Hid1 (NM_175454) Mouse Tagged ORF Clone

CAT#: MR210663

Hid1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C630004H02 gene (C630004H02Rik)

Product Images

Circular map for MR210663

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hid1
• Synonyms: C630004H02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210663 representing NM_175454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCCGCAGACTCCAAGCTGAACTTCCGGAAGGCGGTGATCCAGCTGACCACCAAGACGCAGCCCG
  TGGAAGCCACCGACAATGCTTTCTGGGACCAGTTCTGGGCAGACACCGCTACCTCGGTGCAGGATGTCTT
  TGCGTTGGTGCCGGCAGCAGAGATCCGGGCTGTGCGAGAGGAGTCACCCTCTAACCTGGCCACGCTGTGC
  TACAAGGCTGTGGAGAAGCTGGTGCAAGGAGCAGAGGGTGGCTGCCACTCTGAGAAGGAGAAACAGGTCG
  TCCTGAACTGCAGCCGGCTACTCACCCGAGTGCTGCCTTACATCTTCGAGGACCCCGACTGGAGGGGCTT
  CTTCTGGTCCACAGTGCCCGGGGCAGGGCGTGGAGGGCAGGGTGAGGAAGAGGATGAGAACGCTCGTCCC
  TTGGCTGAGTCCTTGCTCCTGGCCATTGCCGATTTGCTCTTCTGCCCAGACTTCACTGTGCAGAACCACC
  GGAGGAACGACGTGGACTCAGCAGAAGATGTCCATTCCCTGGACAGCTGTGAATATATCTGGGAGGCTGG
  TGTGGGCTTTGCTCATTCCCCACAGCCCAACTACATCCATGACATGAACCGGATGGAGCTGCTGAAGTTA
  CTGCTCACGTGCTTCTCAGAGGCCATGTACCTGCCCCCATCTCCAGAGAGTGGCAGCACCAACCCCTGGG
  TTCAGTTCTTTTGTTCCACGGAGAACAGACACGCGCTGCCCCTCTTCACTTCACTCCTCAACACCGTGTG
  TGCCTATGACCCTGTGGGCTACGGGATCCCCTACAACCACCTGCTCTTCTCGGACTACCGGGAGCCCCTG
  GTGGAGGAGGCTGCTCAGGTGCTTATTGTCACCTTGGACCATGACAGTGCTACCAGCACCAGCCCCACCG
  TGGACGGCACCACCACGGGCACAGCTATGGATGATGCTGATCCTCCAGGGCCCGAGAACCTGTTTGTGAA
  CTACCTGTCCCGAATTCATCGTGAGGAGGACTTCCAGTTCATCCTCAAGGGGATCGCTCGGCTGCTGTCC
  AATCCCCTGCTCCAGACCTACCTTCCCAACTCCACCAAGAAGATCCAGTTTCACCAGGAGTTGCTTGTTC
  TCTTCTGGAAACTCTGTGACTTCAACAAGAAATTCCTCTTCTTTGTACTGAAGAGCAGTGACGTGCTGGA
  TATCCTGGTTCCTATCCTCTATTTTCTCAACGACGCCCGGGCAGATCAATCTCGGGTGGGCTTGATGCAC
  ATCGGGGTCTTCATCCTGCTGCTCCTGAGTGGGGAGAGGAACTTTGGGGTACGGCTGAACAAGCCCTACT
  CAGTGCGCGTGCCCATGGACATCCCGGTCTTCACAGGCACACATGCAGATTTGCTCATTGTGGTATTCCA
  CAAAATCATCACCAGCGGTCATCAGCGGTTGCAACCCCTCTTCGACTGCCTGCTCACCATTGTGGTCAAC
  GTGTCTCCCTACCTCAAGAGCCTGTCCATGGTGACTGCCAACAAGCTCCTGCATCTGCTCGAGGCCTTCT
  CGACCACCTGGTTCCTCTTCTCGGCATCTCAGAACCACCATCTGGTCTTCTTCCTTCTGGAGGTCTTCAA
  CAACATTATCCAGTACCAGTTTGATGGCAATTCCAACCTGGTCTATGCCATCATCCGTAAACGTGCGGTC
  TTCCACCAGCTGGCCAACCTGCCCACTGACCCACCCTCCATCCACAAGGCACTTCAGCGGCGGCGGAGGA
  CACCAGAGCCCCTGTCCCGTACTGGTTCCCAGGAGGGAACGTCCATGGAGGGATCCCGCCCTGCTGCCCC
  TGCAGAACCAGGCACCCTTAAGACTAGCCTGGTGGCCACTCCAGGCATCGACAAGCTGACAGAGAAATCC
  CAGGTATCAGAGGACGGCACCTTGAGGTCTCTAGAGCCCGAATCCCAGCAAAGCTCAGCAGAAAACAGCC
  CATCTGATGGGGAGTCCAGCCAAACATGGAGAGAGCAACGGAGATTGTCCAATGCATCAGCCAGTGGCCA
  GTGGAGCCCAACATCAGATTGGATCTTGTCCTGGAAGTCAAAGCTGCCGCTGCAGACCATCATGCGCCTG
  CTGCAGGTCCTGGTTCCCCAGGTGGAGAAGATCTGCATTGACAAGGGTCTGACGGATGAGTCTGAGATCC
  TGAGATTCCTGCAGCACGGTACCCTAGTGGGGCTTCTGCCTGTGCCCCACCCCATCCTTATCCGAAAGTA
  CCAGGCGAACTCGGGGACTGCCATGTGGTTCCGTACCTACATGTGGGGCGTTATCTATTTGAGGAATGTG
  GACCCACCTATCTGGTATGACACTGACGTGAAGCTGTTTGAGATCCAGCGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210663 representing NM_175454
  Red=Cloning site Green=Tags(s)
  
