Ano6 (NM_175344) Mouse Tagged ORF Clone

USD 830.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Ano6 (NM_175344) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ano6
Synonyms 2900059G15Rik; AA407480; AW554778; F730003B03Rik; Tmem16f
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR211153 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)



ATGCAGATGATGACTAGGAAGGTCCTGCTGAACATGGAGCTGGAGGAGGACGACGATGAGGATGGAGACA
TTGTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTT
CAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCGA
ATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAAC
AGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGATC
TGTTTCTGATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTATGCTGAG
ATCATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAACT
GGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTTT
TGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGC
CGCATTGTTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAATTTGGGATTA
ATAGACTGGTCAGCTCTGGAATCTACAAAGCAGCGTTTCCTCTGCACGACTGCAGATTCAACTATGAGTC
GGAGGACATCAGTTGTCCTAGCGAGCGTTACCTCCTGTACAGAGAATGGGCTCACCCTCGGAGTATATAC
AAGAAGCAGCCCTTGGATCTTATCAGGAAGTATTACGGCGAGAAGATTGGAATCTACTTTGCTTGGCTGG
GCTATTACACGCAGATGCTCCTTCTCGCAGCTGTGGTGGGCGTGGCCTGCTTCCTCTATGGATATCTTGA
TCAGGATAACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAGATCCTGATGTGCCCC
CAGTGTGACAGGCTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCTTCTAAGAAATTGTGCATCT
TTGACAGTTTTGGAACCCTGATCTTCGCTGTATTTATGGGAGTGTGGGTTACATTGTTTTTGGAGTTTTG
GAAGCGGCGCCAGGCAGAGCTCGAATATGAATGGGACACCGTTGAGCTACAGCAAGAGGAACAGGCCCGG
CCAGAGTATGAGGCCCAGTGCAATCACGTGGTGATAAACGAGATCACTCAGGAGGAGGAGCGCATCCCAT
TTACCACCTGTGGGAAGTGCATCCGGGTCACGCTGTGTGCGAGCGCCGTCTTCTTCTGGATCCTGCTCAT
CATCGCCTCTGTGATCGGGATCATTGTCTACAGGCTGTCCGTGTTCATCGTATTTTCCACAACGCTCCCC
AAGAACCCCAACGGGACAGACCCGATCCAGAAGTACCTGACCCCACAGATGGCCACATCCATCACAGCCT
CCATCATCAGCTTCATCATCATCATGATCCTCAACACGATCTACGAGAAGGTGGCCATCATGATCACCAA
CTTCGAGCTCCCAAGGACCCAGACGGATTATGAGAACAGCCTGACCATGAAGATGTTCTTGTTCCAGTTT
GTCAACTATTACTCCTCATGCTTCTACATCGCATTCTTCAAGGGCAAATTCGTGGGCTATCCCGGGGACC
CAGTGTACTTGCTGGGCAAATACAGAAGCGAAGAGTGTGACCCGGGGGGCTGCCTCCTTGAACTGACCAC
ACAGCTGACGATCATCATGGGGGGAAAGGCAATCTGGAACAACATACAAGAAGTCTTGCTCCCATGGGTT
ATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATT
ACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGTT
CGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAATA
AGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCG
CGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACGCCATGATCATTGCTTTCAC
ATCAGACATGATCCCCCGCCTGGTGTATTATTGGTCTTTCTCTATCCCTCCCTACGGGGACCACACTTAT
TACACCATGGACGGCTACATCAACAACACTCTCTCTGTCTTCAACATCACGGACTTCAAGAACACAGACA
AAGAAAACCCATACATTGGGCTTGGTAACTATACCTTGTGCAGGTACCGTGACTTCCGAAACCCACCTGG
GCACCCACAAGAGTATAAACACAACATCTACTATTGGCACGTGATTGCCGCCAAGCTGGCCTTCATCATT
GTCATGGAGCACATCATCTACTCTGTGAAGTTCTTCATTTCCTACGCAATTCCAGATGTGTCCAAAATCA
CGAAAAGCAAGATCAAGAGGGAGAAATACCTCACGCAGAAGTTGCTTCACGAGAGTCACCTCAAAGACCT
GACCAAAAACATGGGGATCATCGCCGAGAGGATAGGAGGAACGGTTGACAACAGCGTGCGGCCAAAACTC
GAA


>MR211153 protein sequence
Red=Cloning site Green=Tags(s)

MQMMTRKVLLNMELEEDDDEDGDIVLENFDQTIVCPTFGSLENQQDFRTPEFEEFNGKPDSLFFTDGQRR
IDFILVYEDESKKENNKKGTNEKQKRKRQAYESNLICHGLQLEATRSVSDDKLVFVKVHAPWEVLCTYAE
IMHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEKSRMNDFYILDRDSFFNPATRS
RIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCRFNYESEDISCPSERYLLYREWAHPRSIY
KKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLDQDNCTWSKEVCDPDIGGQILMCP
QCDRLCPFWRLNITCESSKKLCIFDSFGTLIFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQAR
PEYEAQCNHVVINEITQEEERIPFTTCGKCIRVTLCASAVFFWILLIIASVIGIIVYRLSVFIVFSTTLP
KNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQF
VNYYSSCFYIAFFKGKFVGYPGDPVYLLGKYRSEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWV
MNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEI
RVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSIPPYGDHTY
YTMDGYINNTLSVFNITDFKNTDKENPYIGLGNYTLCRYRDFRNPPGHPQEYKHNIYYWHVIAAKLAFII
VMEHIIYSVKFFISYAIPDVSKITKSKIKREKYLTQKLLHESHLKDLTKNMGIIAERIGGTVDNSVRPKL
E

Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_175344
ORF Size 2736 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175344.3, NM_175344.4, NP_780553.2
RefSeq Size 5662 bp
RefSeq ORF 2736 bp
Locus ID 105722
Cytogenetics 15 E3-F1
MW 106.3 kDa

Citations (1)

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