Ace2 (NM_001130513) Mouse Tagged ORF Clone

CAT#: MR210755

Ace2 (Myc-DDK-tagged) - Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 (Ace2), transcript variant 1

Product Images

Circular map for MR210755

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ace2
• Synonyms: 2010305L05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210755 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCTCCTCCTGGCTCCTTCTCAGCCTTGTTGCTGTTACTACTGCTCAGTCCCTCACCGAGGAAA
  ATGCCAAGACATTTTTAAACAACTTTAATCAGGAAGCTGAAGACCTGTCTTATCAAAGTTCACTTGCTTC
  TTGGAATTATAATACTAACATTACTGAAGAAAATGCCCAAAAGATGAGTGAGGCTGCAGCCAAATGGTCT
  GCCTTTTATGAAGAACAGTCTAAGACTGCCCAAAGTTTCTCACTACAAGAAATCCAGACTCCGATCATCA
  AGCGTCAACTACAGGCCCTTCAGCAAAGTGGGTCTTCAGCACTCTCAGCAGACAAGAACAAACAGTTGAA
  CACAATTCTGAACACCATGAGCACCATTTACAGTACTGGAAAAGTTTGCAACCCAAAGAACCCACAAGAA
  TGCTTATTACTTGAGCCAGGATTGGATGAAATAATGGCGACAAGCACAGACTACAACTCTAGGCTCTGGG
  CATGGGAGGGCTGGAGGGCTGAGGTTGGCAAGCAGCTGAGGCCGTTGTATGAAGAGTATGTGGTCCTGAA
  AAACGAGATGGCAAGAGCAAACAATTATAACGACTATGGGGATTATTGGAGAGGGGACTATGAAGCAGAG
  GGAGCAGATGGCTACAACTATAACCGTAACCAGTTGATTGAAGATGTAGAACGTACCTTCGCAGAGATCA
  AGCCATTGTATGAGCATCTTCATGCCTATGTGAGGAGGAAGTTGATGGATACCTACCCTTCCTACATCAG
  CCCCACTGGATGCCTCCCTGCCCATTTGCTTGGTGATATGTGGGGTAGATTTTGGACAAATCTGTACCCT
  TTGACTGTTCCCTTTGCACAGAAACCAAACATAGATGTTACTGATGCAATGATGAATCAGGGCTGGGATG
  CAGAAAGGATATTTCAAGAGGCAGAGAAATTCTTTGTTTCTGTTGGCCTTCCTCATATGACTCAAGGATT
  CTGGGCAAACTCTATGCTGACTGAGCCAGCAGATGGCCGGAAAGTTGTCTGCCACCCCACAGCTTGGGAT
  CTGGGACACGGAGACTTCAGAATCAAGATGTGTACAAAGGTCACAATGGACAACTTCTTGACAGCCCATC
  ACGAGATGGGACACATCCAATATGACATGGCATATGCCAGGCAACCTTTCCTGCTAAGAAACGGAGCCAA
  TGAAGGGTTCCATGAAGCTGTTGGAGAAATCATGTCACTTTCTGCAGCTACCCCCAAGCATCTGAAATCC
  ATTGGTCTTCTGCCATCCGATTTTCAAGAAGATAGCGAAACAGAGATAAACTTCCTACTGAAACAGGCAT
  TGACAATTGTTGGAACACTACCGTTTACTTACATGTTAGAGAAGTGGAGGTGGATGGTCTTTCGGGGTGA
  AATTCCCAAAGAGCAGTGGATGAAAAAGTGGTGGGAGATGAAGCGGGAGATCGTTGGTGTGGTGGAGCCT
  CTGCCTCATGATGAAACATACTGTGACCCTGCATCTCTGTTCCATGTTTCTAATGATTACTCATTCATTC
  GATATTACACAAGGACCATTTACCAATTCCAGTTTCAAGAAGCTCTTTGTCAAGCAGCTAAGTATAATGG
  TTCTCTGCACAAATGTGACATCTCAAATTCCACTGAAGCTGGGCAGAAGTTGCTCAAGATGCTGAGTCTT
  GGAAATTCAGAGCCCTGGACCAAAGCCTTGGAAAATGTGGTAGGAGCAAGGAATATGGATGTAAAACCAC
