Cpsf3 (NM_018813) Mouse Tagged Lenti ORF Clone
CAT#: MR210024L4
- LentiORF®
Lenti ORF clone of Cpsf3 (mGFP-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3)
"NM_018813" in other vectors (4)
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USD 365.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Cpsf3 |
Synonyms | MGC118660 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR210024).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018813 |
ORF Size | 2055 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018813.2, NP_061283.2 |
RefSeq Size | 2560 bp |
RefSeq ORF | 2055 bp |
Locus ID | 54451 |
UniProt ID | Q9QXK7 |
Cytogenetics | 12 A1.3 |
Gene Summary | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3' endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202882 | Cpsf3 (untagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3), (10ug) |
USD 627.00 |
|
MG210024 | Cpsf3 (tGFP-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) |
USD 827.00 |
|
MR210024 | Cpsf3 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) |
USD 627.00 |
|
MR210024L3 | Lenti ORF clone of Cpsf3 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) |
USD 927.00 |
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