Ano6 (NM_175344) Mouse Tagged ORF Clone

CAT#: MR211153

Ano6 (Myc-DDK-tagged) - Mouse anoctamin 6 (Ano6)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ano6
• Synonyms: 2900059G15Rik; AA407480; AW554778; F730003B03Rik; Tmem16f
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211153 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  
  
  ATGCAGATGATGACTAGGAAGGTCCTGCTGAACATGGAGCTGGAGGAGGACGACGATGAGGATGGAGACA
  TTGTGCTGGAAAACTTTGACCAGACAATTGTCTGCCCCACCTTTGGATCACTGGAGAATCAGCAGGACTT
  CAGGACTCCAGAGTTTGAAGAATTTAACGGGAAGCCCGACTCCCTCTTTTTCACCGATGGCCAGAGGCGA
  ATCGACTTCATCCTCGTGTATGAAGATGAGAGCAAAAAGGAGAACAATAAGAAAGGGACAAATGAGAAAC
  AGAAGAGGAAAAGACAAGCATACGAATCTAACCTTATCTGCCATGGGCTGCAGCTGGAAGCAACAAGATC
  TGTTTCTGATGACAAGCTTGTGTTCGTAAAAGTGCACGCGCCCTGGGAAGTGCTGTGCACCTATGCTGAG
  ATCATGCACATCAAACTCCCGCTAAAGCCAAACGACCTGAAAACGCGCTCGCCCTTTGGCAACCTCAACT
  GGTTCACCAAGGTCCTCCGGGTGAACGAGAGTGTCATCAAGCCAGAGCAGGAGTTCTTCACTGCCCCTTT
  TGAGAAGAGCCGGATGAATGATTTCTACATCCTCGATAGAGATTCCTTCTTCAACCCTGCCACCAGAAGC
  CGCATTGTTTATTTCATCCTCTCTCGGGTCAAATACCAAGTGATGAACAACGTTAACAAATTTGGGATTA
  ATAGACTGGTCAGCTCTGGAATCTACAAAGCAGCGTTTCCTCTGCACGACTGCAGATTCAACTATGAGTC
  GGAGGACATCAGTTGTCCTAGCGAGCGTTACCTCCTGTACAGAGAATGGGCTCACCCTCGGAGTATATAC
  AAGAAGCAGCCCTTGGATCTTATCAGGAAGTATTACGGCGAGAAGATTGGAATCTACTTTGCTTGGCTGG
  GCTATTACACGCAGATGCTCCTTCTCGCAGCTGTGGTGGGCGTGGCCTGCTTCCTCTATGGATATCTTGA
  TCAGGATAACTGCACTTGGAGCAAAGAGGTCTGTGATCCTGACATCGGTGGCCAGATCCTGATGTGCCCC
  CAGTGTGACAGGCTGTGTCCGTTCTGGAGGCTGAATATCACCTGTGAATCTTCTAAGAAATTGTGCATCT
  TTGACAGTTTTGGAACCCTGATCTTCGCTGTATTTATGGGAGTGTGGGTTACATTGTTTTTGGAGTTTTG
  GAAGCGGCGCCAGGCAGAGCTCGAATATGAATGGGACACCGTTGAGCTACAGCAAGAGGAACAGGCCCGG
  CCAGAGTATGAGGCCCAGTGCAATCACGTGGTGATAAACGAGATCACTCAGGAGGAGGAGCGCATCCCAT
  TTACCACCTGTGGGAAGTGCATCCGGGTCACGCTGTGTGCGAGCGCCGTCTTCTTCTGGATCCTGCTCAT
  CATCGCCTCTGTGATCGGGATCATTGTCTACAGGCTGTCCGTGTTCATCGTATTTTCCACAACGCTCCCC
  AAGAACCCCAACGGGACAGACCCGATCCAGAAGTACCTGACCCCACAGATGGCCACATCCATCACAGCCT
  CCATCATCAGCTTCATCATCATCATGATCCTCAACACGATCTACGAGAAGGTGGCCATCATGATCACCAA
  CTTCGAGCTCCCAAGGACCCAGACGGATTATGAGAACAGCCTGACCATGAAGATGTTCTTGTTCCAGTTT
  GTCAACTATTACTCCTCATGCTTCTACATCGCATTCTTCAAGGGCAAATTCGTGGGCTATCCCGGGGACC
  CAGTGTACTTGCTGGGCAAATACAGAAGCGAAGAGTGTGACCCGGGGGGCTGCCTCCTTGAACTGACCAC
  ACAGCTGACGATCATCATGGGGGGAAAGGCAATCTGGAACAACATACAAGAAGTCTTGCTCCCATGGGTT
  ATGAATCTAATTGGACGATATAAAAGAGTCTCGGGATCAGAAAAGATAACCCCACGATGGGAACAGGATT
  ACCATCTGCAGCCCATGGGCAAGCTGGGATTGTTCTATGAGTATCTTGAAATGATTATTCAGTTTGGGTT
  CGTCACTTTATTTGTGGCCTCTTTCCCACTGGCTCCCCTGCTGGCTCTGGTGAACAATATTTTGGAAATA
  AGAGTGGACGCGTGGAAGCTCACAACCCAGTTTAGGCGCATGGTGCCCGAAAAAGCCCAGGATATCGGCG
  CGTGGCAGCCCATCATGCAAGGAATAGCCATCCTGGCCGTGGTGACCAACGCCATGATCATTGCTTTCAC
  ATCAGACATGATCCCCCGCCTGGTGTATTATTGGTCTTTCTCTATCCCTCCCTACGGGGACCACACTTAT
  TACACCATGGACGGCTACATCAACAACACTCTCTCTGTCTTCAACATCACGGACTTCAAGAACACAGACA
  AAGAAAACCCATACATTGGGCTTGGTAACTATACCTTGTGCAGGTACCGTGACTTCCGAAACCCACCTGG
  GCACCCACAAGAGTATAAACACAACATCTACTATTGGCACGTGATTGCCGCCAAGCTGGCCTTCATCATT
  GTCATGGAGCACATCATCTACTCTGTGAAGTTCTTCATTTCCTACGCAATTCCAGATGTGTCCAAAATCA
  CGAAAAGCAAGATCAAGAGGGAGAAATACCTCACGCAGAAGTTGCTTCACGAGAGTCACCTCAAAGACCT
  GACCAAAAACATGGGGATCATCGCCGAGAGGATAGGAGGAACGGTTGACAACAGCGTGCGGCCAAAACTC
  GAA
  
