Pfkp (NM_019703) Mouse Tagged ORF Clone

CAT#: MR210641

Pfkp (Myc-DDK-tagged) - Mouse phosphofructokinase, platelet (Pfkp)

Product Images

Circular map for MR210641

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pfkp
• Synonyms: 1200015H23Rik; 9330125N24Rik; ATP-PFK; PFK-C; PFK-P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210641 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACTTGGATTCTTCGAGCTCCAGCGCCTATCCGAAGTACCTGGAGCACCTCTCTGGGGATGGCA
  AAGCTATCGGTGTCCTGACCAGCGGCGGGGATGCCCAAGGCATGAATGCTGCTGTCCGCGCTGTGGTGCG
  CATGGGAATCTACACGGGGGCCAAAGTGTACTTTATATATGAGGGTTACCAAGGCTTGGTGGATGGAGGC
  TCCAATATTGTGGAAGCCAAATGGGACTGTGTCTCCAGCATTCTGCAAGTGGGTGGGACCATCATCGGTA
  GTGCGCGCTGCAAAGCCTTTCGAAGCCGTGAAGGGCGCCTGAAAGCTGCCTGTAACTTGGCGCGCTTGGG
  CATAACCAATCTGTGCGTGATCGGCGGGGACGGAAGTCTCACGGGAGCCAACCTCTTCCGAAAGGAGTGG
  AGCGGACTTCTGGAAGAGCTGGCTCGGAATGGTGATATCGATAACGACACAGTGCAGAAGTATTCCTACC
  TCAACGTGGTGGGTATGGTGGGCTCCATTGACAATGACTTCTGTGGCACAGACATGACCATTGGTACAGA
  TTCAGCCCTGCACCGAATTATTGAAGTTGTTGATGCCATCATGACCACTGCTCAGAGCCACCAGAGGACC
  TTCGTCCTGGAGGTGATGGGGAGACACTGTGGTTACTTGGCCTTGGTGAGCGCCTTGACTTGCGGTGCCG
  ACTGGGTGTTCCTTCCGGAGTCTCCACCTGAGGAAGATTGGGAGGAAAATATGTGCCTCAAACTCTCAGA
  GAACCGAGCCCGAAAAAAGAGGCTGAATATCATCATTGTGTCTGAAGGAGCAATCGACATGCAAAATAAG
  CCAATCACCTCTGAGAAAATCAAGGAGCTTGTAGTGAAGAATTTGGGATTTGACACCCGGGTCACCATCC
  TTGGGCATGTCCAACGAGGAGGGACCCCATCTGCATTTGACAGGATTTTGGCCAGCCGTATGGGAGTGGA
  GGCTGTCATTGCCTTGCTGGAAGCTACCCCCGAGACCCCAGCCTGTGTCGTGTCACTAAGAGGAAACCAA
  GCAGTGCGCCTGCCACTGATGGAGTGTGTGCAAATGACCCAGGATGTACAGAAGGCAATGGATGAAAGAA
  GATTTAAGGAAGCCGTGAAACTCCGAGGAAGGCGTTTTGAGGGAAACCTGAACACCTATAAGCGTCTTGC
  CATTAAACTGCCTGATGAGAAGATTGTCAAGAGCAACTGCAATGTAGCCGTCATCAATGTAGGGGCACCT
  GCTGCCGGAATGAATGCAGCCGTGCGCTCTGCTGTTCGAGTCGGGATTGCAGACGGCCACAAGATGTTTG
  CAATCTATGACGGCTTTGAAGGATTCGCCAATGGCCAAATCAAAGAAATCGGCTGGGCAGATGTCGGAGG
  CTGGACTGGACAAGGAGGGTCCATTCTTGGGACAAAACGCACCCTACCTGGAAAGTACTTGGAGAAGATC
  GCAGAGCAGATGCATTCCCATAGTATCAATGCCCTTCTGATCATTGGAGGATTTGAGGCCTACCTAGGAC
  TCCTAGAGCTGGCAGCTGCCCGGGAGAAACATGAGGCGTTCTGTGTCCCCATGGTTATGGTTCCTGCTAC
