Tmc6 (NM_145439) Mouse Tagged ORF Clone

CAT#: MR210775

Tmc6 (Myc-DDK-tagged) - Mouse transmembrane channel-like gene family 6 (Tmc6), transcript variant 1

Product Images

Circular map for MR210775

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmc6
• Synonyms: D11Ertd204e; EVER1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210775 representing NM_145439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGTCACTGGCTTTAGCCCTCGATGTCCCGGAGACCACGGGAGACGAAGGCCTGGAGCCCAGCC
  CCTATGAAGAAAGTGAAGTCCATGACTCCTTCCACCAGCTCATTCAAGAGCAAAGCCTTCGGGTGGCGGA
  GGAGGGGCTGGAGCTACTGCCCTTGGGTCTAGGCAGAGGTGACCAGACCCTCCCAGGACTTGAAGGTGCT
  CCTGCCCTTAGCTCGGCCACTCTCCGCATCCTGGCCAGCATGCCCAGCCGTACCATTGGTCGCAGCCGTG
  GGGCCATCATCTCCCAATACTACAACCGCACAGTGAGGCTTCGGCGCAGGAGCAGCCGGCCCTTGCTGGG
  CAATGTGGTGCCCTCTGCCCGGCCCAGCCTTCGCCTGTATGACCTGGAGCTGGACTCCACAATCTTGGAG
  GAGGATGAGAAGCGGAGCCTGCTAGTAAAGGAGCTTCAAGGTCTGTCGGCGGCCCAGAGGGACCACATGG
  TGCGGAACATGCCCTTGAGTCTGGGTGAGAAGCGCTGTCTTCGAGAGAAAAGTTGGAGCCCAAAGGGAAA
  GCGGCGGCACCTGCAGGGTCGAAGCGGGGCCTTCTCCTGCTGTAGCCGGCTCAGATACACCTGCATGCTG
  GCTCTTCATAGCCTGGGGCTGGCACTGCTCTCAGGCCTGTATGCTGCCAGGCCGTGGCGCTATGCTCTGA
  AGCAGATCGGTGGCCAGTTTGGCTCCAGCGTCCTCTCCTACTTCCTCTTCCTCAAGACCCTGTTGGCCTT
  CAACGCGCTGATGTTGCTGCCTTTGCTGGCCTTCCTCGTGGGTGTTCAGGCTGCCTTTCCACCTGACCCA
  GCAGGCCCCGTGCCTACGTTTTCTGGTCTGGAACTCCTCACAGGCGGGGGCCGGTTCACACACACAGTTA
  TGTACTACGGCTACTACAGTAACAGCACGCTGAGCCCGTCATGTGACGCCCCTCGGGAAGGTGGCCAGTG
  CAGTCCCAGGCTGGGCAGCCTGCCCTATAACATGCCGCTGGCCTACCTCTTCACAATGGGGGCCACCTTC
  TTCCTTACCTGCATCATTCTGGTATACAGCATGTCCCACTCCTTTGGGGAGAGCTACCGGGTTGGCAGTA
  CCAAGGGTATCCATGCCCTTACGGTCTTCTGCTCCTGGGACTACAAGGTGACACAGAAGAGGGCTTCCCG
  TGTCCAACAGGACAGCATCTGCACTCAGCTGAAGGAGCTGTTGGCTGAATGGCACCTTCGAAAGCGCCCT
  CGGAGTGTGTGCGGGCAGCTGAGGCAGGTCGTCGTTCTAGGTCTGGGGTGGCTGCTGTGTCTGGGCTCCA
  CAATGGGCTGCACGGTGGCTGTCCTCACCTTCTCAGAGGTAATGATTCAGAGACCTGCTTCTGGTGGCCA
  GGGGGTGGAGGCGTTGGCCTTGCCCCTGGTGGTCAGTGTCCTTAACCTGGGTGCCTCCTACCTGTTCCGT
  GGTCTAGCCACTCTGGAGCGACATGACTCCCCTGTGTTGGAGGTATACATGGCCATCTGCAGGAACCTCA
  TCCTGAAGATGGCCGTCCTGGGTGTGCTTTGCTATCACTGGCTGGGCCGCAGGGTGGCCACCCTGCAGGG
  TCAGTGCTGGGAGGACTTTGTGGGCCAGGAGCTGTACCGCTTCATGGTCGTGGATTTCATCTTCATGCTC
  TTGGACTCCCTTTTTGGAGAGTTGGTGTGGAGGCTCATCTCAGAGAAGAAGCTCAAGAGGGGGCAGAAGC
  CTGAGTTTGACATTGCCAGGAATGTGCTGGACCTGATTTATGGACAGACACTGACCTGGCTGGGCGTCCT
  GTTCTCACCGCTCCTGCCTGCAGTACAGATTCTCCGGCTGCTCTTCCTTTTCCACATCAAGAAGGCCAGC
  CTGATGGCCAACTGCCAGGCACCACGCCGACCCTGGCTGGCCTCGCACATGAGCACTGTCTTCCTCACCT
  TGCTCTGCTTCCCGTCGTTCCTGGGCGCTGCTGTTTTCCTCTGCTATGCTGTCTGGCAGGTGAGGCCCTC
  GAGCACTTGTGGCCCTTTCCGGACTCTGAACACCATGTATGAAGCAGGCACGGTCTGGGTGCGTCGCCTG
  GAGCATGCAGGCTCCGGAGCCTCCTGGCTGCCCTGGCTGCACCACTTCCTGGTGGAGAACACTTTCTTCC
  TCTTCCTGGCTTCAGCCCTGCTGCTGGCTGTCATCTACTTCAACATCCAGGTGGTGAAAGGACAACGGAA
  GGTCATCTGCCTGCTCAAGGAGCAGATCCGGAATGAAGGAGAGGACAAGATCTTCCTGATCAACAAGCTT
  CACTCTGTTTACGAGGAGGAGGGAAGGAGCAGGCCTGGCAGAACCCAGGACACTACTGAACCACCTGCCT
  GGCATGAGGATGGAGGGGACCAGAAGGAACCCTGTAACCCCCGGTCACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210775 representing NM_145439
  Red=Cloning site Green=Tags(s)
  
