Slc7a14 (NM_172861) Mouse Tagged ORF Clone

CAT#: MR210563

Slc7a14 (Myc-DDK-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 (Slc7a14)

Product Images

Circular map for MR210563

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc7a14
• Synonyms: A930013N06; BC061928
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210563 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCTTTCTGGCCTCACTGGACCCGAGGCGGGTACAGTGGGGAGCCGCCTGGTATGCAATGCACT
  CTCGGATCCTGCGCACCAAACCAGTGGAGTCCATGCTGGAGGGAACTGGGACCACCTCGGCGCATGGTAC
  CAAGCTAGCCCAGGTTCTCACCACTGTAGACCTCATCTCTCTTGGAGTCGGCAGCTGTGTGGGCACTGGC
  ATGTATGTGGTGTCTGGCCTGGTGGCCAAGGAGATGGCAGGGCCTGGGGTCATCGTGTCCTTCATCATTG
  CTGCTGTTGCATCTATATTATCAGGTGTGTGCTATGCAGAGTTTGGAGTCCGAGTCCCCAAGACCACAGG
  ATCTGCCTACACCTACAGCTATGTCACCGTTGGGGAGTTCGTGGCATTTTTCATCGGCTGGAACTTGATT
  CTGGAGTACCTGATTGGCACGGCAGCTGGTGCCAGTGCTCTGAGCAGTATGTTTGACTCCCTGGCAAACC
  ACAGCATCAGCCGCTGGATGGTGGACACCGTGGGAACCCTCAACGGCCTGGGGAAAGGGGAAGAGTCGTA
  CCCAGACCTTCTGGCTCTGGTGATTGCCGTCATCGTGACCATCATTGTGGCTCTGGGAGTGAAAAACTCT
  GTGGGCTTCAACAACGTCCTCAATGTGCTGAACCTGGCAGTGTGGGTGTTCATCATGATCGCAGGCCTCT
  TCTTCATCAATGGCAAATACTGGGCTGAAGGCCAGTTCTTGCCCCACGGATGGTCAGGGGTGCTGCAAGG
  AGCAGCCACGTGCTTCTATGCTTTCATTGGCTTTGACATCATTGCCACCACGGGTGAGGAAGCCAAGAAC
  CCCAACACATCCATCCCTTACGCCATCACGGCATCCCTGGTCATCTGCCTGACAGCGTATGTGTCTGTGA
  GCATGATCTTAACCCTGATGGTACCATATTACGCCATTGACACAGAGTCCCCGCTCATGGAGATGTTTGT
  GGCCCATGGCTTCTATGCTGCGAAGTTTGTAGTGGCCATTGGCTCGGTGGCAGGACTGACCGTCAGCTTG
  CTGGGCTCCCTGTTCCCCATGCCCAGGGTCATTTACGCCATGGCTGGCGATGGGCTGCTTTTCAGGTTCC
  TGGCTCACGTGAGCTCCTACACAGAGACACCAGTGGTAGCTTGCATAGTGTCAGGCTTCCTGGCTGCTCT
  CCTCTCTCTGCTGGTGAGCCTGAGGGACCTCATAGAGATGATGTCCATCGGCACGCTCCTCGCCTACACC
  CTGGTCTCTGTCTGTGTCTTGCTTCTTCGGTATCAACCTGAGAGCGACATCGATGGTTTTGTCAAGTTCC
  TGTCTGAGGAGCACACCAAGAAGAAGGAAGGCATCCTGGCTGACTGTGAGAAGGAAACTTGTTCTCCTGT
  GAGTGAAGGGGAGGAATTTTCCAGCCCAGCCACCAACACCTGTGGGGCCAAAAACCTGCCGTCCTTGGGA
  GACAATGAGATGCTCATAGGGAAATCAGATAAGTCCGCCTACAACGTCAACCACCCCAACTACGGCACTG
  TGGATATGACCACGGGCATAGAAGCCGATGAATCAGAAAACATCTATCTCATCAAGTTGAAGAAGCTGAT
