Spire2 (NM_172287) Mouse Tagged ORF Clone

CAT#: MR210260

Spire2 (Myc-DDK-tagged) - Mouse spire homolog 2 (Drosophila) (Spire2)

Product Images

Circular map for MR210260

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spire2
• Synonyms: BC026502; Spir-2; Spir2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210260 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGGGCGGGCGGCGGCGGCGCAGCAGCTCCCGAACGCGCGGGGGGCGCGGCGCGGCCCGAGCCGT
  GGGAGCTGTCCCTGGAGGAAGTGCTGAAGGTGTACGAGCAGCCCATCAACGAGGAGCAGGCCTGGGCGGT
  GTGCTTCCAGGGCTGCCGCGGGCTGCGGGGCGAGCCGGGCGGCGTGCGGCGCATCCGGGACACGGCAGAC
  ATCCTCCTGCGTAGGGACGGTTCGGTTGGCGCGCGGCTGGAGCCCGAGCCTACAACCATGGTGGTACCAC
  CAGCCAGCTCAGAAGCCCAGATGGTGCAGTCACTGGGCTTTGCCATTTACCGTGCGCTGGACTGGGGGTT
  AGATGAGAACGAGGAGAGAGAGCTCAGCCCGCAGCTGGAAAGGCTCATCGACCTCATGGCCAACAGCGAC
  TGCGAAGACAGCAGCTGCGGGGCAGCGGATGAGGGCTACGTGGGTCCAGAAGAGGAGGAGGAGGCTGAGG
  GCGGCCCCCGAGCTGTGCGCACCTTTGCCCAGGCTATGCGGCTGTGTGCTTTGCGCCTGACCGACCCCCA
  CGGCGCGCAGGCCCACTACCAGGCAGTGTGCCGAGCTCTCTTTGTGGAGACACTGGAGCTTCGTGCCTTC
  CTGGCCCGGGTGAGGGAGGCCAAAGAGATGCTGAAGAAACTGGGGGAGGAGGAGCCACGGGAGAAGCCAC
  TGGCCGAGCTCGACCACCTGGGACACACAGACTGGGCCCGGCTGTGGGTCCAGCTCATGCGGGAGCTCCG
  TCACGGGGTAAAGCTGAAGAAGGTGCAGGAGAAGGAGTTCAACCCACTGCCCACCGAGTTCCAGCTCACC
  CCCTTCGAGATGCTGATGCAGGATATCCGTGCCAGGAACTATAAGCTGCGCAAGGTCATGGTCGATGGGG
  ACATCCCTCCCAGGGTGAAGAAGGACGCACATGAACTTATCCTGGACTTCATTCGTTCTCGGCCTCCACT
  GAAGCAGGTCTCAGAGAGACAACTTCGCCCTGTACCACAGAAGCAAAGAACACTGCACGAGAAGATCCTG
  GAGGAAATCAAGCAGGAGCGGAGGCTTCGTCCAGTCGGGGCCCAGCACTTGGGAGGCCGTGGGTTTGGTT
  CCCTGCCCTGCATCCTCAATGCCTGCTCTGGGGACATCAAGTCCACTTCCTGCATCAACCTGTCTGTCAC
  CGATACTGGGAGCGGGAGTCAGCGCCCACGGCCCCGGGTCCTGCTCAAAGCCCCCACCCTTGCAGAGATG
  GAAGAAATGAACACGTCTGAGGAAGAAGAGTCCCCATGTGGGGAGGTGGCACTGAAGCGGGACCGCTCTT
  TTTCGGAACACGACCTGGCCCAGCTTCGGAGCGAGATGGCATCTGGCCTTCAGTCAGCCGCTCAGCCCCC
  AGGAGGGACGGAGCCACCTCGGGCCAGAGCAGGTAGCATGCACTCCTGGAGGCCCAGCAGCCGAGACCAG
  GGTTTCTGTCCTGTGAGCGGCCAGTCCCAGCCTCTCCCCAGCTCTGCCTTGCCCAGTAGCTTGAGCTCAG
  TGGATGGACCTGAGGCGGCTTCACCAGATACCAGACACCTGTGGCTGGAGTTCAGCCACCCTGTGGAGAG
  CCTGGCTCTGACTGTGGAAGAGGTGGTGGACGTGCGCAGGGTGCTGGTGAAGGCGGAGATGGAGAGGTTT
