGACTC
  CTCCTATGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTGCAGCTGCAGATCCTCAACCAGCAGCAGCAG
  CAGCAGCAGCAACAGCACTACAACTACCAGGCCATCCTGCCTGCCCCTCCCAAGCCCTCGGCTGAGACTC
  CTGGAAGCAGTGCCCCTACCCCATCACGCAGCCTCTCCACCAGTAGCAGCCCCAGCTCAGGCACCCCAGG
  GCCCAGCGGGCTGGCACGCCAGAGCAGCACCGCACTAGCTGCCAAACCAGGAGCCCTGCCAGCCAACCTG
  GATGACATGAAGGTGGCAGAGCTGAAGCAAGAACTGAAGTTGCGGTCCCTTCCCGTCTCAGGCACCAAGA
  CAGAGCTGATAGAACGCCTGCGTGCCTACCAAGACCAAGTCAGCCCAGCTCCAGGAGCCCCCAAGGCCCC
  TGCCACCACCTCTGTGCTGTCCAAGGCTGGTGAGGTAGTGGTCGCCTTCCCTGCGGCCCTGCTAAGCACA
  GGGTCAGCTCTTGTAACAGCAGGCCTTGCACCAGCTGAGATGGTGGTGGCCACAGTAACCAGCAATGGCA
  TGGTGAAGTTTGGCAGCACAGGCTCCACACCCCCCGTGTCTCCCACCCCTTCAGAGCGCTCACTGCTCAG
  CACGGGTGATGAGAATTCCACACCTGGGGATGCCTTTGGTGAAATGGTGACATCGCCGCTGACACAGCTC
  ACCCTGCAGGCCTCCCCACTGCAGATCGTGAAGGAGGAGGGTGCCCGTGCTGCGTCCTGCTGTCTAAGCC
  CTGGTGCTCGGGCTGAGCTGGAGGGACTGGACAAGGACCAGATGCTGCAGGAGAAGGACAAGCAGATTGA
  GGAGCTGACCCGAATGCTCCAACAGAAGCAGCAGCTGGTTGAGCTGCTGCGGCTACAGCTGGAGCAGCAG
  AAGCGGGCCCAGCAGCCAGCCCCAGCCAGCAGCCCTGTGAAGAGGGAAAGTGGTTTCTCCAGTTGCCAGC
  TGAGCTGCCAGCCCCAGGGCTCTGCCCATGCTTTTGGCTCTGGCCTAGTGGTTCCCACTACCAACCATGG
  AGACACTCAGGCCCCAGCGCCAGAGTCCCCACCTGTGGTGGTGAAGCAGGAAGCTGGGCCACCTGAGCCA
  GATCTGGCCCCCAGCTCCCAGCTGCTCTTGGGCTCCCAGGGCACCAGCTTCCTCAAGAGGGTCAGCCCTC
  CTACCCTGGTCACTGACTCTACAGGGACTCACCTCATCCTCACTGTGACCAATAAGAGTGCTGATGGCCC
  TGGCTTGCCTGCAGGGAGCCCCCAGCAGCCCTTGTCCCAGCCTGGTTCTCCAGCCCCTGGTCCACCTGCC
  CAGATGGACCTGGAGCACCCACCTCAGCCTCCGTTTGCAACCCCCACATCTCTGCTGAAGAAGGAGCCCC
  CTGGTTATGAAGAGACTGTGACCCAGCAGCCTAAGCAGCAGGAAAATGGCTCCTCCAGTCAGCACATGGA
  TGATCTGTTTGATATTCTTATTCAGAGTGGAGAGATTTCAGCAGATTTCAAAGAGCCACCATCCCTACCA
  GGCAAGGAAAAGTCACCTCCAGCAGCAGCAGCGTATGGGCCTCCATTGACACCACAACCCTCGCCTTTGA
  GTGAACTCCCCCAAGCTGCTCCTCCACCAGGTTCCCCCACCCTCCCAGGGCGCCTTGAAGACTTCCTGGA
  GAGCAGCACAGGGCTGCCCCTGCTGACAAGTGGGCACGAGGGACCAGAACCCCTTTCCCTCATTGATGAC
  CTCCACAGCCAGATGCTGAGCAGCTCCGCCATCCTGGACCACCCCCCATCACCCATGGACACCTCTGAAT
  TGCACTTTGCTCCTGAGCCCAGCAGTGGTATGGGCCTGGACCTGGCTGTTGGCCACCTGGACAGCATGGA
  CTGGCTGGAGCTGTCGTCTGGTGGCCCTGTGCTCAGCCTGGCTCCCCTCAGCACTGCAGCCCCCAGCCTC
  TTCTCGATGGACTTCCTGGATGGACACGACTTGCAGCTCCACTGGGATTCCTGCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211216 representing NM_001082536
  Red=Cloning site Green=Tags(s)
  
  MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERAELVRMHILEETSAEPSLQAKQLKLKRARLADDL
  NEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSP
  LESRVSDPLPSATSISPTQVLSQLPMAPDPGETLFLAEQPPLPPAPLLPPSLANGSIVPTAKPAPTLIKQ
  SQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDKGAPAMDSSYAKILQQQQLFLQLQILNQQQQ
  QQQQQHYNYQAILPAPPKPSAETPGSSAPTPSRSLSTSSSPSSGTPGPSGLARQSSTALAAKPGALPANL
  DDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQVSPAPGAPKAPATTSVLSKAGEVVVAFPAALLST
  GSALVTAGLAPAEMVVATVTSNGMVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDAFGEMVTSPLTQL
  TLQASPLQIVKEEGARAASCCLSPGARAELEGLDKDQMLQEKDKQIEELTRMLQQKQQLVELLRLQLEQQ
  KRAQQPAPASSPVKRESGFSSCQLSCQPQGSAHAFGSGLVVPTTNHGDTQAPAPESPPVVVKQEAGPPEP
  DLAPSSQLLLGSQGTSFLKRVSPPTLVTDSTGTHLILTVTNKSADGPGLPAGSPQQPLSQPGSPAPGPPA
  QMDLEHPPQPPFATPTSLLKKEPPGYEETVTQQPKQQENGSSSQHMDDLFDILIQSGEISADFKEPPSLP
  GKEKSPPAAAAYGPPLTPQPSPLSELPQAAPPPGSPTLPGRLEDFLESSTGLPLLTSGHEGPEPLSLIDD
  LHSQMLSSSAILDHPPSPMDTSELHFAPEPSSGMGLDLAVGHLDSMDWLELSSGGPVLSLAPLSTAAPSL
  FSMDFLDGHDLQLHWDSCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001082536
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001082536.1, NP_001076005.1
• RefSeq Size: 4366 bp
• RefSeq ORF: 2790 bp
• Locus ID: 223701
• UniProt ID: Q8K4J6
• Cytogenetics: 15 E1
• MW: 98.9 kDa
• Gene Summary: Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12019265, PubMed:12732141, PubMed:17588931, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex (PubMed:12732141, PubMed:17588931). Activity is also regulated by filamentous actin (F-actin) in the nucleus (PubMed:23558171, PubMed:25759381).[UniProtKB/Swiss-Prot Function]