Slc8a2 (NM_148946) Mouse Tagged ORF Clone

CAT#: MR211188

Slc8a2 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 2 (Slc8a2)

Product Images

Circular map for MR211188

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc8a2
• Synonyms: Ncx2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211188 representing NM_148946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCTTGGCTTTGATGGGGGTTGTTCTCCTTCTGGGGGTACCCCACTGCTTGGGGGAAGCCACTC
  CGACCCCTTCCCTACCGCCTCCCACAGCTAATGACAGCGATGCCAGCCCAGAAGGCTGCCAAGGTTCCTA
  CCGCTGCCAACCAGGTGTGCTGCTGCCTGTGTGGGAACCCGAGGACCCATCGCTGGGCGACAAGGTTGCA
  CGGGCCGTGGTGTACTTTGTGGCCATGGTCTACATGTTCCTGGGTGTGTCTATCATTGCCGATCGATTTA
  TGGCATCCATTGAGGTCATCACATCCAAGGAGAAAGAGATCACCATCACCAAGGCAAATGGGGAGACCAG
  CGTGGGCACGGTGCGCATCTGGAACGAGACGGTGTCCAACCTTACACTCATGGCCCTGGGCTCCTCAGCG
  CCTGAGATTCTGTTGACTGTCATCGAGGTCTGTGGCCACAACTTCCAGGCCGGTGAGCTAGGCCCAGGCA
  CCATCGTGGGCAGTGCCGCCTTCAACATGTTTGTGGTCATTGCTGTTTGTGTGTATGTCATCCCGGCTGG
  CGAGAGCCGTAAGATCAAGCACCTGAGGGTCTTCTTTGTCACAGCCTCCTGGAGCATCTTTGCCTATGTC
  TGGCTTTATCTCATTCTAGCAGTTTTCTCCCCAGGTGTAGTCCAGGTGTGGGAGGCACTGCTCACACTGA
  TCTTCTTCCCGGTGTGTGTGGTGTTTGCCTGGATGGCGGACAAGCGACTGCTCTTCTACAAGTACGTGTA
  CAAGCGCTACCGCACCGACCCTCGCAGTGGAATCATCATCGGGGCAGAGGGAGACCCACCCAAGAGCATC
  GAGCTGGACGGCACATTCGTGGGCACTGAGGTCCCTGGCGAGCTGGGCGCATTGGGCACAGGTCCTGCTG
  AGGCGCGTGAACTAGATGCCAGCCGGCGTGAGGTCATCCAGATCCTTAAGGACTTGAAGCAGAAGCACCC
  GGATAAGGACCTGGAGCAGCTGATGGGCATCGCCAAGTACTATGCACTGCTGCACCAGCAGAAGAGCCGC
  GCCTTCTACCGCATCCAGGCCACGCGGCTGATGACAGGTGCGGGCAATGTGCTGCGCAGACATGCTGCGG
  ATGCTGCCCGCAGGCCGGGAGCCACCGATGGTGCCCCCGATGATGAGGACGATGGTGCCAGTCGCATCTT
  CTTTGAGCCCAGCCTCTATCACTGCCTGGAAAACTGCGGGTCAGTGCTGCTGTCCGTGGCTTGCCAGGGC
  GGTGAGGGCAACAGCACCTTCTACGTGGACTACCGTACCGAGGACGGTTCTGCAAAGGCAGGCTCCGATT
  ATGAGTACAGCGAGGGCACGCTGGTGTTCAAGCCCGGGGAGACGCAGAAGGACCTGCGCATCGGGATCAT
  CGACGACGACATCTTCGAGGAGGATGAGCACTTCTTCGTGAGGCTGCTGAACCTGCGTGTGGGCGATGCT
  CAGGGCATGTTCGAGCCCGACGGCGGTGGGCGGCCCAAGGGGCGGCTGGTGGCGCCGCTGCTGGCCACTG
  TCACCATCCTGGACGACGACCACGCGGGCATCTTCTCCTTCCAGGACCGCCTGCTGCATGTGAGCGAGTG
  CATGGGCACTGTGGATGTGCGCGTGGTTCGCAGCTCGGGCGCCCGTGGCACTGTACGCCTCCCCTACCGC
  ACAGTGGACGGCACGGCCCGTGGCGGTGGTGTACATTACGAGGATGCTTGTGGAGAGCTGGAGTTCGGCG
  ATGATGAGACCATGAAAACTCTTCAGGTCAAGATAGTGGATGATGAAGAGTATGAGAAGAAGGACAACTT
  CTTCATTGAGCTGGGCCAGCCCCAGTGGCTTAAGCGAGGCATCTCAGCTCTGCTACTCAACCAAGGGAAT
  GGAGACAAGAAGATAACTGCAGAGCAGGAGGAGGCCCAGAGGATAGCAGAGATGGGCAAGCCAGTTCTTG
  GGGAGAACAATCGCCTCGAGGTCATCATCGAGGAGTCTTATGACTTTAAGAACACGGTGGATAAACTCAT
  AAAGAAAACGAACCTGGCCTTGGTGATTGGGACTCACTCATGGAGGGAGCAGTTTATAGAGGCAGTTACC
  GTAAGCGCAGGGGACGAGGAGGAGGATGAGGATGGACCTCGTGAGGAGCGGCTGCCGTCCTGCTTTGACT
  ACGTGATGCACTTCCTGACGGTATTCTGGAAAGTTCTATTCGCCTGCGTTCCACCCACGGAGTACTGCAA
  TGGCTGGGCCTGCTTTGGTGTCTGCATCCTGGTCATTGGTGTGCTCACTGCCCTCATCGGAGACCTGGCC
  TCACACTTTGGGTGCACCGTGGGCCTCAAGGACTCAGTCAACGCCGTGGTCTTCGTAGCCCTGGGCACCT
  CCATCCCTGACACGTTCGCCAGCAAGGTGGCCGCGCTGCAGGACCAGTGTGCCGACGCGTCCATCGGTAA
  CGTGACCGGCTCCAATGCGGTGAACGTGTTCCTGGGCCTGGGTGTGGCCTGGTCGGTGGCAGCGGTATAC
  TGGGCGGTGCAGGGTCGCCCCTTCGAGGTGCGTGCAGGCACGCTGGCCTTCTCGGTCACGCTGTTCACCG
  TCTTCGCCTTCGTGTGCATCGCAGTGCTGTTGTACCGGCGCCGGCCGCAGATTGGCGGCGAGCTGGGCGG
  CCCGCGGGGACCCAAGCTGGCCACCACCGCTCTCTTCCTGGGCCTCTGGTTCCTCTACATTCTCTTCTCC
  AGCCTGGAGGCTTACTGCCACATCCGGGGCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211188 representing NM_148946
  Red=Cloning site Green=Tags(s)
  
