Prkd2 (NM_178900) Mouse Tagged ORF Clone

CAT#: MR211037

Prkd2 (Myc-DDK-tagged) - Mouse protein kinase D2 (Prkd2)

Product Images

Circular map for MR211037

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prkd2
• Synonyms: AI325941; PKD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211037 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCGCCCCCTCCCATCCCGCAGGACTCCCCGGCTCTCCGGGGCCCGGGTCTCCTCCGCCCCCCG
  GTGGCTTGGATCTCCAGTCGCCGCCACCTCTGCTGCCTCAGATCCCGGCTCCGGGTTCGGGGGTCTCCTT
  CCATATCCAGATCGGATTGACCCGAGAGTTCGTGCTGTTGCCGGCTGCCTCAGAGTTGGCTCATGTGAAG
  CAACTAGCCTGTTCTATCGTGGACCAGAAGTTCCCAGAGTGTGGCTTCTACGGCCTGTACGACAAGATCC
  TGCTCTTCAAACATGACCCAACATCAGCCAACCTCCTGCAGCTGGTGCGATCAGCTGCAGATATCCAGGA
  GGGTGACCTGGTGGAGGTGGTTCTGTCGGCCTCGGCCACCTTCGAGGATTTCCAGATCCGACCGCATGCT
  CTCACTGTGCACTCGTACCGCGCTCCCGCCTTCTGTGACCACTGCGGGGAGATGCTCTTCGGCCTCGTGC
  GCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAATTACCACAAACGCTGCGCCTTCAGCATCCCGAACAA
  CTGCAGTGGTGCTCGAAAGCGCCGCCTGTCATCCACGTCTCTGGCCAGTGGCCATTCTGTGCGCCTTGGC
  AGCTCCGAGTCCTTACCCTGCACCGCCGAAGAGCTGAGCCGTAGCACCACCGACCTCCTTCCTCGCCGAC
  CACCCTCGTCGTCGTCCTCCTCCTCTTCCTCTTCCTTCTACACAGGCCGACCCATTGAGTTGGACAAGAT
  GCTGATGTCCAAGGTCAAGGTGCCACACACCTTCCTTATACATAGCTACACACGGCCCACCGTTTGCCAG
  GCTTGCAAGAAACTGCTCAAGGGTCTATTCCGCCAGGGCCTGCAGTGCAAAGACTGCAAGTTTAACTGTC
  ACAAACGCTGTGCTACCCGCGTCCCTAACGACTGCCTGGGGGAGGCGCTCATCAATGGAGACGTGCCGAT
  GGAGGAAGCCGCTGATTACAGTGAGGCTGATAAGAGCTCCATCTCAGATGAGTTGGAGGATTCTGGTGTC
  ATCCCCGGCTCCCACTCAGAGAGTGCTCTCCATGCCAGTGAGGAAGAGGAAGGCGAGGGACACAAGGCCC
  AGAGCTCGCTGGGATACATCCCCCTGATGCGCGTGGTACAGTCTGTGCGACACACGACCCGGAAATCCAG
  CACCACCCTGCGGGAGGGCTGGGTGGTCCATTACAGCAACAAGGACACACTGAGGAAACGGCACTACTGG
  CGTCTAGACTGTAAATGCATTACCCTCTTCCAGAACAACACGACCAACAGATACTACAAGGAAATCCCGC
  TGTCTGAAATCCTTGCGGTGGAACCGGCTCAGAACTTCAGCCTCGTACCCCCGGGCACCAACCCACACTG
  CTTCGAGATCATCACGGCCAATGTCACCTACTTTGTGGGCGAAACCCCTGGCGGGGCCCCGGGAGGGCCA
  AGTGGACAGGGAACAGAGGCCGTTCGGGGTTGGGAGACAGCCATCCGCCAGGCACTGATGCCCGTTATCC
  TCCAAGATGCGCCCAGTGCCCCGGGGCACACACCGCACAGACAAGCGTCTCTGAGTATCTCCGTGTCCAA
  CAGTCAGATCCAAGAGAATGTGGATATCGCCACTGTCTACCAGATCTTCCCAGATGAGGTGCTGGGCTCT
  GGGCAGTTTGGAGTGGTTTATGGAGGAAAACACAGGAAGACTGGCAGAGACGTTGCAGTAAAGGTCATTG
  ACAAACTGCGCTTCCCCACCAAGCAGGAGAGCCAGCTCAGGAATGAAGTGGCCATTCTCCAGAGTCTACG
  GCACCCCGGGATTGTGAACCTCGAGTGCATGTTTGAGACTCCTGAGAAGGTATTCGTGGTGATGGAGAAA
  CTGCACGGGGACATGTTGGAGATGATCCTCTCCAGTGAGAAGGGCCGGCTTCCTGAGCGTCTCACCAAGT
  TCCTTATCACGCAGATCCTGGTGGCGCTGAGACACCTGCACTTCAAGAACATCGTCCACTGTGACTTGAA
  ACCAGAGAATGTGTTACTGGCATCAGCCGATCCGTTTCCTCAGGTGAAGCTCTGTGACTTTGGCTTCGCT
  CGCATCATCGGCGAGAAGTCCTTCCGGCGCTCAGTGGTGGGCACGCCTGCCTACCTGGCACCCGAGGTAC
  TGCTCAACCAGGGTTACAACCGCTCGCTGGACATGTGGTCCGTGGGTGTGATCATGTACGTGAGCCTTAG
  TGGCACGTTCCCCTTCAATGAGGACGAGGACATCAATGACCAGATACAGAACGCGGCCTTTATGTACCCG
  GCCAGCCCCTGGAGCCACATCTCATCTGGAGCCATCGACCTCATCAACAACCTGTTGCAGGTGAAGATGC
  GCAAGCGCTACAGCGTGGACAAGTCTCTCAGCCACCCATGGTTACAGGAGTACCAGACGTGGCTTGACCT
  CCGAGAGCTAGAGGGAAAGATGGGCGAGCGATATATCACGCACGAGAGCGACGACGCACGCTGGGATCAG
  TTTGTGGCAGAGCGCCATGGGACTCCTGCAGAAGGGGACCTGGGTGGCGCCTGTCTGCCGCAGGACCACG
  AGATGCAGGGGCTGGCTGAGCGCATCAGCATCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211037 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAPSHPAGLPGSPGPGSPPPPGGLDLQSPPPLLPQIPAPGSGVSFHIQIGLTREFVLLPAASELAHVK
  QLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAADIQEGDLVEVVLSASATFEDFQIRPHA
  LTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLG
  SSESLPCTAEELSRSTTDLLPRRPPSSSSSSSSSSFYTGRPIELDKMLMSKVKVPHTFLIHSYTRPTVCQ
  ACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEAADYSEADKSSISDELEDSGV
  IPGSHSESALHASEEEEGEGHKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYW
  RLDCKCITLFQNNTTNRYYKEIPLSEILAVEPAQNFSLVPPGTNPHCFEIITANVTYFVGETPGGAPGGP
  SGQGTEAVRGWETAIRQALMPVILQDAPSAPGHTPHRQASLSISVSNSQIQENVDIATVYQIFPDEVLGS
  GQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEK
  LHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFA
  RIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYP
  ASPWSHISSGAIDLINNLLQVKMRKRYSVDKSLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWDQ
  FVAERHGTPAEGDLGGACLPQDHEMQGLAERISIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178900
• ORF Size: 2628 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178900.4, NP_849231.1
• RefSeq Size: 3625 bp
• RefSeq ORF: 2628 bp
• Locus ID: 101540
• UniProt ID: Q8BZ03
• Cytogenetics: 7 A2
• MW: 96.5 kDa
• Gene Summary: Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of cell proliferation via MAPK1/3 (ERK1/2) signaling, oxidative stress-induced NF-kappa-B activation, inhibition of HDAC7 transcriptional repression, signaling downstream of T-cell antigen receptor (TCR) and cytokine production, and plays a role in Golgi membrane trafficking, angiogenesis, secretory granule release and cell adhesion (PubMed:17226786, PubMed:20819079). May potentiate mitogenesis induced by the neuropeptide bombesin by mediating an increase in the duration of MAPK1/3 (ERK1/2) signaling, which leads to accumulation of immediate-early gene products including FOS that stimulate cell cycle progression (PubMed:17226786). In response to oxidative stress, is phosphorylated at Tyr-438 and Tyr-718 by ABL1, which leads to the activation of PRKD2 without increasing its catalytic activity, and mediates activation of NF-kappa-B (By similarity). In response to the activation of the gastrin receptor CCKBR, is phosphorylated at Ser-244 by CSNK1D and CSNK1E, translocates to the nucleus, phosphorylates HDAC7, leading to nuclear export of HDAC7 and inhibition of HDAC7 transcriptional repression of NR4A1/NUR77 (By similarity). Upon TCR stimulation, is activated independently of ZAP70, translocates from the cytoplasm to the nucleus and is required for interleukin-2 (IL2) promoter up-regulation. During adaptive immune responses, is required in peripheral T-lymphocytes for the production of the effector cytokines IL2 and IFNG after TCR engagement and for optimal induction of antibody responses to antigens (PubMed:20819079). In epithelial cells stimulated with lysophosphatidic acid (LPA), is activated through a PKC-dependent pathway and mediates LPA-stimulated interleukin-8 (IL8) secretion via a NF-kappa-B-dependent pathway (By similarity). During TCR-induced T-cell activation, interacts with and is activated by the tyrosine kinase LCK, which results in the activation of the NFAT transcription factors (By similarity). In the trans-Golgi network (TGN), regulates the fission of transport vesicles that are on their way to the plasma membrane and in polarized cells is involved in the transport of proteins from the TGN to the basolateral membrane (By similarity). Plays an important role in endothelial cell proliferation and migration prior to angiogenesis, partly through modulation of the expression of KDR/VEGFR2 and FGFR1, two key growth factor receptors involved in angiogenesis (By similarity). In secretory pathway, is required for the release of chromogranin-A (CHGA)-containing secretory granules from the TGN (By similarity). Downstream of PRKCA, plays important roles in angiotensin-2-induced monocyte adhesion to endothelial cells (By similarity).[UniProtKB/Swiss-Prot Function]