  
  Protein Sequence

  >MR211153 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQMMTRKVLLNMELEEDDDEDGDIVLENFDQTIVCPTFGSLENQQDFRTPEFEEFNGKPDSLFFTDGQRR
  IDFILVYEDESKKENNKKGTNEKQKRKRQAYESNLICHGLQLEATRSVSDDKLVFVKVHAPWEVLCTYAE
  IMHIKLPLKPNDLKTRSPFGNLNWFTKVLRVNESVIKPEQEFFTAPFEKSRMNDFYILDRDSFFNPATRS
  RIVYFILSRVKYQVMNNVNKFGINRLVSSGIYKAAFPLHDCRFNYESEDISCPSERYLLYREWAHPRSIY
  KKQPLDLIRKYYGEKIGIYFAWLGYYTQMLLLAAVVGVACFLYGYLDQDNCTWSKEVCDPDIGGQILMCP
  QCDRLCPFWRLNITCESSKKLCIFDSFGTLIFAVFMGVWVTLFLEFWKRRQAELEYEWDTVELQQEEQAR
  PEYEAQCNHVVINEITQEEERIPFTTCGKCIRVTLCASAVFFWILLIIASVIGIIVYRLSVFIVFSTTLP
  KNPNGTDPIQKYLTPQMATSITASIISFIIIMILNTIYEKVAIMITNFELPRTQTDYENSLTMKMFLFQF
  VNYYSSCFYIAFFKGKFVGYPGDPVYLLGKYRSEECDPGGCLLELTTQLTIIMGGKAIWNNIQEVLLPWV
  MNLIGRYKRVSGSEKITPRWEQDYHLQPMGKLGLFYEYLEMIIQFGFVTLFVASFPLAPLLALVNNILEI
  RVDAWKLTTQFRRMVPEKAQDIGAWQPIMQGIAILAVVTNAMIIAFTSDMIPRLVYYWSFSIPPYGDHTY
  YTMDGYINNTLSVFNITDFKNTDKENPYIGLGNYTLCRYRDFRNPPGHPQEYKHNIYYWHVIAAKLAFII
  VMEHIIYSVKFFISYAIPDVSKITKSKIKREKYLTQKLLHESHLKDLTKNMGIIAERIGGTVDNSVRPKL
  E
  
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175344
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175344.4
• RefSeq Size: 5662 bp
• RefSeq ORF: 2736 bp
• Locus ID: 105722
• UniProt ID: Q6P9J9
• Cytogenetics: 15 E3-F1
• MW: 106.3 kDa
• Gene Summary: Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets, osteoblasts and fetal thymocytes. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.[UniProtKB/Swiss-Prot Function]