Lpin2 (NM_001164885) Mouse Tagged ORF Clone

CAT#: MR211083

Lpin2 (Myc-DDK-tagged) - Mouse lipin 2 (Lpin2), transcript variant 1

Product Images

Circular map for MR211083

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lpin2
• Synonyms: 2610511G02Rik; AI481352; AW742896
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211083 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTACCTAGAAGATAACAGTGAAGATGAGAAGACGGTTCAGGAAAGCTCGTTGAGTAAACCTGCAA
  GTGTTTACCATGGCAAGGCCCCTCCTGGGATTCTGTCTCAAACCATGAATTATGTGGGCCAGCTGGCTGG
  GCAGGTGCTCGTCACTGTGAAGGAACTCTACAAGGGCATTAACCAAGCCACGTTGTCTGGATGCATCGAT
  GTGGTCGTGGTGAGGCAGCAGGATGGCTCCTACCAGTGCTCGCCTTTTCACGTACGCTTCGGGAAGCTGG
  GTGTCCTGAGGTCCAAGGAGAAAGTGATTGACATAGAAATCAATGGCAGTGCTGTGGATCTTCACATGAA
  ATTGGGTGATAATGGGGAAGCCTTCTTTGTAGAGGAGACTGAAGAAGAATATGAAAAATTACCTGCTTAT
  CTTGCCACCTCACCAATTCCCACTGAAGACCAGTTCTTTAAACATATTGAAACCCCTTTGGTGAAATCAA
  GTGGAAATGAAAGGCCAGCTCAGAGTTCAGACGTTTCTCACACCTTGGAATCAGAGGCAGTTTTCACTCA
  GAGTTCTGTGAAAAAGAAGAAACGAAGGAGAAAGAAGTGCAAACAGGACAATAGGAAGGAGGAGCAGGCA
  GCTTCCCCTGTTGCAGAAGATGTAGGTGATGTGGGTGTGAGCTCAGATGATGAGAAGAGAGCCCAGGCAG
  CAAGAGGATCTTCAAATGCTTCCTTAAAGGAAGAGGACTACAAGGAGCCTTCACTCTTCCATTCTGGGGA
  TAACTACCCCTTATCTGATGGAGATTGGTCCCCATTAGAAACCACCTACCCTCAGGCTGTGTGCCCCAAG
  AGTGACTCTGAGCTGGAGGTGAAGCCATCTGAGAGCCTCCTCAGATCTGAGCCGCACATGGAGTGGACGT
  GGGGCGGGTTCCCAGAGTCCACCAAGGTCACCAAAAGAGAACGGTATGACTATCATCCAAGGACAGCTAC
  GATTACACCATCAGAGAACACACATTTCAGGGTAATTCCCAGTGAAGACAGCCTCATAAGAGAAGTTGAA
  AAGGATGCTACTGTTGAAGATACTACCTGTACCATAGTGAAACCCAAACCTAGAGCCCTGTGTAAGCAAC
  TGAGTGATGCAGCGTCTACTGAGCTTCCCGAATCACCTCTTGAAGCACCTCAGATTTCATCGTTATTAGA
  TGCAGACCCTGTTCCCAGCCCATCAGCAGAGGCTCCCTCAGAACCCAAACCAGCTGCTAAAGACTCACCA
  ACAAAAAAGAAAGGTGTTCACAAAAGAAGCCAGCACCAGGGACCTGATGACATTTACCTTGATGACTTAA
  AGGCTCTTGAGCCTGAAGTGGCGGCTCTCTATTTCCCTAAAAGTGACACGGATCCAGGTTCCAGGCAGTG
  GCCTGAGTCTGACACATTCTCTGGTTCTCAGTCCCCACAGTCTGTGGGGAGTGCAGCTGCGGACAGTGGC
  ACTGAATGCCTCTCAGACTCTGCCATGGACTTGCCTGATGTAACCCTCTCCCTCTGTGGAGGCCTCAGTG
  AGAATGGAGAGATTTCTAAAGAGAAGTTCATGGAGCATATCATCACTTACCATGAGTTTGCAGAAAACCC
  TGGCCTTATCGACAACCCAAACCTCGTGATCCGGATATATAACCGTTACTACAACTGGGCGTTGGCTGCT
  CCCATGATCCTTAGCTTACAGGTATTTCAGAAGAGTTTGCCTAAGGCCACCGTTGAGTCCTGGGTTAAAG
  ACAAGATGCCAAAGAAATCTGGTCGATGGTGGTTTTGGCGGAAAAAAGAAAGTATGATCAAACAGTTGCC
  AGAGACCAAGGAGGGAAAATCTGAGGTCCCTCCAGCAAATGACCTGCCTTCCAATGCTGAGGAGCCAACC
