Tlr6 (NM_011604) Mouse Untagged Clone

CAT#: MC204742

Tlr6 (untagged) - Mouse toll-like receptor 6 (Tlr6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tlr6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055366
  AAGAGGTAATCCGAAGGAAGTCTTGGTAAGTAAGAAGGCTTTTCTGGATTTTTCAAATTACTGCGTTGAT ATTTCAATCGAGAAAGAAAATGGTACCGTCAGTGCTGGAAATAGAGCTTGGATCCAAGTAAAAATGCTGT GAAGAATGGTAAAGTCCCTCTGGGATAGCCTCTGCAACATGAGCCAAGACAGAAAACCCATCGTGGGGAG TTTCCACTTTGTTTGCGCCCTGGCCTTAATAGTCGGAAGCATGACCCCGTTCTCTAATGAACTTGAGTCT ATGGTAGACTATTCAAACAGGAACCTTACTCATGTCCCCAAAGACCTGCCACCAAGAACAAAAGCCCTGA GTCTGTCTCAAAACTCTATATCTGAGCTTCGGATGCCTGATATCAGCTTTCTGTCAGAGCTGAGAGTTCT GAGACTCTCCCACAACAGGATACGGAGCCTTGATTTCCATGTATTCTTGTTCAATCAGGACTTAGAATAC CTGGATGTCTCACACAATCGGTTGCAAAACATCTCTTGCTGCCCTATGGCGAGCCTGAGGCATCTAGACC TCTCATTCAATGACTTTGATGTACTGCCTGTGTGTAAGGAATTTGGCAACCTGACGAAGCTGACTTTCCT GGGATTAAGTGCTGCAAAGTTCCGACAACTGGATCTGCTCCCAGTTGCTCACTTGCATCTAAGCTGCATT CTTCTGGACTTAGTGAGTTATCATATAAAAGGCGGGGAAACAGAAAGTCTTCAGATTCCCAATACCACCG TTCTCCATTTGGTCTTTCATCCAAATAGCTTGTTCTCTGTTCAAGTGAACATGTCTGTAAACGCTTTAGG ACATTTACAACTGAGTAATATTAAATTGAATGATGAAAACTGTCAAAGGTTAATGACATTTTTATCAGAA CTCACCAGAGGTCCAACCTTATTGAATGTGACCCTCCAGCACATAGAAACAACCTGGAAGTGCTCGGTTA AACTTTTCCAATTCTTTTGGCCCCGACCGGTGGAGTACCTCAATATTTACAACTTAACGATAACTGAGAG AATCGACAGGGAAGAATTTACTTACTCGGAGACAGCACTGAAGTCACTGATGATAGAGCACGTCAAAAAC CAAGTGTTCCTCTTTTCAAAGGAGGCGCTATACTCGGTGTTTGCTGAGATGAACATCAAGATGCTCTCTA TCTCAGACACCCCTTTCATCCACATGGTGTGCCCGCCATCCCCAAGCTCATTTACATTTCTGAACTTTAC CCAGAATGTTTTTACTGACAGTGTTTTTCAAGGCTGTTCCACCTTAAAGAGATTGCAGACACTTATCTTA CAAAGGAATGGTTTGAAGAACTTTTTTAAAGTAGCTCTCATGACTAAGAATATGTCCTCTCTGGAAACTT TGGATGTTAGTTTGAATTCTTTGAACTCTCATGCATATGACAGGACATGCGCCTGGGCTGAGAGCATATT GGTGTTGAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCAGATGCTTACCTCCCAAGGTCAAGGTC CTTGACCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGTCACCCACCTGCAGGCTTTGCAGGAAC TCAATGTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGTGGGGCCTTCAGCAGCCTTTCTGTGCTGGT CATCGACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCCAGAGCTGTCAGAATATTAGATCCCTA ACAGCGGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGACTTTGTCAAGAACATAGGCTGGGTAG CAAGAGAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGACTACCCAGAAAGCTCTAAGGGAACTGC ACTGAGGGACTTCCACATGTCTCCACTGTCCTGTGATACTGTTCTGCTGACTATCACCATCGGGGCCACT ATGCTGGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTTTGACCTGCCCTGGTATGTGAGGATGC TGTGTCAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCCTTAGAGGAACTCCAGAGAAACCTCCA GTTCCATGCTTTTGTCTCATACAGTGAGCATGATTCTGCCTGGGTGAAGAACGAATTACTACCCAACCTA GAGAAAGATGACATCCGGGTTTGCCTCCATGAGAGGAACTTTGTCCCTGGCAAGAGCATTGTGGAGAACA TCATCAATTTCATTGAGAAGAGTTACAAGGCCATCTTTGTGCTGTCTCCCCACTTCATCCAGAGTGAGTG GTGCCATTATGAACTCTATTTTGCCCATCATAATCTCTTCCATGAAGGCTCTGATAACTTAATCCTCATC TTGCTGGAACCCATTCTACAGAACAACATTCCCAGTAGATACCACAAGCTGCGGGCTCTCATGGCACAGC GGACTTACTTGGAATGGCCTACTGAGAAGGGCAAACGTGGGCTGTTTTGGGCCAACCTTAGAGCTTCATT TATTATGAAGTTAGCCTTAGTCAATGAGGATGATGTGAAAACTTGAAACTTGGGTTTCTAACTTAATAAA CTGTCAACCTGGAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_011604
• Insert Size: 2421 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC055366, AAH55366
• RefSeq Size: 2625 bp
• RefSeq ORF: 2421 bp
• Locus ID: 21899
• UniProt ID: Q9EPW9
• Cytogenetics: 5 33.54 cM
• Gene Summary: Participates in the innate immune response to Gram-positive bacteria and fungi. Specifically recognizes diacylated and, to a lesser extent, triacylated lipopeptides (PubMed:19931471). In response to diacylated lipopeptides, forms the activation cluster TLR2:TLR6:CD14:CD36, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. In complex with TLR4, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion (PubMed:20037584, PubMed:23812099).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.