Cpsf3 (NM_018813) Mouse Untagged Clone

CAT#: MC202882

Cpsf3 (untagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpsf3
• Synonyms: MGC118660
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023297 sequence for NM_018813
  CGGACGCGTGGGCAGTAGCTGTGCTTTGAATTTAGAGACTGCGGCGACGAGTTCGTGGTCCACGCTGCTC CTGAGACTGCCAGGATGTCTGCGATTCCTGCAGAGGAGAGCGACCAGCTGCTGATCCGACCCCTTGGTGC TGGGCAAGAAGTGGGAAGATCATGTATTATTCTGGAGTTCAAAGGAAGGAAAATCATGCTGGACTGTGGG ATCCACCCTGGCCTGGAAGGAATGGATGCCCTTCCTTACATTGACCTTATCGACCCAGCTGAGATCGACC TTCTGTTGATCAGTCATTTCCATTTGGATCACTGTGGAGCCCTGCCCTGGTTCCTTCAGAAGACAAGTTT CAAAGGACGGACATTTATGACCCACGCCACAAAAGCTATTTATAGATGGCTTCTCTCAGACTATGTCAAA GTCAGCAACATATCGGCAGATGACATGCTGTACACTGAGACAGACTTGGAAGAAAGCATGGACAAAATCG AAACCATCAATTTTCATGAAGTTAAGGAAGTTGCAGGAATCAAGTTCTGGTGTTACCATGCCGGCCACGT CCTGGGAGCAGCCATGTTTATGATAGAGATCGCCGGTGTGAAGCTTTTGTATACAGGTGATTTCTCAAGA CAAGAAGACAGACATCTAATGGCTGCTGAGATTCCTAATATTAAGCCAGACATCCTGATCATTGAGTCTA CGTATGGGACCCATATCCATGAGAAGCGTGAAGAGCGAGAAGCAAGATTCTGCAACACTGTGCACGACAT TGTCAACAGAGGGGGCAGAGGGCTCATTCCTGTCTTTGCTCTCGGAAGAGCTCAGGAGCTGCTCCTGATT CTAGATGAGTACTGGCAGAACCACCCGGAGCTGCATGACATACCCATTTACTACGCATCGTCTCTGGCCA AGAAGTGCATGGCCGTGTACCAGACCTACGTGAATGCCATGAACGACAAGATCCGGAAGCAGATCAACAT CAACAACCCCTTTGTTTTCAAGCACATCAGCAACCTCAAGAGCATGGATCACTTTGATGACATTGGCCCC AGTGTTGTGATGGCCTCACCAGGCATGATTCAAAACGGCTTATCCAGAGAGCTGTTTGAAAGCTGGTGTA CAGATAAGAGGAATGGCGTCATCATCGCGGGGTACTGTGTCGAAGGGACACTTGCCAAGCACATCATGTC TGAACCTGAAGAAATCACGACCATGTCTGGACAGAAGTTGCCACTGAAAATGTCGGTTGATTACATTTCT TTCTCTGCTCACACAGATTACCAGCAAACCAGTGAATTTATTCGTGCTCTGAAGCCACCTCACGTGATTT TAGTCCATGGGGAACAGAATGAAATGGCCAGGCTGAAAGCAGCGCTGATCCGAGAATATGAAGATAATGA TGAAGTTCACATCGAGGTTCACAATCCTCGGAACACAGAGGCCGTGACCTTAAACTTCAGGGGAGAGAAG CTCGCCAAGGTCATGGGCTTTTTGGCAGACAAGAAACCAGAACAGGGCCAGCGGGTCTCTGGAATTCTTG TTAAAAGAAACTTTAATTACCATATACTCTCTCCCTGTGACCTCTCCAATTACACTGACCTGGCCATGAG CACTGTGAAACAGACCCAGGCCATCCCGTACACCGGGCCCTTTTACCTGCTCTACTACCAGCTCCAGAAA TTGACGGGTGATGTAGAAGAATTAGAAATTCAAGAAAAGCCTGCTCTGAAGGTGTTCAAAAGCATCACTG TGGTGCAGGAGCCGGGCATGGTGGTTCTGGAGTGGCTGGCGAACCCCTCTAACGACATGTATGCAGACAC AGTGACCACCGTGATATTGGAAGTTCAGTCAAATCCAAAGATAAGGAAAGGTGCAGTACAGAAGGTCTCT AAGAAGCTAGAGATGCACGTTTACAGCAAGAGGCTGGAGGTCATGCTCCAGGACATATTTGGAGAAGACT GTGTCAGTGTGAAAGATGACTCCGTCCTCAGTGTCACAGTGGACGGGAAAACAGCAAATATTAATCTGGA GACTCGGGCTGTAGAATGTGAAGAAGGAAGTGAAGATGATGAATCCCTCCGAGAAATGGTGGAACTGGCC GCCCAGAGACTGTACGAGGCTCTGACGCCGGTGCACTGACAGCCTTGCCTCCCAGACTCCCAGACTTTTT TACACTCAGCCAAGAGTTAACTTCTGTTCTTCAAATAAAATAACCTTAGTTTCTAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_018813
• Insert Size: 2055 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023297, AAH23297
• RefSeq Size: 2253 bp
• RefSeq ORF: 2055 bp
• Locus ID: 54451
• UniProt ID: Q9QXK7
• Cytogenetics: 12 A1.3
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3' endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.[UniProtKB/Swiss-Prot Function]