Extl3 (NM_018788) Mouse Tagged ORF Clone

CAT#: MR211177

Extl3 (Myc-DDK-tagged) - Mouse exostoses (multiple)-like 3 (Extl3)

Product Images

Circular map for MR211177

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Extl3
• Synonyms: 2900009G18Rik; AU040105; Ext1l; mKIAA0519
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211177 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGCTATACCATGTTGCGGAATGGGGGAGTGGGGAACGGTGGTCAGACCTGTATGCTGCGCTGGT
  CCAATCGCATCCGGCTGACATGGCTGAGTTTCACGCTGTTCATCATCCTCGTCTTCTTCCCCCTCATTGC
  TCACTATTACCTCACCACTCTGGACGAGGCAGACGAGGCTGGCAAGCGCATCTTCGGCCCTCGGGCTGGC
  AGTGAGCTCTGTGAGGTAAAGCACGTCCTCGATCTCTGTCGGATTCGTGAGTCTGTGAGCGAAGAGCTTC
  TACAGCTCGAAGCCAAGCGGCAGGAGCTGAACAGCGAGATTGCCAAGCTGAACCTCAAGATTGAAGCCTG
  TAAGAAGAGCATAGAGAATGCCAAGCAGGACCTGCTGCAGCTCAAGAATGTCATTAGCCAGACAGAGCAC
  TCCTACAAGGAGCTGATGGCCCAGAACCAGCCCAAACTGTCCCTGCCCATCCGACTGCTCCCCGAGAAGG
  ACGATGCCGGCCTTCCACCCCCCAAGGTCACTCGGGGTTGCCGCCTTCACAACTGCTTTGATTACTCTCG
  TTGTCCTCTGACGTCTGGCTTTCCCGTCTACGTCTATGACAGTGACCAGTTTGCCTTTGGGAGCTACCTG
  GACCCTTTGGTCAAGCAGGCTTTTCAGGCTACAGTGAGAGCCAACGTTTATGTTACAGAAAATGCGGCCA
  TCGCCTGCCTGTATGTGGTGTTAGTGGGAGAAATGCAAGAGCCCACTGTGCTGCGGCCTGCCGACCTTGA
  AAAGCAGCTGTTTTCTCTGCCACACTGGAGGACAGATGGGCACAACCACGTCATTATCAACCTGTCCCGG
  AAGTCAGACACACAGAATCTACTGTACAACGTCAGTACAGGCCGGGCCATGGTGGCCCAGTCCACCTTCT
  ATGCTGCCCAGTACAGAGCTGGCTTTGACCTGGTCGTGTCACCCCTTGTCCATGCTATGTCTGAACCCAA
  CTTCATGGAAATCCCACCGCAGGTGCCAGTTAAGCGGAAATATCTCTTCACTTTCCAGGGCGAGAAGATC
  GAGTCTCTGAGATCTAGCCTTCAGGAGGCCCGTTCCTTCGAGGAAGAGATGGAGGGCGACCCTCCGGCCG
  ACTATGACGATCGCATCATTGCCACCCTAAAGGCCGTACAGGACAGCAAGCTGGATCAGGTGCTGGTAGA
  ATTCACTTGCAAAAACCAGCCGAAGCCCAGCCTGCCGACTGAGTGGGCACTGTGTGGGGAGCGGGAAGAC
  CGCCTGGAGTTACTGAAGCTCTCCACCTTCGCCCTCATCATCACTCCCGGGGACCCGCACCTGCTCATTT
  CATCTGGGTGTGCCACGCGGCTCTTCGAGGCCCTGGAGGTGGGGGCCGTGCCGGTGGTGCTCGGGGAGCA
  GGTGCAGCTCCCGTACCACGACATGCTGCAGTGGAACGAGGCCGCCCTGGTGGTGCCCAAGCCTCGCGTC
  ACAGAGGTCCACTTCCTGTTACGAAGTCTTTCAGACAGTGATCTGTTGGCCATGAGGCGGCAAGGCCGCT
  TTCTCTGGGAGACCTACTTCTCCACCGCAGACAGTATTTTTAATACCGTGCTGGCCATGATTAGGACTCG
  AATTCAGATCCCAGCTGCTCCCATCCGGGAAGAGGTAGCGGCTGAGATCCCCCATCGTTCAGGCAAAGCA
  GCTGGAACTGACCCCAACATGGCTGACAATGGGGACCTGGACCTGGGGCCGGTAGAGACAGAACCACCCT
  ATGCCTCACCTAAATACCTCCGCAATTTCACTCTGACTGTCACAGACTGTTACCGTGGCTGGAACTCTGC
