Uvrag (NM_178635) Mouse Tagged ORF Clone

CAT#: MR210112

Uvrag (Myc-DDK-tagged) - Mouse UV radiation resistance associated gene (Uvrag)

Product Images

Circular map for MR210112

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uvrag
• Synonyms: 9530039D02Rik; AI648770; BB124205; Uvrag1; Uvragl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210112 representing NM_178635
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCTGCGCCTCGCTCGGGGGCCCGGTGCCCCTGCCACCCCCGGGCCCGTCCGCCGCGCTGACGT
  CCGGTGCCCCCGCGCGGGCCCTGCATGTGGAGCTGCCGTCTCAGCAGCGGCGTCTCCGACATCTCCGGAA
  CATCGCTGCTCGGAACATTGTTAATAGAAATGGCCATCAGCTTCTGGATACCTACTTCACTCTTCATTTG
  TGTGATAATGAGAAAATCTTTAAAGAATTTTATAGGAGTGAAGTGATTAAGAATTCCTTGAATCCGACGT
  GGAGGAGTCTTGACTTTGGAATAATGCCGGATCGTCTGGATACATCTGTGTCCTGCTTTGTGGTGAAGAT
  TTGGGGTGGGAAGGAAGAGGCCTTTCAGCTGTTGATAGAGTGGAAAGTCTACCTGGATGGGCTGAAGTAC
  TTGGGACAGCAGATCCATGCCCGCAACCAGAATGAAATCATTTTTGGGCTGAATGATGGCTACTACGGTG
  CTCCCTGTGAACACAAGGGTCATCCAAACGCACAGAAGAACCTCCTTCAGGTGGACCAGAACTGTGTTCG
  CAATTCTTACGATGTCTTCTCTTTGCTGCGGCTTCATAGAGCCCAGTGTGCAATTAAACAGACTCAGGTA
  ACTGTTCAGAGACTTGGAAAGGAAATTGAAGAAAAACTAAGACTCACATCGACAAGCAATGAGCTGAAAA
  AAGAAAGTGAATGTCTGCGATTGAAAATTTTGGTGCTTCGAAATGAACTGGAAAGACAGAAGAAAGCGCT
  GGGGCGGGAGGTGGCCTTCCTGCATAAGCAACAGATGGCTCTACAGGACAAAGGAAGTGCATTTTCCACT
  GAGCATGGCAAGCTCCAGCTGCAGAAGGACTCCCTGAGTGAGCTGAGGAAGGAGTGTACTGCCAAAAGGG
  AACTCTTTCTGAAGACTAATGCTCAGTTGACCATCCGCTGCAGGCAGCTACTCTCAGAGCTGTCCTACAT
  TTACCCTATTGATTTGAATGAGCACAAGGATTATTTTGTATGTGGTGTCAAGTTGCCTAATTCCGAGGAC
  TTCCAAGCAAAAGAGGATGGAAGCATTGCCGTCGCCCTTGGTTACACTGCACATTTGGTCTCCATGATCT
  CCTTCTTCCTACAAGTACCCCTCAGGTATCCCATAATTCACAAGGGGTCCAGATCAACAATCAAAGATAA
  CATCAACGACAAGCTGACAGAAAAGGAGCGAGAGTTTCCACTGTATCCAAAGGGAGGGGAGAAGCTGCAG
  TTTGACTATGGTGTCTACCTTCTGAACAAGAACATAGCACAGCTGAGATACCAGCATGGCCTGGGGACTC
  CAGACTTGAGGCAAACTCTTCCTAACCTGAAAAACTTCATGGAGCACGGACTGATGGTCAGGTGTGACAG
  ACATCACATCTCCAATGCCATCCCTGTCCCGAAGAGACAAAGCTCCACGTTTGGGGGTGCAGATGGAGGC
  TTCTCTGCGGGTATCCCATCACCAGATAAAGTCCATCGCAAGCGGGCCAGCTCAGAGAACGAGAGACTCC
  AGTACAAAACCCCACCTCCCAGCTACAATTCAGCATTGACACAGCCCGGGGTGGCCATGCCCACCTCGGG
  AGACTCTGAGAGAAAGGTCGCCCCTCTGTCCTCCTCCCTGGACACCTCCCTGGACTTCTCCAAAGAAAAC
  AAGAAAGCAGGAGTAGACCTGGGCAGCAGTGTGAGCGGAGACCACGGAAACTCGGACTCCGGCCAAGAGC
  AGGGGGAAGCCCTGCCTGGGCACCTGGCAGCCGTGAATGGCACTGCACTGCCCAGCGAGCAGGCTGGCCC
  TGCCGGCACCCTGCTGCCAGGCTCGTGCCACCCGGCCCCCTCAGCTGAGCTCTGCTGTGCAGTGGAGCAA
  GCAGAGGAAATCATCGGGCTGGAAGCCACAGGCTTCACCTCGGGTGACCAGCTGGAGGCGCTCAGCTGCA
  TCCCGGTGGACAGCGCAGTGGCAGTGGAGTGTGACGAGCAGGTGCTGGGGGAGTTTGAGGAGTTCTCTCG
  CAGGATCTATGCCCTCAGCGAGAACGTGTCCAGCTTCCGCCGGCCTCGCAGGAGTTCTGACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210112 representing NM_178635
  Red=Cloning site Green=Tags(s)
  
