Crtc2 (NM_028881) Mouse Tagged ORF Clone

CAT#: MR210074

Crtc2 (Myc-DDK-tagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2)

Product Images

Circular map for MR210074

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crtc2
• Synonyms: 4632407F12Rik; mTORC2; Torc2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210074 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGTCAGGGGCGAACGGGCCGGGTTCCGCCACAGCCTCAGCGTCCAATCCACGCAAGTTTAGTG
  AGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACAT
  CGGCTCCACACGGTTACAGGCCCAAAAACTGCGACTGGCTTATACAAGGAGCTCCCATTATGGTGGTTCT
  CTGCCCAATGTTAACCAGATTGGCTGTGGCCTGGCTGAGTTCCAGAGCCCCCTTCACTCACCTTTGGATT
  CATCTCGGAGCACTCGGCACCATGGGTTGGTGGAACGGGTACAACGAGATGCCCGCAGAATGGTGTCCCC
  ACTCCGCCGATACCCCCGCCACATTGACAGTTCTCCATATAGCCCTGCCTATTTATCTCCTCCCCCAGAG
  TCTGGCTGGCGAAGGATGATGCCCTGGGGCAACCTCCCAGCTGAGAAGGGGCAGTTGTTTAGACTGCCGT
  CTGCACTTAACAGGACAAGCTCTGACTCTGCTCTTCACACAAGTGTGATGAACCCTAACCCCCAAGACAC
  TTATCCAGGCCCCACACCTCCCAGTGTCCTGCCCAGCCGCCGAGGAGGAGGTTTTCTGGATGGTGAGATG
  GATGCTAAAGTCCCTGCTATTGAGGAGAACGTGGTAGATGACAAGCATCTGCTGAAACCTTGGGATGCTA
  AGAAGCTGTCCTCATCTTCCTCTCGACCTCGATCCTGTGAAGTCCCTGGAATCAACATCTTTCCATCTCC
  TGACCAGCCTGCTAATGTGCCTGTCCTCCCACCTGCCATGAACACAGGAGGCTCCCTACCTGACCTCACC
  AACCTACACTTCCCTCCTCCACTGCCCACACCCCTGGACCCTGAGGAGACAGTCTATCCCAGCCTGAGTG
  GGGGCAACAGTACCACCAATTTGACCCATACTATGACCCATTTGGGCATCAGTGGAGGTCTGGGCTTGGG
  CCCCAGCTATGATGTACCAGGACTTCACTCACCTCTTAGCCACCCATCCCTGCAGTCCTCCCTAAGCAAT
  CCCAACCTCCAGGCTTCCCTAAGTAGTCCTCAACCCCAGCTCCAGGGCTCCCATAGTCACCCATCACTGC
  CTGCCTCCTCCTTGGCCCACCATGCACTGCCCACCACCTCCCTGGGCCACCCGTCACTTAGTGCCCCAGC
  CCTTTCCTCCTCCTCTTCCTCTTCGTCCACTTCATCTCCTGTCCTGAGTGCTCCCCCTTACCCAGCTTCT
  ACTCCTGGGGCCTCTCCCCGCCACCGTCGTGTGCCCCTCAGCCCCCTGAGCTTGCCCGCGGGCCCAGCCG
  ACGCCAGAAGGTCCCAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCACCCACCTTGTCTTCCAT
  CACTCAGGGCGTTCCCTTGGATACCAGTAAATTGCCTACTGACCAGCGATTGCCCCCATACCCATATAGC
  CCCCCAAGTCTAGTTATACCCACCCATCCACCCACCCCAAAGTCTCTACAGCAGTTGCCCTCTCAGGCCT
  GCTTAGTGCAGCCCTCAGGTGGACAGCCTCCAGGCAGGCAGCCACATTATGGGGCACTGTACCCACCTGG
  GTCCAGTGGACATGGGCAACAGCCTTACCACCGCCCAATAAATGACTTCAGCTTGGGGAATCTGGAGCAA
  TTCAACATGGAGAGCCCATCAACCAGCCTGGTGCTGGATCCCCCTGCCTTTTCTGAAGGTCCTGGATTTT
  TAGGGAGTGAGGGATCAATGAGTGGCCCCCAGGACCCTCACGTCCTCAACCACCAGAACTTGACCCACTG
  TTCCCGCCATGGCTCAGGACCTAATATCATACTCACAGGAGACTCTTCCCCAGGTTTCTCTAAGGAGATT
  GCAGCAGCCCTGGCTGGAGTGCCTGGCTTTGAGGTGTCAGCATCTGGGTTGGAGCTGGGCCTGGGGCTAG
  AAGATGAGCTGCGCATGGAGCCACTGGGCCTGGAAGGACTAACTATGCTGAGTGACCCCTGTGCCCTGCT
  GCCTGACCCTGCTGTGGAGGACTCATTCCGTAGTGATCGGCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210074 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATSGANGPGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQAQKLRLAYTRSSHYGGS
  LPNVNQIGCGLAEFQSPLHSPLDSSRSTRHHGLVERVQRDARRMVSPLRRYPRHIDSSPYSPAYLSPPPE
  SGWRRMMPWGNLPAEKGQLFRLPSALNRTSSDSALHTSVMNPNPQDTYPGPTPPSVLPSRRGGGFLDGEM
  DAKVPAIEENVVDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFPSPDQPANVPVLPPAMNTGGSLPDLT
  NLHFPPPLPTPLDPEETVYPSLSGGNSTTNLTHTMTHLGISGGLGLGPSYDVPGLHSPLSHPSLQSSLSN
  PNLQASLSSPQPQLQGSHSHPSLPASSLAHHALPTTSLGHPSLSAPALSSSSSSSSTSSPVLSAPPYPAS
  TPGASPRHRRVPLSPLSLPAGPADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLPTDQRLPPYPYS
  PPSLVIPTHPPTPKSLQQLPSQACLVQPSGGQPPGRQPHYGALYPPGSSGHGQQPYHRPINDFSLGNLEQ
  FNMESPSTSLVLDPPAFSEGPGFLGSEGSMSGPQDPHVLNHQNLTHCSRHGSGPNIILTGDSSPGFSKEI
  AAALAGVPGFEVSASGLELGLGLEDELRMEPLGLEGLTMLSDPCALLPDPAVEDSFRSDRLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028881
• ORF Size: 2079 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028881.3
• RefSeq Size: 2647 bp
• RefSeq ORF: 2079 bp
• Locus ID: 74343
• UniProt ID: Q3U182
• Cytogenetics: 3 F1
• MW: 73.2 kDa
• Gene Summary: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites (PubMed:29211348). Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated (PubMed:29211348). Acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells (By similarity).[UniProtKB/Swiss-Prot Function]