  AGTGCCAGACCTGCAGAGAATGACACTTCTAGTGACGAGGGGTCACAGGAGTTGGAAGAAAGCATCAAAG
  TTGACCCCATCACCGTAGAGACACTGAGTCACTGTGGGACGGCCTCATATAAGAAGTCTCTCCGACTCTC
  CTCGGACCAGATAGCAAAACTGAAGCTCCATGATGGCCCCAATGACGTGGTATTCAGTATCACAACCCAG
  TATCAGGGCACCTGTCGGTGTGCAGGGACCATCTACCTGTGGAACTGGAATGACAAAGTCATCATCTCTG
  ACATCGACGGAACAATAACCAAGTCTGATGCTTTGGGGCAGATTCTCCCACAGCTGGGTAAAGACTGGAC
  GCATCAGGGCATAGCTAGGCTCTACCATTCCATCAATGAGAATGGCTACAAGTTTCTGTACTGTTCTGCA
  CGTGCCATCGGCATGGCCGACATGACCCGTGGTTATCTGCACTGGGTCAATGATAAGGGGACGATCTTGC
  CTCGAGGCCCTCTGATGCTGTCTCCCAGCAGCTTGTTCTCTGCCTTCCACAGGGAAGTGATAGAAAAGAA
  ACCAGAGAAGTTCAAAATTGAGTGTCTGAATGATATTAAGAACTTGTTTGCCCCGTCCAGGCAGCCCTTC
  TATGCTGCCTTTGGAAACCGTCCCAACGATGTCTATGCTTACACACAAGTCGGAGTTCCAGACTGTAGGA
  TATTTACTGTGAATCCAAAGGGTGAATTAATCCAAGAGAGGACCAAAGGGAACAAATCATCGTATCACAG
  GCTGAGTGAGCTTGTGGAACACGTGTTCCCACTTCTCAGTAAGGAGCAGAATTCTGCCTTTCCATGCCCA
  GAGTTCAGCTCCTTCTGCTACTGGCGAGACCCAATCCCTGACCTGGACCTGGATGACCTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211083 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLYLEDNSEDEKTVQESSLSKPASVYHGKAPPGILSQTMNYVGQLAGQVLVTVKELYKGINQATLSGCID
  VVVVRQQDGSYQCSPFHVRFGKLGVLRSKEKVIDIEINGSAVDLHMKLGDNGEAFFVEETEEEYEKLPAY
  LATSPIPTEDQFFKHIETPLVKSSGNERPAQSSDVSHTLESEAVFTQSSVKKKKRRRKKCKQDNRKEEQA
  ASPVAEDVGDVGVSSDDEKRAQAARGSSNASLKEEDYKEPSLFHSGDNYPLSDGDWSPLETTYPQAVCPK
  SDSELEVKPSESLLRSEPHMEWTWGGFPESTKVTKRERYDYHPRTATITPSENTHFRVIPSEDSLIREVE
  KDATVEDTTCTIVKPKPRALCKQLSDAASTELPESPLEAPQISSLLDADPVPSPSAEAPSEPKPAAKDSP
  TKKKGVHKRSQHQGPDDIYLDDLKALEPEVAALYFPKSDTDPGSRQWPESDTFSGSQSPQSVGSAAADSG
  TECLSDSAMDLPDVTLSLCGGLSENGEISKEKFMEHIITYHEFAENPGLIDNPNLVIRIYNRYYNWALAA
  PMILSLQVFQKSLPKATVESWVKDKMPKKSGRWWFWRKKESMIKQLPETKEGKSEVPPANDLPSNAEEPT
  SARPAENDTSSDEGSQELEESIKVDPITVETLSHCGTASYKKSLRLSSDQIAKLKLHDGPNDVVFSITTQ
  YQGTCRCAGTIYLWNWNDKVIISDIDGTITKSDALGQILPQLGKDWTHQGIARLYHSINENGYKFLYCSA
  RAIGMADMTRGYLHWVNDKGTILPRGPLMLSPSSLFSAFHREVIEKKPEKFKIECLNDIKNLFAPSRQPF
  YAAFGNRPNDVYAYTQVGVPDCRIFTVNPKGELIQERTKGNKSSYHRLSELVEHVFPLLSKEQNSAFPCP
  EFSSFCYWRDPIPDLDLDDLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164885
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 5775 bp
• RefSeq ORF: 2796 bp
• Locus ID: 64898
• Cytogenetics: 17 E1.3
• MW: 103.7 kDa
• Gene Summary: Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A to modulate lipid metabolism.[UniProtKB/Swiss-Prot Function]