  CGGACCCCGTTACTACACCATGAGAATCCGGCTGGGCCTTCCAGGCAAAATGGACCGGCCCACGGCGGCG
  ACCGGGCACACGGTGACCATCTGCGTGCTCCTGCTGTTCATCCTCATGTTCATCTTCTGCTCCTTCATCA
  TCTTTGGCTCTGAGTACATCTCAGGCCAGAGCTGGTGGGCCATCCTTCTGGTCGTTCTGATGATGCTGCT
  GATCAGTGTGCTGGTGTTTGTAATCCTGCAGCAGCCAGAGAACCCCAAGAAGCTGCCGTACATGGCCCCC
  TGCCTCCCCTTTGTACCTGCCTTTGCCATGTTGGTGAATATCTATCTCATGCTAAAGCTCTCCACCATCA
  CATGGATCCGGTTTGCGGTCTGGTGCTTTGTGGGTATGCTCATTTATTTTGGGTATGGCATCTGGAACAG
  CACCTTGGAAATCAGCGCCCGAGAGCAAGCCCTGCATCAGAGCACGTACCAGCGCTACGATGTGGATGAT
  CCCTTTTCTGTGGAGGAGGGGTTCTCCTACGCCACCGAGGGCGAGAGCCAGGAGGACTGGGGTGGGCCCG
  CCGAGGACAAAGGCTTCTATTACCAACAGATGTCAGATGCGAAGGCAAACAGCCGGACGAGTAGCAAAGC
  GAAAAGCAAAAGCAAACACAAACAGAACTCGGAGGCCCTGATTGCAAATGACGAGTTAGATTGCTCTCCG
  GAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210563 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGFLASLDPRRVQWGAAWYAMHSRILRTKPVESMLEGTGTTSAHGTKLAQVLTTVDLISLGVGSCVGTG
  MYVVSGLVAKEMAGPGVIVSFIIAAVASILSGVCYAEFGVRVPKTTGSAYTYSYVTVGEFVAFFIGWNLI
  LEYLIGTAAGASALSSMFDSLANHSISRWMVDTVGTLNGLGKGEESYPDLLALVIAVIVTIIVALGVKNS
  VGFNNVLNVLNLAVWVFIMIAGLFFINGKYWAEGQFLPHGWSGVLQGAATCFYAFIGFDIIATTGEEAKN
  PNTSIPYAITASLVICLTAYVSVSMILTLMVPYYAIDTESPLMEMFVAHGFYAAKFVVAIGSVAGLTVSL
  LGSLFPMPRVIYAMAGDGLLFRFLAHVSSYTETPVVACIVSGFLAALLSLLVSLRDLIEMMSIGTLLAYT
  LVSVCVLLLRYQPESDIDGFVKFLSEEHTKKKEGILADCEKETCSPVSEGEEFSSPATNTCGAKNLPSLG
  DNEMLIGKSDKSAYNVNHPNYGTVDMTTGIEADESENIYLIKLKKLIGPRYYTMRIRLGLPGKMDRPTAA
  TGHTVTICVLLLFILMFIFCSFIIFGSEYISGQSWWAILLVVLMMLLISVLVFVILQQPENPKKLPYMAP
  CLPFVPAFAMLVNIYLMLKLSTITWIRFAVWCFVGMLIYFGYGIWNSTLEISAREQALHQSTYQRYDVDD
  PFSVEEGFSYATEGESQEDWGGPAEDKGFYYQQMSDAKANSRTSSKAKSKSKHKQNSEALIANDELDCSP
  E
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172861
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172861.3, NP_766449.1
• RefSeq Size: 8869 bp
• RefSeq ORF: 2316 bp
• Locus ID: 241919
• UniProt ID: Q8BXR1
• Cytogenetics: 3 A3
• MW: 84 kDa
• Gene Summary: May be involved in arginine transport.[UniProtKB/Swiss-Prot Function]