  CCCGGGACCCTTCCATCTTTTTCCCCACACACCCTTTGATCCTGTGTTGCCCTCTGAGGCCAAATTCCTG
  GGCTCAGGGACTGGATTTCGGCCCATCGGTGGCGGGGCTGGGGGCTCTGGCAAGGAGTTCCAGGCAGCGC
  TCGGAGGCAATGTCCAGCGGGAGCAGTTCACAGTTGTGATGCTGACCTACGAGCGGGAGGAAGTGCTCAT
  GAACTCCCTGGAGAGACTCAACGGCCTCCCCTACCTGAACAAGGTAGTGGTGGTGTGGAACTCTCCCAAG
  CTGCCCTCGGAGGACCTTTTGTGGCCAGACATTGGTGTCCCCATCATGGTCGTCCGTACTGAGAAGAACA
  GTTTGAACAATCGGTTCTTGCCCTGGAATGAGATTGAGACAGAGGCCATACTGTCCATCGACGATGATGC
  TCACCTCCGCCATGATGAAATCATGTTTGGGTTTCGGGTGTGGAGAGAAGCACGTGATCGCATTGTGGGT
  TTCCCTGGCCGGTACCATGCGTGGGACATACCACACCAGTCCTGGCTCTACAATTCCAACTACTCCTGTG
  AGCTGTCCATGGTGCTGACGGGCGCTGCCTTCTTTCACAAGTATTATGCCTACCTGTATTCTTATGTGAT
  GCCCCAGGCCATCCGGGACATGGTGGACGAGTACATCAACTGTGAGGATATCGCCATGAACTTCCTTGTC
  TCCCACATCACACGGAAACCCCCCATCAAGGTGACATCAAGGTGGACTTTTCGATGCCCAGGGTGCCCTC
  AGGCCCTGTCCCATGATGACTCTCATTTTCACGAGCGGCACAAGTGTATCAACTTTTTTGTCAAGGTGTA
  CGGCTATATGCCTCTCTTGTACACACAGTTCAGGGTGGACTCCGTGCTCTTCAAGACCCGCCTGCCCCAT
  GACAAGACCAAGTGCTTCAAGTTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211177 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTGYTMLRNGGVGNGGQTCMLRWSNRIRLTWLSFTLFIILVFFPLIAHYYLTTLDEADEAGKRIFGPRAG
  SELCEVKHVLDLCRIRESVSEELLQLEAKRQELNSEIAKLNLKIEACKKSIENAKQDLLQLKNVISQTEH
  SYKELMAQNQPKLSLPIRLLPEKDDAGLPPPKVTRGCRLHNCFDYSRCPLTSGFPVYVYDSDQFAFGSYL
  DPLVKQAFQATVRANVYVTENAAIACLYVVLVGEMQEPTVLRPADLEKQLFSLPHWRTDGHNHVIINLSR
  KSDTQNLLYNVSTGRAMVAQSTFYAAQYRAGFDLVVSPLVHAMSEPNFMEIPPQVPVKRKYLFTFQGEKI
  ESLRSSLQEARSFEEEMEGDPPADYDDRIIATLKAVQDSKLDQVLVEFTCKNQPKPSLPTEWALCGERED
  RLELLKLSTFALIITPGDPHLLISSGCATRLFEALEVGAVPVVLGEQVQLPYHDMLQWNEAALVVPKPRV
  TEVHFLLRSLSDSDLLAMRRQGRFLWETYFSTADSIFNTVLAMIRTRIQIPAAPIREEVAAEIPHRSGKA
  AGTDPNMADNGDLDLGPVETEPPYASPKYLRNFTLTVTDCYRGWNSAPGPFHLFPHTPFDPVLPSEAKFL
  GSGTGFRPIGGGAGGSGKEFQAALGGNVQREQFTVVMLTYEREEVLMNSLERLNGLPYLNKVVVVWNSPK
  LPSEDLLWPDIGVPIMVVRTEKNSLNNRFLPWNEIETEAILSIDDDAHLRHDEIMFGFRVWREARDRIVG
  FPGRYHAWDIPHQSWLYNSNYSCELSMVLTGAAFFHKYYAYLYSYVMPQAIRDMVDEYINCEDIAMNFLV
  SHITRKPPIKVTSRWTFRCPGCPQALSHDDSHFHERHKCINFFVKVYGYMPLLYTQFRVDSVLFKTRLPH
  DKTKCFKFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018788
• ORF Size: 2760 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018788.3, NP_061258.2
• RefSeq Size: 5995 bp
• RefSeq ORF: 2760 bp
• Locus ID: 54616
• Cytogenetics: 14 D1
• MW: 104.5 kDa