Copg2 (NM_017478) Mouse Tagged ORF Clone

CAT#: MR211019

Copg2 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit gamma 2 (Copg2)

Product Images

Circular map for MR211019

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Copg2
• Synonyms: AW227625; gamma-2-COP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211019 representing NM_017478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTAAAAAATTCGACAAGAAGGACGAGGAGTCTGGTAGTGGTTCCAATCCTTTCCAGCATTTGGAGA
  AGAGTGCTGTCTTGCAGGAGGCACGCATATTCAACGAAACGCCCATCAATCCAAGAAGATGCTTGCATAT
  CTTGACAAAGATTCTTTACTTACTCAACCAGGGTGAACACTTCGGAACTATGGAAGCTACAGAAGCCTTC
  TTTGCAATGACTCGATTGTTTCAATCTAATGATCAAACGCTGAGGCGGATGTGCTACCTCACCATCAAGG
  AAATGGCCACCATCTCTGAGGATGTGATCATTGTCACAAGCAGTCTGACCAAGGACATGACTGGAAAGGA
  AGATGTATACCGAGGCCCAGCCATCAGGGCTCTCTGCAGAATCACAGATGGAACAATGTTGCAAGCCGTT
  GAAAGGTACATGAAGCAGGCCATTGTGGATAAAGTATCCAGTGTAGCCAGCTCAGCGCTGGTCTCTTCCT
  TACACATGATGAAGATAAGCTATGATGTGGTTAAACGATGGATAAATGAAGCCCAAGAAGCTGCATCAAG
  TGACAATATCATGGTGCAGTACCATGCACTAGGAGTGCTCTATCACCTTAGGAAGAATGATCGGCTTGCT
  GTCTCCAAGATGTTAAATAAGTTTACCAAATCTGGTCTCAAATCACAGTTTGCATACTGTATGCTGATTC
  GAATTGCTAGTCGCTTACTGAAAGAAAGCGAGGATGGTCATGAAAGTCCACTCTTTGATTTCATTGAGAG
  CTGCTTGCGAAATAAACACGAAATGGTTATTTATGAAGCTGCTTCTGCCATTATTCATCTTCCCAACTGT
  ACTGCAAGAGAGCTGGCACCTGCTGTCTCAGTTCTTCAACTTTTCTGTAGCTCTCCTAAACCAGCCTTGA
  GATATGCAGCTGTGAGGACCCTGAACAAGGTGGCAATGAAGCACCCCTCTGCTGTTACTGCCTGCAATCT
  GGACTTAGAAAACTTAATCACAGACTCAAACAGGAGCATTGCTACCCTGGCCATTACAACATTGCTCAAA
  ACAGGAAGCGAGAGCAGTGTAGACAGGCTTATGAAGCAGATATCTTCTTTTGTGTCTGAAATCTCAGATG
  AGTTCAAGGTGGTGGTCGTGCAGGCCATCAGTGCTCTCTGTCACAAGTACCCTCGGAAGCACAGCGTCAT
  GATGACTTTTCTTTCCAACATGCTTCGAGATGATGGAGGTTTTGAATACAAAAAGGCCATTGTGGACTGC
  ATAATCAGCATTGTGGAAGAGAACCCTGAGAGTAAAGAAGCTGGTCTAGCCCACCTCTGTGAATTCATTG
  AGGACTGTGAACATACCGTTCTGGCTACTAAAATTCTCCACTTATTGGGCAAAGAGGGCCCTAGAACTCC
  TGTTCCCTCCAAGTATATCAGATTCATTTTTAATAGGGTGGTCCTGGAGAATGAGGCTGTCAGAGCTGCT
  GCTGTGAGTGCTTTGGCTAAATTTGGGGCTCAGAATGAGAGCCTTCTCCCCAGCATCCTTGTACTCTTAC
  AAAGGTGTATGATGGATACTGATGATGAGGTACGGGACCGAGCTACCTTCTACCTGAATGTGCTACAGCA
  GAGACAGATGGCCCTGAATGCTACATATATCTTCAATGGTCTGACAGTCTCTATACCAGGGATGGAAAAG
  GCCTTACACCAGTATACATTGGAGCCTTCAGAAAAACCCTTTGATATGAAGTCAATTCCTCTTGCTATGG
  CTCCTGTCTTTGAGCAGAAGTCAGAAATTACACTTGTGACTCCTAAGCCAGAGAAGTTGGCTCCTTCCAG
  GCAAGACATTTTCCAAGAACAGTTAGCTGCCATTCCTGAGTTTATGAACCTGGGACCCTTGTTTAAGTCT
  TCTGAGCCTGTGCAGCTTACAGAAGCAGAGACAGAGTATTTCGTTCGCTGTGTGAAGCACATGTTTACTG
  ACCATATTGTATTCCAGTTTGACTGTACCAACACTCTAAATGACCAGCTGCTGGAGAAAGTAACAGTGCA
  GATGGAACCATCCGATTCCTATGAAGTGCTGTGCTGTATCCCAGCTCCCAGCCTCCCTTACAATCAGCCA
  GGAATATGCTACACACTTGTTCGATTGCCAGATGAAGATCCTACAGCAGTTGCAGGCACCTTTAGCTGTA
  CCATGAAGTTCACAGTCCGGGATTGTGACCCTAATACTGGAGTTCCAGATGAAGATGGATATGATGATGA
  ATATGTGCTGGAAGATCTTGAAGTAACTGTGTCTGATCATATTCAGAAGATACTAAAACCTAACTTCGCT
  GCTGCCTGGGAAGAGGTGGGAGATGCCTTTGAAAAAGAAGAAACCTTTGCCCTGAGCTCTACAAAAACCC
  TTGAAGAAGCTGTCAACAACATCATCACATTTCTTGGTATGCAGCCATGTGAGAGGTCAGATAAAGTCCC
  CGAGAATAAGAACTCCCACTCCCTCTATCTAGCAGGTGTATACAGAGGTGGCTATGACTTATTGGTGCGG
  TCCAGGCTGGCCCTAGCTGATGGAGTGACCATGCAGGTGACTGTGAGAAGCAAAGAGAGAACGCCTGTGG
  ATGTTATCTTGGCTTCTGTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211019 representing NM_017478
  Red=Cloning site Green=Tags(s)
  