  MAQSLALALDVPETTGDEGLEPSPYEESEVHDSFHQLIQEQSLRVAEEGLELLPLGLGRGDQTLPGLEGA
  PALSSATLRILASMPSRTIGRSRGAIISQYYNRTVRLRRRSSRPLLGNVVPSARPSLRLYDLELDSTILE
  EDEKRSLLVKELQGLSAAQRDHMVRNMPLSLGEKRCLREKSWSPKGKRRHLQGRSGAFSCCSRLRYTCML
  ALHSLGLALLSGLYAARPWRYALKQIGGQFGSSVLSYFLFLKTLLAFNALMLLPLLAFLVGVQAAFPPDP
  AGPVPTFSGLELLTGGGRFTHTVMYYGYYSNSTLSPSCDAPREGGQCSPRLGSLPYNMPLAYLFTMGATF
  FLTCIILVYSMSHSFGESYRVGSTKGIHALTVFCSWDYKVTQKRASRVQQDSICTQLKELLAEWHLRKRP
  RSVCGQLRQVVVLGLGWLLCLGSTMGCTVAVLTFSEVMIQRPASGGQGVEALALPLVVSVLNLGASYLFR
  GLATLERHDSPVLEVYMAICRNLILKMAVLGVLCYHWLGRRVATLQGQCWEDFVGQELYRFMVVDFIFML
  LDSLFGELVWRLISEKKLKRGQKPEFDIARNVLDLIYGQTLTWLGVLFSPLLPAVQILRLLFLFHIKKAS
  LMANCQAPRRPWLASHMSTVFLTLLCFPSFLGAAVFLCYAVWQVRPSSTCGPFRTLNTMYEAGTVWVRRL
  EHAGSGASWLPWLHHFLVENTFFLFLASALLLAVIYFNIQVVKGQRKVICLLKEQIRNEGEDKIFLINKL
  HSVYEEEGRSRPGRTQDTTEPPAWHEDGGDQKEPCNPRSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145439
• ORF Size: 2430 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145439.3
• RefSeq Size: 2913 bp
• RefSeq ORF: 2433 bp
• Locus ID: 217353
• UniProt ID: Q7TN60
• Cytogenetics: 11 82.96 cM
• MW: 91 kDa
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]