  CTGCAGGACAAGGAGCTCTTCAGCAGCCTGAAGAGGGGGAAGATTTGCTGTTGCTGCCGAGCCAAGTTTC
  CCCTGTTTTCCTGGCCACCCACCTGTCTCTTCTGCAAGAGAGCTGTGTGCACTTCCTGTAGCGTAAAGAT
  GAAGATGCCTTCCAAGAAGTATGGACACATCCCTGTCTACACACTTGGCTTTGAGAGTCTTCAGAGGGTG
  CCAACTACCAAAGCCACGCCAACACTGAGGAGAGATGCCTTCCAATCCCTGCAGGGACCAAAGTGGCGGA
  GCGTGGAGGAGGAGTTCCCCCACATCTATGCCCACGGCTGTGTCCTAAAGGATGTCTGCAGTGACTGCAC
  CAGCTTTGTGGCTGATGTCGTGTGCTCCAGCCGCAAGAGCGTGGATGTCCTCAATGCCACTCCACGACGA
  AGCCGTCAGACCCAGTCCCTCTATATCCCCAACACCAGGACTCTCAACTTCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210260 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MARAGGGGAAAPERAGGAARPEPWELSLEEVLKVYEQPINEEQAWAVCFQGCRGLRGEPGGVRRIRDTAD
  ILLRRDGSVGARLEPEPTTMVVPPASSEAQMVQSLGFAIYRALDWGLDENEERELSPQLERLIDLMANSD
  CEDSSCGAADEGYVGPEEEEEAEGGPRAVRTFAQAMRLCALRLTDPHGAQAHYQAVCRALFVETLELRAF
  LARVREAKEMLKKLGEEEPREKPLAELDHLGHTDWARLWVQLMRELRHGVKLKKVQEKEFNPLPTEFQLT
  PFEMLMQDIRARNYKLRKVMVDGDIPPRVKKDAHELILDFIRSRPPLKQVSERQLRPVPQKQRTLHEKIL
  EEIKQERRLRPVGAQHLGGRGFGSLPCILNACSGDIKSTSCINLSVTDTGSGSQRPRPRVLLKAPTLAEM
  EEMNTSEEEESPCGEVALKRDRSFSEHDLAQLRSEMASGLQSAAQPPGGTEPPRARAGSMHSWRPSSRDQ
  GFCPVSGQSQPLPSSALPSSLSSVDGPEAASPDTRHLWLEFSHPVESLALTVEEVVDVRRVLVKAEMERF
  LQDKELFSSLKRGKICCCCRAKFPLFSWPPTCLFCKRAVCTSCSVKMKMPSKKYGHIPVYTLGFESLQRV
  PTTKATPTLRRDAFQSLQGPKWRSVEEEFPHIYAHGCVLKDVCSDCTSFVADVVCSSRKSVDVLNATPRR
  SRQTQSLYIPNTRTLNFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172287
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172287.2, NP_758491.1
• RefSeq Size: 2399 bp
• RefSeq ORF: 2157 bp
• Locus ID: 234857
• UniProt ID: Q8K1S6
• Cytogenetics: 8 E1
• MW: 80.2 kDa
• Gene Summary: Acts as an actin nucleation factor, remains associated with the slow-growing pointed end of the new filament (PubMed:21620703, PubMed:21983562). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning and asymmetric cell division during oocyte meiosis (PubMed:21620703). Required for normal formation of the cleavage furrow and for polar body extrusion during female germ cell meiosis (PubMed:21620703). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity).[UniProtKB/Swiss-Prot Function]