  TGTCTCCAACAATGTGCCGGGTTCCGATTTCAGCATCGGGGCAGACACAGCTCTGAACACTATCACAGAC
  ACGTGCGACCGCATTAAACAGTCAGCCAGTGGGACCAAGCGCCGGGTGTTCATCATTGAGACCATGGGTG
  GATACTGTGGCTACCTGGCCAACATGGGGGCCCTTGCAGCAGGAGCTGATGCTGCCTACATCTTTGAAGA
  GCCATTTGATATCGGAGATTTGCAGTCCAACGTCGTGCACTTGACAGAGAAAATGAAGACCAGCATCCAG
  AGGGGCCTCGTACTCAGAAACGAAAGCTGCAGTGTAAATTATACCACTGACTTCATCTACCAGCTCTACT
  CAGAGGAGGGCAAAGGAGTGTTTGACTGCAGGAAAAACGTGCTGGGCCATATGCAGCAGGGGGGAGCACC
  GTCTCCATTCGATAGGAACTTTGGAACCAAGATTTCTGCCAAAGCAATGGAGTGGATCTCGGCCAAACTG
  AAGGGGTCCCAGGGCACAGGGAAAAAATTTGTTAGCGATGATTCCATTTGTGTCCTGGGAATTTGCAAGA
  GAGATCTCCTGTTTCAACCAGTGGCAGAGCTGAAGAAAGTCACTGACTTTGAGCACAGGATCCCCAAGGA
  GCAGTGGTGGCTGAAACTGCGGCCCATCATGAAGATCTTGGCCAAGTATGAGGCGAGCTATGACATGTCT
  GATTCAGGCAAGCTGGAGTCCTTGCAGCACCATGAAGAACTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210641 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDLDSSSSSAYPKYLEHLSGDGKAIGVLTSGGDAQGMNAAVRAVVRMGIYTGAKVYFIYEGYQGLVDGG
  SNIVEAKWDCVSSILQVGGTIIGSARCKAFRSREGRLKAACNLARLGITNLCVIGGDGSLTGANLFRKEW
  SGLLEELARNGDIDNDTVQKYSYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRT
  FVLEVMGRHCGYLALVSALTCGADWVFLPESPPEEDWEENMCLKLSENRARKKRLNIIIVSEGAIDMQNK
  PITSEKIKELVVKNLGFDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPETPACVVSLRGNQ
  AVRLPLMECVQMTQDVQKAMDERRFKEAVKLRGRRFEGNLNTYKRLAIKLPDEKIVKSNCNVAVINVGAP
  AAGMNAAVRSAVRVGIADGHKMFAIYDGFEGFANGQIKEIGWADVGGWTGQGGSILGTKRTLPGKYLEKI
  AEQMHSHSINALLIIGGFEAYLGLLELAAAREKHEAFCVPMVMVPATVSNNVPGSDFSIGADTALNTITD
  TCDRIKQSASGTKRRVFIIETMGGYCGYLANMGALAAGADAAYIFEEPFDIGDLQSNVVHLTEKMKTSIQ
  RGLVLRNESCSVNYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISAKAMEWISAKL
  KGSQGTGKKFVSDDSICVLGICKRDLLFQPVAELKKVTDFEHRIPKEQWWLKLRPIMKILAKYEASYDMS
  DSGKLESLQHHEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019703
• ORF Size: 2355 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019703.4, NP_062677.1
• RefSeq Size: 4033 bp
• RefSeq ORF: 2355 bp
• Locus ID: 56421
• UniProt ID: Q9WUA3
• Cytogenetics: 13 A1
• MW: 85.5 kDa
• Gene Summary: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function]