  MGSADSKLNFRKAVIQLTTKTQPVEATDNAFWDQFWADTATSVQDVFALVPAAEIRAVREESPSNLATLC
  YKAVEKLVQGAEGGCHSEKEKQVVLNCSRLLTRVLPYIFEDPDWRGFFWSTVPGAGRGGQGEEEDENARP
  LAESLLLAIADLLFCPDFTVQNHRRNDVDSAEDVHSLDSCEYIWEAGVGFAHSPQPNYIHDMNRMELLKL
  LLTCFSEAMYLPPSPESGSTNPWVQFFCSTENRHALPLFTSLLNTVCAYDPVGYGIPYNHLLFSDYREPL
  VEEAAQVLIVTLDHDSATSTSPTVDGTTTGTAMDDADPPGPENLFVNYLSRIHREEDFQFILKGIARLLS
  NPLLQTYLPNSTKKIQFHQELLVLFWKLCDFNKKFLFFVLKSSDVLDILVPILYFLNDARADQSRVGLMH
  IGVFILLLLSGERNFGVRLNKPYSVRVPMDIPVFTGTHADLLIVVFHKIITSGHQRLQPLFDCLLTIVVN
  VSPYLKSLSMVTANKLLHLLEAFSTTWFLFSASQNHHLVFFLLEVFNNIIQYQFDGNSNLVYAIIRKRAV
  FHQLANLPTDPPSIHKALQRRRRTPEPLSRTGSQEGTSMEGSRPAAPAEPGTLKTSLVATPGIDKLTEKS
  QVSEDGTLRSLEPESQQSSAENSPSDGESSQTWREQRRLSNASASGQWSPTSDWILSWKSKLPLQTIMRL
  LQVLVPQVEKICIDKGLTDESEILRFLQHGTLVGLLPVPHPILIRKYQANSGTAMWFRTYMWGVIYLRNV
  DPPIWYDTDVKLFEIQRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175454
• ORF Size: 2364 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175454.2, NP_780663.1
• RefSeq Size: 3237 bp
• RefSeq ORF: 2367 bp
• Locus ID: 217310
• UniProt ID: Q8R1F6
• Cytogenetics: 11 E2
• MW: 89.2 kDa