  TGCTCAATTACTTCCAACCGTTGTTTGACTGGCTGAAAGAGCAGAACAGAAATTCTTTTGTGGGGTGGAA
  CACTGAATGGAGCCCATATGCCGACCAAAGCATTAAAGTGAGGATAAGCCTAAAATCAGCTCTTGGAGCT
  AATGCATATGAATGGACCAACAACGAAATGTTCCTGTTCCGATCATCTGTTGCATATGCCATGAGAAAGT
  ATTTTTCAATAATCAAAAACCAGACAGTTCCTTTTCTAGAGGAAGATGTACGAGTGAGTGATTTGAAACC
  AAGAGTCTCCTTCTACTTCTTTGTCACCTCACCCCAAAATGTGTCTGATGTCATTCCTAGAAGTGAAGTT
  GAAGATGCCATCAGGATGTCTCGGGGCCGCATCAATGATGTCTTTGGCCTGAATGATAACAGCCTGGAGT
  TTCTGGGGATTCACCCAACACTTGAGCCACCTTACCAGCCTCCTGTCACCATATGGCTGATTATTTTTGG
  TGTTGTGATGGCACTGGTAGTGGTTGGCATCATCATCCTGATTGTCACTGGCATCAAAGGTCGAAAGAAG
  AAAAATGAAACAAAAAGAGAAGAGAACCCTTATGACTCGATGGACATTGGAAAAGGAGAAAGCAATGCAG
  GATTCCAAAACAGTGATGATGCTCAGACTTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210755 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSSSWLLLSLVAVTTAQSLTEENAKTFLNNFNQEAEDLSYQSSLASWNYNTNITEENAQKMSEAAAKWS
  AFYEEQSKTAQSFSLQEIQTPIIKRQLQALQQSGSSALSADKNKQLNTILNTMSTIYSTGKVCNPKNPQE
  CLLLEPGLDEIMATSTDYNSRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYNDYGDYWRGDYEAE
  GADGYNYNRNQLIEDVERTFAEIKPLYEHLHAYVRRKLMDTYPSYISPTGCLPAHLLGDMWGRFWTNLYP
  LTVPFAQKPNIDVTDAMMNQGWDAERIFQEAEKFFVSVGLPHMTQGFWANSMLTEPADGRKVVCHPTAWD
  LGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYARQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKS
  IGLLPSDFQEDSETEINFLLKQALTIVGTLPFTYMLEKWRWMVFRGEIPKEQWMKKWWEMKREIVGVVEP
  LPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKYNGSLHKCDISNSTEAGQKLLKMLSL
  GNSEPWTKALENVVGARNMDVKPLLNYFQPLFDWLKEQNRNSFVGWNTEWSPYADQSIKVRISLKSALGA
  NAYEWTNNEMFLFRSSVAYAMRKYFSIIKNQTVPFLEEDVRVSDLKPRVSFYFFVTSPQNVSDVIPRSEV
  EDAIRMSRGRINDVFGLNDNSLEFLGIHPTLEPPYQPPVTIWLIIFGVVMALVVVGIIILIVTGIKGRKK
  KNETKREENPYDSMDIGKGESNAGFQNSDDAQTSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130513
• ORF Size: 2418 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130513.1, NP_001123985.1
• RefSeq Size: 3566 bp
• RefSeq ORF: 2418 bp
• Locus ID: 70008
• UniProt ID: Q8R0I0
• Cytogenetics: X 76.12 cM
• MW: 92.4 kDa
• Gene Summary: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. May have a protective role in acute lung injury. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19, regulating its trafficking on the cell surface and its activity (PubMed:19185582).[UniProtKB/Swiss-Prot Function]