Tlr6 (NM_011604) Mouse Tagged ORF Clone

CAT#: MR210761

Tlr6 (Myc-DDK-tagged) - Mouse toll-like receptor 6 (Tlr6)

Product Images

Circular map for MR210761

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tlr6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210761 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAAGTCCCTCTGGGATAGCCTCTGCAACATGAGCCAAGACAGAAAACCCATCGTGGGGAGTTTCC
  ACTTTGTTTGCGCCCTGGCCTTAATAGTCGGAAGCATGACCCCGTTCTCTAATGAACTTGAGTCTATGGT
  AGACTATTCAAACAGGAACCTTACTCATGTCCCCAAAGACCTGCCACCAAGAACAAAAGCCCTGAGTCTG
  TCTCAAAACTCTATATCTGAGCTTCGGATGCCTGATATCAGCTTTCTGTCAGAGCTGAGAGTTCTGAGAC
  TCTCCCACAACAGGATACGGAGCCTTGATTTCCATGTATTCTTGTTCAATCAGGACTTAGAATACCTGGA
  TGTCTCACACAATCGGTTGCAAAACATCTCTTGCTGCCCTATGGCGAGCCTGAGGCATCTAGACCTCTCA
  TTCAATGACTTTGATGTACTGCCTGTGTGTAAGGAATTTGGCAACCTGACGAAGCTGACTTTCCTGGGAT
  TAAGTGCTGCAAAGTTCCGACAACTGGATCTGCTCCCAGTTGCTCACTTGCATCTAAGCTGCATTCTTCT
  GGACTTAGTGAGTTATCATATAAAAGGCGGGGAAACAGAAAGTCTTCAGATTCCCAATACCACCGTTCTC
  CATTTGGTCTTTCATCCAAATAGCTTGTTCTCTGTTCAAGTGAACATGTCTGTAAACGCTTTAGGACATT
  TACAACTGAGTAATATTAAATTGAATGATGAAAACTGTCAAAGGTTAATGACATTTTTATCAGAACTCAC
  CAGAGGTCCAACCTTATTGAATGTGACCCTCCAGCACATAGAAACAACCTGGAAGTGCTCGGTTAAACTT
  TTCCAATTCTTTTGGCCCCGACCGGTGGAGTACCTCAATATTTACAACTTAACGATAACTGAGAGAATCG
  ACAGGGAAGAATTTACTTACTCGGAGACAGCACTGAAGTCACTGATGATAGAGCACGTCAAAAACCAAGT
  GTTCCTCTTTTCAAAGGAGGCGCTATACTCGGTGTTTGCTGAGATGAACATCAAGATGCTCTCTATCTCA
  GACACCCCTTTCATCCACATGGTGTGCCCGCCATCCCCAAGCTCATTTACATTTCTGAACTTTACCCAGA
  ATGTTTTTACTGACAGTGTTTTTCAAGGCTGTTCCACCTTAAAGAGATTGCAGACACTTATCTTACAAAG
  GAATGGTTTGAAGAACTTTTTTAAAGTAGCTCTCATGACTAAGAATATGTCCTCTCTGGAAACTTTGGAT
  GTTAGTTTGAATTCTTTGAACTCTCATGCATATGACAGGACATGCGCCTGGGCTGAGAGCATATTGGTGT
  TGAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCAGATGCTTACCTCCCAAGGTCAAGGTCCTTGA
  CCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGTCACCCACCTGCAGGCTTTGCAGGAACTCAAT
  GTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGTGGGGCCTTCAGCAGCCTTTCTGTGCTGGTCATCG
  ACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCCAGAGCTGTCAGAATATTAGATCCCTAACAGC
  GGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGACTTTGTCAAGAACATAGGCTGGGTAGCAAGA
  GAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGACTACCCAGAAAGCTCTAAGGGAACTGCACTGA
  GGGACTTCCACATGTCTCCACTGTCCTGTGATACTGTTCTGCTGACTATCACCATCGGGGCCACTATGCT
  GGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTTTGACCTGCCCTGGTATGTGAGGATGCTGTGT
  CAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCCTTAGAGGAACTCCAGAGAAACCTCCAGTTCC
  ATGCTTTTGTCTCATACAGTGAGCATGATTCTGCCTGGGTGAAGAACGAATTACTACCCAACCTAGAGAA
  AGATGACATCCGGGTTTGCCTCCATGAGAGGAACTTTGTCCCTGGCAAGAGCATTGTGGAGAACATCATC
  AATTTCATTGAGAAGAGTTACAAGGCCATCTTTGTGCTGTCTCCCCACTTCATCCAGAGTGAGTGGTGCC
  ATTATGAACTCTATTTTGCCCATCATAATCTCTTCCATGAAGGCTCTGATAACTTAATCCTCATCTTGCT
  GGAACCCATTCTACAGAACAACATTCCCAGTAGATACCACAAGCTGCGGGCTCTCATGGCACAGCGGACT
  TACTTGGAATGGCCTACTGAGAAGGGCAAACGTGGGCTGTTTTGGGCCAACCTTAGAGCTTCATTTATTA
  TGAAGTTAGCCTTAGTCAATGAGGATGATGTGAAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210761 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVKSLWDSLCNMSQDRKPIVGSFHFVCALALIVGSMTPFSNELESMVDYSNRNLTHVPKDLPPRTKALSL
  SQNSISELRMPDISFLSELRVLRLSHNRIRSLDFHVFLFNQDLEYLDVSHNRLQNISCCPMASLRHLDLS
  FNDFDVLPVCKEFGNLTKLTFLGLSAAKFRQLDLLPVAHLHLSCILLDLVSYHIKGGETESLQIPNTTVL
  HLVFHPNSLFSVQVNMSVNALGHLQLSNIKLNDENCQRLMTFLSELTRGPTLLNVTLQHIETTWKCSVKL
  FQFFWPRPVEYLNIYNLTITERIDREEFTYSETALKSLMIEHVKNQVFLFSKEALYSVFAEMNIKMLSIS
  DTPFIHMVCPPSPSSFTFLNFTQNVFTDSVFQGCSTLKRLQTLILQRNGLKNFFKVALMTKNMSSLETLD
  VSLNSLNSHAYDRTCAWAESILVLNLSSNMLTGSVFRCLPPKVKVLDLHNNRIMSIPKDVTHLQALQELN
  VASNSLTDLPGCGAFSSLSVLVIDHNSVSHPSEDFFQSCQNIRSLTAGNNPFQCTCELRDFVKNIGWVAR
  EVVEGWPDSYRCDYPESSKGTALRDFHMSPLSCDTVLLTITIGATMLVLAVTGAFLCLYFDLPWYVRMLC
  QWTQTRHRARHIPLEELQRNLQFHAFVSYSEHDSAWVKNELLPNLEKDDIRVCLHERNFVPGKSIVENII
  NFIEKSYKAIFVLSPHFIQSEWCHYELYFAHHNLFHEGSDNLILILLEPILQNNIPSRYHKLRALMAQRT
  YLEWPTEKGKRGLFWANLRASFIMKLALVNEDDVKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011604
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011604.2
• RefSeq Size: 2600 bp
• RefSeq ORF: 2421 bp
• Locus ID: 21899
• UniProt ID: Q9EPW9
• Cytogenetics: 5 33.54 cM
• MW: 92.4 kDa
• Gene Summary: Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).[UniProtKB/Swiss-Prot Function]