Mastl (NM_025979) Mouse Tagged ORF Clone

CAT#: MR211003

Mastl (Myc-DDK-tagged) - Mouse microtubule associated serine/threonine kinase-like (Mastl)

Product Images

Circular map for MR211003

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mastl
• Synonyms: 2700091H24Rik; C88295; GW; GWL; MAST-L; THC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211003 representing NM_025979
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCCGCCTCGGCAAGTGAGGAGAATGAAGGAGGTGCGGCGATAGAGGAGTGCGTGAGTAGGATCC
  CGGTACCAAGACCACCCTCCATTGAGGAGTTCACCATAGTGAAGCCCATTAGCCGTGGTGCATTCGGGAA
  AGTGTACCTGGGACAGAAAGGCGGCAAGTTGTACGCAGTGAAGGTTGTCAAAAAGGCAGACATGATCAAT
  AAAAATATGACTCATCAGGTACAAGCTGAGAGAGATGCCCTGGCACTGAGCAAAAGCCCTTTTGTTGTGC
  ATCTCTATTACTCACTGCAGTCAGCAAGCAACATCTACTTGATAATGGAGTATCTTATTGGTGGAGATGT
  CAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTATAAAATATATTTCTGAAGTAGCA
  TTGGCTTTAGACTACCTGCACAGACATGGAATCATCCATAGGGATTTGAAACCGGACAATATGCTTATTT
  CCAATGAGGGTCATATTAAACTGACAGATTTTGGCCTTTCCAAAGTTACTTTGAATAGAGACATCAATAT
  GATGGATATTCTCACAACACCATCAATGTCTAAACCTAAGCAAGATTATTCAAGAACTCCAGGACAAGTC
  TTATCTCTCATCAGTTCTTTGGGATTTTTCACACCAGTTGGAGAAAAAGATCAAGACTCTGCAAACATGT
  TTTCAGCCCCTAAGTCTGCTGCACAACTTTCTCGAGGATTCATTTGTCCTATGTCTGTAGATCAGAAGGA
  GCCGACTTCTTATTCGAGCAAACTGCTAAAATCATGTTTTGAAACCCTTTCATCCAATCCAGAAATACCT
  GTGAAATGTCTGACTTCTAATCTACTCCAGTGTAGGAAAAGACTGGGTACCTCAAGTACTAGTAGCCAGT
  CTCATACCTTCGTATCCAGTGTGGAGTCAGAATGCCACAGCAATCCCAAATGGGAAAGAGACTGCCAGAG
  TACCGAATCATCAGGCTGTGCAATGAGCTGGAATGCAGTTGAAATGCTGTATGCAAAATCTACAAGTGCC
  ATCAAGACAAAAACAGAACTGGAATTAGCACTTTCTCCCATTCATGACAGCAGTGCCATTCCTGCTGCTG
  GAAGCAACCAGGTGACCCTCCCTAGAAAATGCTTCAGGGAAATCTCTTGGGAAGCAAGGGACCCAGATAA
  TGAGAACATGACCATTGACAAGGGACAGTCTGGTTTCTGTCAGTCCAGTCAGAGGTCTGTAAATTCTAGT
  GCTACATCTGAAGAACACCTTGGTAAAAGAAATTATAAAAGAAATTTCCACTTGGTTGACTCCAGTCCTT
  GTCAGGAAATTATGCAAAGTAAAAAAAACTGTACAGAGTATGAGGCCAATAAGGAAAGGCAAGGCTGCCG
  TGCAAATCAAAGTACAGGCTTGACAACCGAAGTCCAGAACCTGAAGCTATCAGGGTGTGAAAGTCAGCAA
  CTTGACTATGCCAATAAGGAGAACATTGTCACCTATTTAACTGACAGACAAACCCCAGAAAAACTGCATA
  TACCAACGATAGCAAAGAACCTTATGTCTGAACTAGATGAAGACTGTGAGCTGAGCAGTAAAAAGGACTG
  CCTCAGTTCTAATTCTGTATGTTCTGATGAGGATAGAGCTCTTAAAACTACCTGTGTGGATTCGGATTCA
  TCTTTTCCTGGAGTGTCTATGATGGAGAGTTCACTGGAAATTCAGGCCTTAGAACCAGATAAAAGCATCA
  GAGACTACTCTTTTGAAGAACCAAACACTGAAGATCTATTTGTGTTACCAAAATGCCAAGAAAATTCCTT
  GCCACAAGATGACTGCCATGCTTGTATCCAAGACAGTAGCCAAGTGTCAGCTCATCCCTCAAAGGCGCCC
  AAAGCATTGACCTCTAAAATCAACGTCGTGGCTTTTCGAAGTTTTAACAGTCATATTAACGCATCTACTA
  ATTCGGAACCATCCAAAATCAGCATAACTTCTTTAGATGCTATGGACATTTCCTGTGATTACAGTGGTTC
  ATACCCCATGGCTGTGAGCCCTACTGAGAAAGGAAGGCACTACACGTCACATCAGACTCCAAATCAGGTC
  AAGTTGGGAACATCATACAGAACTCCAAAGAGTGTGAGAAGAGGGGCAGCCCCAGTAGATGACGGGCGAA
  TTCTAGGGACTCCAGATTACCTGGCACCTGAGCTCTTACTGGGTACAGCCCATGGTCCTGCAGTAGACTG
  GTGGGCTCTTGGAGTTTGCTTGTTCGAATTTCTGACAGGAATTCCCCCTTTCAATGATGAAACACCACAA
  CAAGTATTCCAGAATATTTTAAAAAGAGATATCCCATGGCCAGAAGGCGAAGAAAAGCTATCTGATAATG
  CTCAAAGTGCAATGGACATGCTTTTAACCATTGATGATTCAAAGAGAGCTGGAATGAGAGAACTAAAACA
  GCATCCTCTCTTCAGTGAAGTGGACTGGGAAAATCTGCAGCATCAGACTATGCCTTTCGTACCCCAACCA
  GACGACGAAACAGATACATCCTATTTTGAAGCCAGAAATAATGCTCAACATCTGACCATATCTGGGTTTA
  GTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211003 representing NM_025979
  Red=Cloning site Green=Tags(s)
  
