Ppp1r10 (NM_001163818) Mouse Tagged ORF Clone

CAT#: MR211070

Ppp1r10 (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory subunit 10 (Ppp1r10), transcript variant 1

Product Images

Circular map for MR211070

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ppp1r10
• Synonyms: 2610025H06Rik; Cat53; D17Ertd808e; Fb19; Pnuts
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211070 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCAGGTCCCATAGACCCCAAAGAACTGCTCAAGGGCCTGGATAGCTTCCTTACCCGGGACGGAG
  AAGTGAAGAGTGTGGATGGAATTTCCAAGATCTTCAGTCTAATGAAGGAGGCACGAAAGATGGTGAGTCG
  GTGCACGTACTTGAACATAATCCTGCAGACCCGCGCTCCAGAAGTGCTCGTCAAGTTTATTGATGTCGGT
  GGCTACAAGCTTCTGAACAACTGGCTAACATATTCAAAGACCACCAACAACATTCCTCTCCTACAGCAGA
  TTCTGCTGACTCTGCAGCACCTCCCGCTCACTGTGGACCATCTCAAGCAGAACAACACAGCAAAACTAGT
  GAAGCAGCTCAGCAAGTCAAGTGAGGATGAAGAGCTCCGGAAATTGGCATCAGTCCTTGTCAGTGACTGG
  ATGGCTGTCATCCGCTCCCAGAGTAGCACCCAGCCTGCAGAGAAAGATAAGAAGAAAAGGAAGGAAGAGG
  GGAAAAGTCGAACCACGCTTCCTGAGCGGCCTTTGACTGAAGTGAAGGCTGAGACCCGGGCTGAGGAAGC
  CCCAGAGAAGAAGAAAGAGAAGCCCAAGTCACTTAGAACTACGGCACCCAGTCATGCCAAGTTCCGTTCT
  ACTGGACTGGAGCTGGACACCCCATCTTTGGTGCCTGTGAAGAAGAACTCCAGCACTGTGGTTGTGTCAG
  ACAAGTACAACCTGAAGCCCATCCCCCTGAAGCGGCAGAGTGCCACAGCTGCTCCAGGTGACGCTGCCCC
  ACCTGCAGAGAAGAAATACAAGCCTCTCAACACAGCCCCCAATACCACCAAAGAAATCAAAGTGAAGATC
  ATCCCTCCACAGCCTATGGAGGGCCTGGGTTTTCTGGATGCTCTCAATTCAGCCCCTGTCCCAGGCATCA
  AAATTAAGAAGAAGAAGAAGGTACTATCGCCGACTGCTGCCAAGCCCAGCCCTTTTGAAGGGAAAACAAG
  CACCGAGCAGAGCACAGCCAAACCTTCCTCCCCAGAGCCTGCACCACCTGCTGAGCCCATGGACACGGAC
  CGCCCTGGCACCCCTGTTCCCCCTGTTGAAGTTCCAGAGCTCATGGATGCAGCCTCTTCAGAGCCAGGAG
  CTCTGGATGCAAAGCCTGTGGACAGCCCTGGAGATCCCAACCAGCTGACTCGCAAGGGCAGAAAGAGGAA
  AACTGTGACTTGGCCTGAGGAGGGCAAGCTGAGAGAGTATTTCTACTTTGAACTGGACGAAACTGAGCGA
  GTGAATGTGAACAAGATCAAAGACTTCGGGGAGGCTGCTAAGCGTGAGATACTGTCTGACCGACATGCTT
  TCGAGACAGCTCGGCGACTAAGCCATGACAATATGGAGGAGAAAGTGCCTTGGGTGTGTCCCAGGCCTCT
  GGTTCTGCCCTCACCTCTTGTCATCCCTGGAAGCAACAGCCAGGAGCGATACATCCAGGCAGAGCGGGAG
  AAGGGCATCCTTCAGGAACTCTTCCTGAACAAGGAGAGTCCACATGAACCTGATCCTGAGCCATATGAAC
  CCATTCCCCCAAAACTCATCCCCTTGGATGAGGAATGTGCCATGGATGAGACTCCATATGTCGAGACCCT
  GGAGCCTGGAGGGTCTGGTGGCTCACCTGATGGAGCAGGAGGCTCCAAGCTGCCTCCAGTTCTGGCCAAT
  CTTATGGGAAGCATGGGAGCTGGAAAGAGCCCCCAGGGCCCTGGAGGAGGCGGCATCAATGTTCAGGAGA
  TCCTCACTTCCATAATGGGCAGTCCAAACAGCCACCCTTCAGAGGAGCTGCTGAAGCAGCCTGACTACTC
