Kifc3 (NM_001145832) Mouse Tagged ORF Clone

CAT#: MR210694

Kifc3 (Myc-DDK-tagged) - Mouse kinesin family member C3 (Kifc3), transcript variant 3

Product Images

Circular map for MR210694

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kifc3
• Synonyms: AI325457; BB123200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210694 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGCCCCGCCCCAGCCAGCCCAGCCGCCCGCCCTTTCCCACACACCGGCCAGGGGAGGTTGAGAA
  CTGGGCGTGGAAAAGATATTCTACCCAGTGGCGAGGAAGACTCCACTTCTCGGACTGCAGCTCGCCCGTC
  TCTTGCACAGTGTCGAGCCCTCAGCGTGGACTGGCCTGGCCCTAGGAGTCCCCATAGACTCTACCTGACA
  GTGCAGGTGGAGAATCTAAAGGAAAAGCTCATTAGCCAGGCCCAGGAAGTGAGCCGACTGCGATCAGAAC
  TGGGAGGCACGGACGCGGAGAAGCACAGGGACAGACTGATGGTGGAGAATGAGCAGCTGAGGCAGGAGCT
  GAGGCGCTGTGAGGTCGAACTGCAGGAGCTTCGGGCCCAGCCGGTGGTGCCCTGCGAGGGCTGTGAGCAC
  AGCCAGGAGAGCAGCCAGCTCCGTGACAAGCTGTCCCAGCTGCAGTTGGAAGTGGCTGAGAACAAAGGCA
  TGCTGTCGGAACTGAACCTGGAGGTGCAGCAGAAAACGGACCGGCTGGCAGAGGTAGAGCTGCGCCTCAA
  GGACTGTCTGGCTGAGAAAGCCCAGGAGGAGGAACGGCTCAGCCGGCGCCTGCGTGACAGCCATGAGACT
  ATCGCCAGTCTGAGGGCCCAGTCCCCACCTGTTAAGTATGTCATCAAGACAGTGGAGGTAGAGTCTTCCA
  AGACCAAGCAGGCCCTCAGCGAGTCCCAGACCCGGAACCAGCACCTGCAGGAGCAAGTGGCCATGCAGCG
  GCAGGTGCTGAAGGAGATGGAGCAGCAGCTACAGAACTCCCACCAGCTGACAGTCCAGTTGCGGGCTCAG
  ATTGCCATGTATGAGGCAGAGCTGGAGCGGGCACACGGGCAGATGCTGGAGGAGATGCAGTCCTTGGAGG
  AGGATAAGAACCGGGCCATTGAGGAGGCCTTTGCACGGGCCCAGGTGGAGATGAAGGCAGTACACGAGAA
  CCTGGCAGGTGTCAGGACCAACCTGCTGACGCTGCAGCCTGCGCTGAGGACCCTCACCAACGACTACAAT
  GGGCTCAAGCGGCAGGTGCGGGGCTTCCCCTTGCTGCTGCAGGAGGCTCTCAGGAGTGTCAAAGCTGAGA
  TCGGCCAGGCCATCGAGGAGGTTAATAGCAACAACCAGGAGCTGTTGCGCAAGTACCGCCGGGAACTACA
  GCTGCGCAAGAAATGCCATAACGAGCTGGTCCGGCTGAAGGGAAACATCCGGGTGATTGCCCGAGTCCGG
  CCAGTCACCAAGGAGGATGGAGAAGGACCCGAGGCAACCAATGCTGTGACCTTTGACCCTGATGATGACT
  CCATCATCCACCTGCTGCACAAGGGCAAGCCCGTGTCCTTCGAGCTGGACAAGGTCTTCTCCCCGTGGGC
  TTCCCAGCAGGACGTGTTCCAGGAGGTGCAGGCCCTCATTACCTCCTGCATCGATGGCTTCAATGTCTGT
  ATCTTTGCTTACGGCCAGACAGGTGCCGGCAAGACATATACAATGGAGGGGACTCCCGAGAACCCGGGCA
  TCAACCAGCGGGCCCTGCAGCTGCTCTTCTCGGAGGTGCAGGAGAAGGCGTCTGACTGGCAGTACAACAT
  CACCGTCAGCGCAGCTGAGATTTACAATGAAGTCCTCAGGGACCTGCTGGGGAAAGAGCCTCAGGAGAAG