  MESASASEENEGGAAIEECVSRIPVPRPPSIEEFTIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMIN
  KNMTHQVQAERDALALSKSPFVVHLYYSLQSASNIYLIMEYLIGGDVKSLLHIYGYFDEEMAIKYISEVA
  LALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMSKPKQDYSRTPGQV
  LSLISSLGFFTPVGEKDQDSANMFSAPKSAAQLSRGFICPMSVDQKEPTSYSSKLLKSCFETLSSNPEIP
  VKCLTSNLLQCRKRLGTSSTSSQSHTFVSSVESECHSNPKWERDCQSTESSGCAMSWNAVEMLYAKSTSA
  IKTKTELELALSPIHDSSAIPAAGSNQVTLPRKCFREISWEARDPDNENMTIDKGQSGFCQSSQRSVNSS
  ATSEEHLGKRNYKRNFHLVDSSPCQEIMQSKKNCTEYEANKERQGCRANQSTGLTTEVQNLKLSGCESQQ
  LDYANKENIVTYLTDRQTPEKLHIPTIAKNLMSELDEDCELSSKKDCLSSNSVCSDEDRALKTTCVDSDS
  SFPGVSMMESSLEIQALEPDKSIRDYSFEEPNTEDLFVLPKCQENSLPQDDCHACIQDSSQVSAHPSKAP
  KALTSKINVVAFRSFNSHINASTNSEPSKISITSLDAMDISCDYSGSYPMAVSPTEKGRHYTSHQTPNQV
  KLGTSYRTPKSVRRGAAPVDDGRILGTPDYLAPELLLGTAHGPAVDWWALGVCLFEFLTGIPPFNDETPQ
  QVFQNILKRDIPWPEGEEKLSDNAQSAMDMLLTIDDSKRAGMRELKQHPLFSEVDWENLQHQTMPFVPQP
  DDETDTSYFEARNNAQHLTISGFSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025979
• ORF Size: 2595 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025979.4, NP_080255.3
• RefSeq Size: 4652 bp
• RefSeq ORF: 2598 bp
• Locus ID: 67121
• UniProt ID: Q8C0P0
• Cytogenetics: 2 A3
• MW: 96.4 kDa
• Gene Summary: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity).[UniProtKB/Swiss-Prot Function]