Nckipsd (NM_030729) Mouse Tagged ORF Clone

CAT#: MR210218

Nckipsd (Myc-DDK-tagged) - Mouse NCK interacting protein with SH3 domain (Nckipsd)

Product Images

Circular map for MR210218

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nckipsd
• Synonyms: AF3P21; DIP1; ORF1; SPIN90; Wasbp; WASLBP; WISH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210218 representing NM_030729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGCGCGCTGTATGCGTTCCGCTCCGCGGAGCCGAACGCCATGGCGTTCGCTGCAGGCGAGACCT
  TCCTGGTGCTGGAGCGCAGCAGCACGCACTGGTGGTTAGCAGCACGGGCGCGCAGTGGCGAGACCGGGTA
  CGTGCCGCCTGCCTACCTGCATCGCCTGCAGGGCATGGAACAAGATGTCCTCCAAGCTATTGACCGTGCC
  ATTGAGGCTGTGCACAACACAGCCATGCGAGATGGCGGCAAGTACAGCCTGGAACAGCGTGGAGTCCTTC
  AGAAGCTTATCCATCACCGGAAAGAAACCTTGTCTCGAAGAGGCACCTCAGCTTCCAGTGCTACGGTTAT
  GACCCCATCCACCAGTGACCACCATTTGGACGCTGCTGTATCTAGACAGCCCAATGGGGTGTGTCGAACT
  GGGTTTGAACGGCAGCATAGCCTGCCCAGTTCTGAGCATCTTGGGACAGATGGAGCCCTCTACCAGGTTC
  CACCACAGCCTCGACGAGCAGCTCCTACCACCCCACCCCCACCGGTGAAGCGCCGAGACCGTGAGGCCCT
  GGTGATCTCAGGGAGTGGTGGCCGCACAGCCATACCCTCTGGAGGTAGTTCTGTGTCTAGTGGCTCATCA
  GCCAGCAGCACCTCCATGGACACACTCTATACTGGCTCCAGCCCCTCTGAGCTAGGTCCCAGCTGCTCAC
  CCACACCCCCGCCGGTACCTCGCCGAGGGGCCCACACTACTGTGTCCCAGCCCCAGCCCTCTCCCTCCAA
  GGCACCATCCCCTGAGCCCCCTACGGAGGAGGTGGCAGCTGAGACAAACTCAACCCCTGATGACTTGGAA
  GCTCAGGATGCCCTGAGCCCAGAGACCACAGAGGAGAAGGCAGCCGCTGAGACAGTTGTGCCAAGGACCA
  TTGGGGCAGAGCTGATGGAACTCGTGCGGAGAAACACCGGTCTAAGCCATGAGTTATGCCGTGTAGCCAT
  CGGCGTCGTGGTGGGTCACATCCAGGCCACCGTGCCAGCCAGCTCACCTATCATGGAGCAGGTCCTCCTC
  TCGCTGGTAGAAGGCAAGGACCTGAGCACAGCCCTACCCTCAGGGCAGGTCTGCCACGACCAGCAGAGAC
  TGGAAGTGATTTTTGCAGACCTGGCTCGAAGAAAGGATGACGCCCAGCAGCGCAGCTGGGCGCTGTACGA
  GGACGAGGACGTCATCCGCTGCTATCTGGAAGAACTGCTGCACATCCTGACGGATGCAGATCCCGAAGTT
  TGCAAGAAAATGTGCAAAAGGAGTGATTTTGAGTCTGTCCTAGCCTTGGTGGCCTATTACCAAATGGAAC
  ACCGAGCGTCTCTACGGCTGCTGCTCCTCAAGTGTTTCGGTGCCATGTGCAGCCTAGATGCAGCGATCAT
  CTCCACTCTGGTGTCTTCCGTGCTGCCTGTGGAATTGGCACGGGACATGCAGACCGACACGCAGGACCAC
  CAGAAGCTCTGTTACTCTGCCCTCGTCCTGGCCATGGTCTTCTCCATGGGAGAGGCGGTACCCTACGCAC
  ACTATGAACACCTGGGTACACCCTTTGCCCAGTTCCTACTAAGCATCGTTGAGGATGGCCTTCCAATGGA
  CACCACGGAGCAGCTGCCAGACCTCTGCATGAACCTGCTTCTGGCTCTCAACCTGCACCTGACAGCTCCT
  GAGCAGAATGTCATCATGGCTGCCTTGAGCAGACACACCAATGTGAAGATCTTCTCTGAGAAGCTGCTTC
  TGCTTCTGAACAGAGGGGACGACCCTGTGCGTATCTTCAGACATGAGCCCCAGCCACCACACTCTGTCCT
  CAAGTTCCTGCAGGATGTGTTCAGCAGCTCTGCCACAGCCGCCATCTTCTACCACACAGACATGATGGCG
  CTTATTGACATCACTGTGCGCCAGATCGCAGACCTGTCACCTGGAGACAAGCTACGCATGGAGTACCTCT
  CCCTGATGCATGCTGTGGTCCGCTCCACGCCCTACCTGCAGCACCGACATCGGCTGTCAGACCTGCAGGC
  TACACTGCGGCGCATCCTGACTGAGGAAGAGGCCTCACCCCAGTGCCAGATGGACCGTATGATTGTCCAA
  GAGATGTACAAGGAATTCCCGGACCTGGGAGAGGTTCCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210218 representing NM_030729
  Red=Cloning site Green=Tags(s)
  
  MYRALYAFRSAEPNAMAFAAGETFLVLERSSTHWWLAARARSGETGYVPPAYLHRLQGMEQDVLQAIDRA
  IEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSASSATVMTPSTSDHHLDAAVSRQPNGVCRT
  GFERQHSLPSSEHLGTDGALYQVPPQPRRAAPTTPPPPVKRRDREALVISGSGGRTAIPSGGSSVSSGSS
  ASSTSMDTLYTGSSPSELGPSCSPTPPPVPRRGAHTTVSQPQPSPSKAPSPEPPTEEVAAETNSTPDDLE
  AQDALSPETTEEKAAAETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQATVPASSPIMEQVLL
  SLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEV
  CKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDH
  QKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPMDTTEQLPDLCMNLLLALNLHLTAP
  EQNVIMAALSRHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSSATAAIFYHTDMMA
  LIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEEASPQCQMDRMIVQ
  EMYKEFPDLGEVPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030729
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030729.4, NP_109654.2
• RefSeq Size: 2870 bp
• RefSeq ORF: 2145 bp
• Locus ID: 80987
• UniProt ID: Q9ESJ4
• Cytogenetics: 9 F2
• MW: 78.6 kDa
• Gene Summary: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.[UniProtKB/Swiss-Prot Function]