  CTGGAGATCCGACTGTGCCCGGATGGCAGTGGGCAACTGTATGTGCCAGGGCTGACTGAGTTCCAGGTGC
  AGAGCGTGGATGACATCAACAAGGTGTTTGAGTTTGGCTACAACAACCGCACCACCGAGTTCACCAACCT
  GAACGAACACAGCTCCCGCTCCCACGCTCTGCTCATCGTGACAGTGAGAGGTGTGGATTGCAGCACAGGC
  CTCCGCACCACGGGGAAGCTGAACCTGGTGGACCTGGCTGGTTCAGAGCGTGTGGGCAAGTCGGGGGCTG
  AGGGCAACCGCCTGCGAGAGGCACAGCACATCAACAGGTCACTATCCGCCCTGGGGGACGTCATTGCTGC
  CCTGCGTTCTCGACAAGGCCATGTGCCCTTCCGCAATTCCAAACTCACCTACCTGCTGCAGGACTCACTC
  AGTGGTGACAGCAAGACCCTTATGGTGGTACAGGTGTCCCCAGTGGAGAAGAACACCAGCGAGACTCTGT
  ACTCCCTCAGATTTGCTGAGAGGGTTCGCTCTGTGGAGTTGGGCCCTGGGTCCCGCCGCACAGAGCTGGG
  TTCCTGGTCAAGCCAGGAGCACCTGGAGTGGGAGCCGGCTTGCCAGACACCACAGCCTACAGCACGAGCC
  CACTCTGCCCCTGGCTCTGGGACCAGTAGCCGCCCAGGTTCCATCCGAAGGAAACTGCAGCCGTCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210694 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARPAPASPAARPFPHTGQGRLRTGRGKDILPSGEEDSTSRTAARPSLAQCRALSVDWPGPRSPHRLYLT
  VQVENLKEKLISQAQEVSRLRSELGGTDAEKHRDRLMVENEQLRQELRRCEVELQELRAQPVVPCEGCEH
  SQESSQLRDKLSQLQLEVAENKGMLSELNLEVQQKTDRLAEVELRLKDCLAEKAQEEERLSRRLRDSHET
  IASLRAQSPPVKYVIKTVEVESSKTKQALSESQTRNQHLQEQVAMQRQVLKEMEQQLQNSHQLTVQLRAQ
  IAMYEAELERAHGQMLEEMQSLEEDKNRAIEEAFARAQVEMKAVHENLAGVRTNLLTLQPALRTLTNDYN
  GLKRQVRGFPLLLQEALRSVKAEIGQAIEEVNSNNQELLRKYRRELQLRKKCHNELVRLKGNIRVIARVR
  PVTKEDGEGPEATNAVTFDPDDDSIIHLLHKGKPVSFELDKVFSPWASQQDVFQEVQALITSCIDGFNVC
  IFAYGQTGAGKTYTMEGTPENPGINQRALQLLFSEVQEKASDWQYNITVSAAEIYNEVLRDLLGKEPQEK
  LEIRLCPDGSGQLYVPGLTEFQVQSVDDINKVFEFGYNNRTTEFTNLNEHSSRSHALLIVTVRGVDCSTG
  LRTTGKLNLVDLAGSERVGKSGAEGNRLREAQHINRSLSALGDVIAALRSRQGHVPFRNSKLTYLLQDSL
  SGDSKTLMVVQVSPVEKNTSETLYSLRFAERVRSVELGPGSRRTELGSWSSQEHLEWEPACQTPQPTARA
  HSAPGSGTSSRPGSIRRKLQPSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145832
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145832.1, NP_001139304.1
• RefSeq Size: 2945 bp
• RefSeq ORF: 2064 bp
• Locus ID: 16582
• UniProt ID: O35231
• Cytogenetics: 8 47.12 cM
• MW: 89.1 kDa
• Gene Summary: Minus-end microtubule-dependent motor protein. Involved in apically targeted transport. Required for zonula adherens maintenance.[UniProtKB/Swiss-Prot Function]