Tmem168 (NM_028990) Mouse Tagged ORF Clone

CAT#: MR210103

Tmem168 (Myc-DDK-tagged) - Mouse transmembrane protein 168 (Tmem168)

Product Images

Circular map for MR210103

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmem168
• Synonyms: 5730526F17Rik; 8430437G11Rik; AI462344; AI504145
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210103 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAGATCACTGCGATACTGTGTTAGTCATTGTCTCTATCTAGCAATGACTAGGCTGGAAGAAGTAA
  ATAGAGAAGTGAACATGCATTCCTCCGTGCGGTACCTCGGCTATCTAGCCAGGATCAACTTACTTGTGGC
  CATATGTTTAGGCCTTTATGTAAGATGGGAAAAAACAGCAAATTCCTTAATTTTGGTGATTTTTATCCTC
  GGCCTCTTTGTTCTTGGGATCGCCAGCATACTCTACTATTACTTTTCAATGGAAGCAGCCAGTTTAAGTC
  TCTCCAATCTCTGGTTTGGATTTCTGCTTGGCCTTCTGTGTTTTCTTGATAATTCATCCTTTAAAAGTGA
  TGTGAAAGAAGAAACAACCAAATATTTGCTTCTGACATCTATAGTTTTAAGGATATTGTGTGCTTTGGTG
  GAGAGAATTTCTGGATATGTCCGCCATCGGCCTACTTTACTAACCACAGTGGAGTTTCTGGAACTTGTTG
  GGTTTGCCATCGCCAGCACAACTATGTTGGTGGAGAAGTCTCTGAGTGTTATTTTGCTGGTTATGGCTTT
  GGCCATGCTCATTATCGACCTGAGAATGAAGTCTTTTCTAGCTATTCCAAACTTAATCATTTTTTCAGTA
  TTGTTATTTTTCTCTTCATTGGAAACTCCCCAAAATCCAATTGCTTTTGCATGTTTTTTTATTTGCCTGG
  TAACTGATCCTTTTCTTGACATTTATTTTAGTGGACTTTCAGTAACTGAGAGGTGGAAACCTTTTCTGCA
  TCGTGGGAGAATTTGCAGGAGACTTTCTGTGCTTTTCACTGCAATGATTGAGCTTACATTCTTCATCCTT
  TCAGCATTTAAACTTAGAGACACTCATCTCTGGTATTTTGTGATACCAGGTTTTTCCATTTTCGGATTTT
  TCTGGATGATTTGCCATATTATTTTTCTTTTAACTCTTTGGGGATTTCATACTAAGTTGAATGACTGCCA
  TAAAGTGTATATCAACCACAGGGCAGATAACAATAGCCTTGACAGAATCATGGCATCCAAAGGGATGCGT
  CATTTTTGCTTAATTTCAGAGCAGTTAGTTTTTTTTAGTCTTCTTGCAACGGCAATTTTGGGAGCAGTCT
  CCTGGCAGCCAACCAATGGAATCTTCTTGAGCATGTTTCTCATTGTCTTGCCGCTGGAGTCCATGGCTCA
  TGGGCTCTTCCATGAACTTGGCAACTGCTTAGGGGGAACCTCTGTTGGATACGCTATTGTGATCCCTACC
  AACTTCTGCAGCCCTGATGGCCAGCCAACACTGCTTCCGCCAGAACATGTACAGGAGTTAAATTTGAGGT
  CTACTGGCATGCTCAATGCCATCCAAAGATTTTTTGCATATCATATGATTGAGACCTATGGATGTGACTA
  TTCCACAAGTGGGCTGTCTTTTGATACTCTGCATTCCAAACTGAAAGCTTTCCTTGAACTTCGGACAGTG
  GATGGACCTAGACATGATACATATGTTTTATATTACAGTGGACACACCCATGGCTCAGGAGAGTGGGCCC
  TCGCAGGTGGAGACATACTTCGCCTTGATACGCTTTTAGAATGGTGGAGAGAGAAGAACGGTTCCTTCTG
  TTCCCGGCTTATCATCATCTTAGACAGCGAGAATTCAACCCCATGGGTGAAAGAAGTGAGAAAGATTAAT
  GACCAGTATGTTGCAGTTCAGGGAGCTGAGCTGGCGAAGACAGTAGATATTGAAGAAGCTGACCCGCCAC
  AGCTGGGTGACTTTACTAGAGACTGGGTAGAATACAACTGTAACTCCACAAACAACATCTGCTGGACTGA
  GAAGGGGCGCACAGTGAGGGCCGTGTATGGCGTGTCAAAGCGCTGGAGCGACTACACGCTGCACCTGCCG
  ACAGGAAGCGATGTGGCCAAGCACTGGATGTTACACTTCCCTCGGGTTACGTATCCGTTAGTGCACTTGG
  CCAACTGGTTATGTGGCCTGAACCTCTTTTGGGTCTGCAAAGCTTGTTTTAGGTGCTTGAAAAGGTTAAA
  AATGAGTTGGTTTCTTCCTACAGTGCTGGACACAGGACAAGGCTTTAAACTTGTCAAATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210103 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCRSLRYCVSHCLYLAMTRLEEVNREVNMHSSVRYLGYLARINLLVAICLGLYVRWEKTANSLILVIFIL
  GLFVLGIASILYYYFSMEAASLSLSNLWFGFLLGLLCFLDNSSFKSDVKEETTKYLLLTSIVLRILCALV
  ERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSVILLVMALAMLIIDLRMKSFLAIPNLIIFSV
  LLFFSSLETPQNPIAFACFFICLVTDPFLDIYFSGLSVTERWKPFLHRGRICRRLSVLFTAMIELTFFIL
  SAFKLRDTHLWYFVIPGFSIFGFFWMICHIIFLLTLWGFHTKLNDCHKVYINHRADNNSLDRIMASKGMR
  HFCLISEQLVFFSLLATAILGAVSWQPTNGIFLSMFLIVLPLESMAHGLFHELGNCLGGTSVGYAIVIPT
  NFCSPDGQPTLLPPEHVQELNLRSTGMLNAIQRFFAYHMIETYGCDYSTSGLSFDTLHSKLKAFLELRTV
  DGPRHDTYVLYYSGHTHGSGEWALAGGDILRLDTLLEWWREKNGSFCSRLIIILDSENSTPWVKEVRKIN
  DQYVAVQGAELAKTVDIEEADPPQLGDFTRDWVEYNCNSTNNICWTEKGRTVRAVYGVSKRWSDYTLHLP
  TGSDVAKHWMLHFPRVTYPLVHLANWLCGLNLFWVCKACFRCLKRLKMSWFLPTVLDTGQGFKLVKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028990
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028990.5
• RefSeq Size: 4548 bp
• RefSeq ORF: 2094 bp
• Locus ID: 101118
• UniProt ID: Q91VX9
• Cytogenetics: 6 A1
• MW: 79.7 kDa