  AGACAAGCTCAAGCAGATGCTGGTGCCTCATGGGCTCCTAGGTCCTGGTCCTGTGGCCAATGGCTTTCCA
  CCAGGAGGCCCTGGGGGCCCGAAGGGTATGCAGCATTTCCCCCCTGGTCCTGGAGGGCCCATGCCAGGTC
  CCCATGGAGGCCCTGGTGGACCAGTGGGTCCACGTCTCTTAGGTCCCCCACCACCTTCTCGGGGAGGTGA
  TCCATTCTGGGATGGCCCAGGTGATCCAATGCGAGGTGGCCCAATGCGTGGGGGTCCAGGACCTGCTCCC
  GGACCATACCACAGAGGCCGAGGGGGTCGAGGAGGAAATGAGCCACCGCCACCACCTCCATTCCGAGGAG
  CCAGAGGAGGTCGTTCCGGAGGAGGACCACCAAATGGCCGAGGGGGTCCTGGTGGAGGAGGCATGGTTGG
  AGGTGGTGGGCACCGCCCCCATGAAGGCCCTGGAGGGAGCATGGGCAGTGGACATCGTTCCCATGATGGC
  CCTGGAGGGAACATGGGCAGTGGACATCGTTCTCATGATGGCCCTGGAGGGAACATGGGTGGAAGTGGTG
  GACATCGTTCCCATGAAGGCCCTGGACACGGAGGACCCCATGGACACCGACCACATGATGTCCCTAGCCA
  TCGAGGCCACGACCATCGAGGGCCACCACCTCATGAACACCGTGGTCATGATGGCCATGGGGGAGGCGGC
  CACCGAGGGCATGATGGAGGCCACAGTCATGGAGGAGACATGTCAAACCGCCCTGTCTGCCGACACTTCA
  TGATGAAGGGCAACTGCCGCTATGAGAACAACTGTGCCTTCTACCACCCAGGGGTCAATGGGCCCCCACT
  GCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211070 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSGPIDPKELLKGLDSFLTRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNIILQTRAPEVLVKFIDVG
  GYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDW
  MAVIRSQSSTQPAEKDKKKRKEEGKSRTTLPERPLTEVKAETRAEEAPEKKKEKPKSLRTTAPSHAKFRS
  TGLELDTPSLVPVKKNSSTVVVSDKYNLKPIPLKRQSATAAPGDAAPPAEKKYKPLNTAPNTTKEIKVKI
  IPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAKPSPFEGKTSTEQSTAKPSSPEPAPPAEPMDTD
  RPGTPVPPVEVPELMDAASSEPGALDAKPVDSPGDPNQLTRKGRKRKTVTWPEEGKLREYFYFELDETER
  VNVNKIKDFGEAAKREILSDRHAFETARRLSHDNMEEKVPWVCPRPLVLPSPLVIPGSNSQERYIQAERE
  KGILQELFLNKESPHEPDPEPYEPIPPKLIPLDEECAMDETPYVETLEPGGSGGSPDGAGGSKLPPVLAN
  LMGSMGAGKSPQGPGGGGINVQEILTSIMGSPNSHPSEELLKQPDYSDKLKQMLVPHGLLGPGPVANGFP
  PGGPGGPKGMQHFPPGPGGPMPGPHGGPGGPVGPRLLGPPPPSRGGDPFWDGPGDPMRGGPMRGGPGPAP
  GPYHRGRGGRGGNEPPPPPPFRGARGGRSGGGPPNGRGGPGGGGMVGGGGHRPHEGPGGSMGSGHRSHDG
  PGGNMGSGHRSHDGPGGNMGGSGGHRSHEGPGHGGPHGHRPHDVPSHRGHDHRGPPPHEHRGHDGHGGGG
  HRGHDGGHSHGGDMSNRPVCRHFMMKGNCRYENNCAFYHPGVNGPPLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163818
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163818.1, NP_001157290.1
• RefSeq Size: 4239 bp
• RefSeq ORF: 2667 bp
• Locus ID: 52040
• UniProt ID: Q80W00
• Cytogenetics: 17 18.77 cM
• MW: 94.4 kDa
• Gene Summary: Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.[UniProtKB/Swiss-Prot Function]