  MSSCASLGGPVPLPPPGPSAALTSGAPARALHVELPSQQRRLRHLRNIAARNIVNRNGHQLLDTYFTLHL
  CDNEKIFKEFYRSEVIKNSLNPTWRSLDFGIMPDRLDTSVSCFVVKIWGGKEEAFQLLIEWKVYLDGLKY
  LGQQIHARNQNEIIFGLNDGYYGAPCEHKGHPNAQKNLLQVDQNCVRNSYDVFSLLRLHRAQCAIKQTQV
  TVQRLGKEIEEKLRLTSTSNELKKESECLRLKILVLRNELERQKKALGREVAFLHKQQMALQDKGSAFST
  EHGKLQLQKDSLSELRKECTAKRELFLKTNAQLTIRCRQLLSELSYIYPIDLNEHKDYFVCGVKLPNSED
  FQAKEDGSIAVALGYTAHLVSMISFFLQVPLRYPIIHKGSRSTIKDNINDKLTEKEREFPLYPKGGEKLQ
  FDYGVYLLNKNIAQLRYQHGLGTPDLRQTLPNLKNFMEHGLMVRCDRHHISNAIPVPKRQSSTFGGADGG
  FSAGIPSPDKVHRKRASSENERLQYKTPPPSYNSALTQPGVAMPTSGDSERKVAPLSSSLDTSLDFSKEN
  KKAGVDLGSSVSGDHGNSDSGQEQGEALPGHLAAVNGTALPSEQAGPAGTLLPGSCHPAPSAELCCAVEQ
  AEEIIGLEATGFTSGDQLEALSCIPVDSAVAVECDEQVLGEFEEFSRRIYALSENVSSFRRPRRSSDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178635
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178635.2, NP_848750.2
• RefSeq Size: 3432 bp
• RefSeq ORF: 2097 bp
• Locus ID: 78610
• UniProt ID: Q8K245
• Cytogenetics: 7 E1
• MW: 78 kDa
• Gene Summary: Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex; the function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns(3)P). During autophagy acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. Activates lipid kinase activity of PIK3C3. Involved in the regulation of degradative endocytic trafficking and cytokinesis, and in regulation of ATG9A transport from the Golgi to the autophagosome; the functions seems to implicate its association with PI3KC3-C2. Involved in maturation of autophagosomes and degradative endocytic trafficking independently of BECN1 but depending on its association with a class C Vps complex (possibly the HOPS complex); the association is also proposed to promote autophagosome recruitment and activation of Rab7 and endosome-endosome fusion events. Enhances class C Vps complex (possibly HOPS complex) association with a SNARE complex and promotes fusogenic SNARE complex formation during late endocytic membrane fusion. In case of negative-strand RNA virus infection is required for efficient virus entry, promotes endocytic transport of virions and is implicated in a VAMP8-specific fusogenic SNARE complex assembly.[UniProtKB/Swiss-Prot Function]