  MAPLALMGVVLLLGVPHCLGEATPTPSLPPPTANDSDASPEGCQGSYRCQPGVLLPVWEPEDPSLGDKVA
  RAVVYFVAMVYMFLGVSIIADRFMASIEVITSKEKEITITKANGETSVGTVRIWNETVSNLTLMALGSSA
  PEILLTVIEVCGHNFQAGELGPGTIVGSAAFNMFVVIAVCVYVIPAGESRKIKHLRVFFVTASWSIFAYV
  WLYLILAVFSPGVVQVWEALLTLIFFPVCVVFAWMADKRLLFYKYVYKRYRTDPRSGIIIGAEGDPPKSI
  ELDGTFVGTEVPGELGALGTGPAEARELDASRREVIQILKDLKQKHPDKDLEQLMGIAKYYALLHQQKSR
  AFYRIQATRLMTGAGNVLRRHAADAARRPGATDGAPDDEDDGASRIFFEPSLYHCLENCGSVLLSVACQG
  GEGNSTFYVDYRTEDGSAKAGSDYEYSEGTLVFKPGETQKDLRIGIIDDDIFEEDEHFFVRLLNLRVGDA
  QGMFEPDGGGRPKGRLVAPLLATVTILDDDHAGIFSFQDRLLHVSECMGTVDVRVVRSSGARGTVRLPYR
  TVDGTARGGGVHYEDACGELEFGDDETMKTLQVKIVDDEEYEKKDNFFIELGQPQWLKRGISALLLNQGN
  GDKKITAEQEEAQRIAEMGKPVLGENNRLEVIIEESYDFKNTVDKLIKKTNLALVIGTHSWREQFIEAVT
  VSAGDEEEDEDGPREERLPSCFDYVMHFLTVFWKVLFACVPPTEYCNGWACFGVCILVIGVLTALIGDLA
  SHFGCTVGLKDSVNAVVFVALGTSIPDTFASKVAALQDQCADASIGNVTGSNAVNVFLGLGVAWSVAAVY
  WAVQGRPFEVRAGTLAFSVTLFTVFAFVCIAVLLYRRRPQIGGELGGPRGPKLATTALFLGLWFLYILFS
  SLEAYCHIRGF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148946
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148946.3
• RefSeq Size: 4252 bp
• RefSeq ORF: 2766 bp
• Locus ID: 110891
• UniProt ID: Q8K596
• Cytogenetics: 7 8.75 cM
• MW: 101.2 kDa
• Gene Summary: Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells. Contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:12818181). Plays a role in regulating urinary Ca(2+) and Na(+) excretion (PubMed:25498502).[UniProtKB/Swiss-Prot Function]