Gfpt2 (NM_013529) Mouse Tagged ORF Clone

CAT#: MR210009

Gfpt2 (Myc-DDK-tagged) - Mouse glutamine fructose-6-phosphate transaminase 2 (Gfpt2)

Product Images

Circular map for MR210009

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gfpt2
• Synonyms: AI480523; GFAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210009 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCGGAATCTTTGCCTACATGAATTACAGAGTTCCCAAGACAAGGAAAGAGATTTTCGAAACCCTTA
  TCAGGGGTCTGCAGCGGCTGGAGTACCGGGGCTATGACTCTGCGGGGGTTGCCATTGATGGGAATAACCA
  CGAAGTCAAAGAAAGACACATCCATCTTGTGAAGAAAAGGGGGAAAGTAAAGGCTCTGGATGAAGAACTT
  TACAAGCAAGATAGCATGGACTTGAAGGTGGAGTTTGAGACACACTTCGGCATTGCCCACACACGTTGGG
  CCACCCACGGGGTTCCCAATGCTGTCAACAGTCACCCGCAGCGTTCGGACAAAGACAATGAATTTGTTGT
  CATCCACAACGGGATCATCACTAATTACAAGGATCTAAGGAAGTTTCTGGAAAGCAAAGGCTACGAGTTT
  GAGTCAGAAACAGACACGGAGACCATCGCCAAGCTGATTAAATATGTATTTGACAACAGAGAGACTGAGG
  ACATAACGTTTTCCACATTGGTCGAAAGAGTCATTCAGCAGTTGGAAGGCGCCTTTGCACTGGTTTTCAA
  GAGTATTCACTACCCGGGAGAAGCTGTCGCCACGAGGAGAGGCAGCCCCTTGCTCATCGGGGTACGAAGC
  AAATACAAACTCTCCACAGAGCAGATCCCCGTCTTATATCCGACATGCAATATCGAGAATGTGAAGAATA
  TCTGCAAGACTAGGATGAAGAGACTGGACAGCTCCACCTGCCTGCACGCTGTGGGCGATAAAGCTGTGGA
  ATTCTTCTTTGCTTCTGATGCAAGTGCCATCATAGAACACACCAACCGGGTCATCTTCTTAGAAGATGAT
  GATATCGCTGCAGTGGCTGATGGGAAACTCTCCATTCACCGAGTCAAGCGCTCAGCTACTGATGACCCCT
  CCCGAGCCATCCAGACCTTGCAGATGGAACTGCAGCAAATAATGAAAGGTAACTTCAGCGCATTTATGCA
  GAAGGAGATCTTCGAGCAGCCAGAATCAGTTTTTAATACCATGAGAGGTCGGGTGAATTTTGAGACCAAC
  ACAGTGCTCCTGGGTGGCTTGAAGGACCATTTGAAAGAGATCCGACGATGCCGAAGGCTCATTGTGATTG
  GCTGTGGAACCAGCTACCATGCCGCTGTGGCTACACGGCAAGTCTTAGAGGAACTGACCGAGCTGCCTGT
  GATGGTTGAACTTGCCAGTGACTTTCTGGACAGGAACACACCTGTGTTCAGGGATGACGTTTGCTTTTTC
  ATAAGCCAATCAGGTGAGACTGCAGACACGCTCCTGGCGCTGCGATACTGTAAGGATCGAGGTGCGCTGA
  CCGTGGGCATCACCAACACCGTGGGTAGCTCCATCTCCCGGGAGACTGACTGTGGCGTCCACATCAACGC
  AGGGCCCGAGATTGGGGTGGCCAGCACCAAGGCGTACACCAGCCAGTTCATCTCTCTGGTGATGTTTGGT
  TTGATGATGTCTGAAGATCGAATTTCTCTACAGAACAGGAGACAAGAGATCATCCGTGGCCTCAGATCTT
  TACCGGAGCTGATCAAAGAAGTGCTGTCCCTGGATGAGAAGATCCATGACTTGGCCCTGGAGCTCTACAC
  ACAAAGGTCTCTCCTCGTGATGGGACGGGGATATAACTATGCCACATGTCTGGAAGGTGCCTTGAAAATT
  AAGGAGATAACCTACATGCATTCAGAAGGTATCCTAGCCGGAGAGCTGAAGCACGGGCCCCTTGCTCTCG
  TCGACAAGCAGATGCCAGTCATCATGGTCATCATGAAGGATCCTTGCTTTGCCAAGTGCCAGAATGCCCT
  GCAGCAGGTCACTGCCCGCCAGGGTCGCCCAATCATACTGTGTTCCAAGGATGACACCGAGAGCTCCAAG
  TTTGCATATAAAACCATTGAACTTCCCCACACAGTGGACTGTCTCCAGGGTATCCTGAGCGTGATTCCAC
  TCCAGCTTCTGTCCTTCCACCTGGCTGTCCTCCGAGGTTATGATGTTGACTTCCCCAGAAACCTAGCCAA
  GTCTGTCACTGTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210009 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCGIFAYMNYRVPKTRKEIFETLIRGLQRLEYRGYDSAGVAIDGNNHEVKERHIHLVKKRGKVKALDEEL
  YKQDSMDLKVEFETHFGIAHTRWATHGVPNAVNSHPQRSDKDNEFVVIHNGIITNYKDLRKFLESKGYEF
  ESETDTETIAKLIKYVFDNRETEDITFSTLVERVIQQLEGAFALVFKSIHYPGEAVATRRGSPLLIGVRS
  KYKLSTEQIPVLYPTCNIENVKNICKTRMKRLDSSTCLHAVGDKAVEFFFASDASAIIEHTNRVIFLEDD
  DIAAVADGKLSIHRVKRSATDDPSRAIQTLQMELQQIMKGNFSAFMQKEIFEQPESVFNTMRGRVNFETN
  TVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFF
  ISQSGETADTLLALRYCKDRGALTVGITNTVGSSISRETDCGVHINAGPEIGVASTKAYTSQFISLVMFG
  LMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLDEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKI
  KEITYMHSEGILAGELKHGPLALVDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSK
  FAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013529
• ORF Size: 2049 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013529.3
• RefSeq Size: 2986 bp
• RefSeq ORF: 2049 bp
• Locus ID: 14584
• UniProt ID: Q9Z2Z9
• Cytogenetics: 11 29.9 cM
• MW: 77 kDa
• Gene Summary: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.[UniProtKB/Swiss-Prot Function]