  MIKKFDKKDEESGSGSNPFQHLEKSAVLQEARIFNETPINPRRCLHILTKILYLLNQGEHFGTMEATEAF
  FAMTRLFQSNDQTLRRMCYLTIKEMATISEDVIIVTSSLTKDMTGKEDVYRGPAIRALCRITDGTMLQAV
  ERYMKQAIVDKVSSVASSALVSSLHMMKISYDVVKRWINEAQEAASSDNIMVQYHALGVLYHLRKNDRLA
  VSKMLNKFTKSGLKSQFAYCMLIRIASRLLKESEDGHESPLFDFIESCLRNKHEMVIYEAASAIIHLPNC
  TARELAPAVSVLQLFCSSPKPALRYAAVRTLNKVAMKHPSAVTACNLDLENLITDSNRSIATLAITTLLK
  TGSESSVDRLMKQISSFVSEISDEFKVVVVQAISALCHKYPRKHSVMMTFLSNMLRDDGGFEYKKAIVDC
  IISIVEENPESKEAGLAHLCEFIEDCEHTVLATKILHLLGKEGPRTPVPSKYIRFIFNRVVLENEAVRAA
  AVSALAKFGAQNESLLPSILVLLQRCMMDTDDEVRDRATFYLNVLQQRQMALNATYIFNGLTVSIPGMEK
  ALHQYTLEPSEKPFDMKSIPLAMAPVFEQKSEITLVTPKPEKLAPSRQDIFQEQLAAIPEFMNLGPLFKS
  SEPVQLTEAETEYFVRCVKHMFTDHIVFQFDCTNTLNDQLLEKVTVQMEPSDSYEVLCCIPAPSLPYNQP
  GICYTLVRLPDEDPTAVAGTFSCTMKFTVRDCDPNTGVPDEDGYDDEYVLEDLEVTVSDHIQKILKPNFA
  AAWEEVGDAFEKEETFALSSTKTLEEAVNNIITFLGMQPCERSDKVPENKNSHSLYLAGVYRGGYDLLVR
  SRLALADGVTMQVTVRSKERTPVDVILASVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017478
• ORF Size: 2613 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017478.3, NP_059506.1
• RefSeq Size: 4016 bp
• RefSeq ORF: 2616 bp
• Locus ID: 54160
• UniProt ID: Q9QXK3
• Cytogenetics: 6 A3.3
• MW: 97.7 kDa
• Gene Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function]