Ift81 (NM_009879) Mouse Tagged ORF Clone

CAT#: MR209962

Ift81 (Myc-DDK-tagged) - Mouse intraflagellar transport 81 homolog (Chlamydomonas) (Ift81)

Product Images

Circular map for MR209962

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ift81
• Synonyms: AW060663; Cdv-1; Cdv-1r; Cdv1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209962 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGACCAAATCAAATTCATCGTGGACAGCCTCAATAAGGAGCCTTTTAAGAAGAATTATAACTTAA
  TCACATTTGATTCTTTGGGACCAATGCAACTATTGCAAGTTCTCAATGATGTCCTGGCTGAGATTGACCC
  CAAGCAAGATGTTGATATCAGAGAGGAGATGCCAGAGCAGACAGCCAAACGAATGTTGAATCTTCTTGGT
  ATCCTTAAGTACAAACCTCCGGGAAATGCCACAGACATGAGCACCTTCCGTCAGGGCTTGGTGATTGGAA
  GTAAACCTGTGATTTACCCAGTGCTCCACTGGCTTCTTCAGCGGAGTAATGAACTAAAGAAAAGGGCATA
  TTTAGCTCGTTTCTTAATAAAACTGGAGGTGCCAAGTGAGTTTCTTCAAGATGAGACCGTGGCTGACACC
  AATAAACAGTATGAAGAGCTGATGGAAGCCTTTAAAACTCTGCATAAAGAGTGTGAGCAGCTCAAAACAT
  CTGGATTCTCTACAGCAGAAATCAGAAGGGATATCAGCGCAATGGAGGAGGAAAAAGACCAGCTCATGAA
  GAGAGTTGAACGGCTGAAGAAGAGGGTGGAGACCGTTCAGAACCATCAGCGAATGCTTAAAATAGCAAGA
  CAGCTTCGAGTTGAGAAAGAGAGAGAAGAATTTCTTGCACAACAGAAACAGGAACAAAAGAATCAGCTGT
  TTCATGCGGTGCAGAGATTGCAGAGAGTGCAAAATCAGCTGAAGAGCATGCGGCACGCAGCAGCGGATGC
  AAAGCCTGAGAGTTTAATGAAGAGGCTAGAGGAGGAGATAAAATTTAACTCATACATGGTCACTGAAAAA
  TTTCCAAAAGAATTAGAAAGCAAGAAAAAGGAATTACATTTTTTACAGAAGGTGGTTTCTGAGCCAGCTA
  TGGGCCACTCTGACCTTCTCGAACTTGAGACTAAAGTCAATGAAGTAAACACGGAGATCAATCAGTTGAT
  TGAAAAGAAAATGATGAGAAACGAGCCAATTGAAGGCAAGCTCTCACTGTACAGGCAACAGGCATCCATC
  ATTTCTCGTAAGAAAGAAGCCAAAGCTGAGGAGCTGCAGGAGACCAAGGAGAAGCTGGCCAGCCTTGAGA
  GAGAGGTGTTGGTGAAAACAAACCAGACCCGGGAGTTCGACGGCACTGAGGTTCTGAAGGGAGATGAGTT
  CAAGCGATACGTCAGTAAACTTCGAAGCAAAAGCACAGTTTTCAAAAAGAAGCATCAGATAATAGCTGAA
  TTTAAGGCTGAGTTTGGCCTCCTGCAGAGGACAGAAGAACTTCTGAAGCAGCGTCAGGAAACCATTCAGC
  ATCAACTGCGAACTATTGAAGAAAAGAAGGGTATATCTGGATACAGTTACACCCAAGAGGAGCTTGAGAG
  AGTGTCGGCCCTGAAGAGTGAGGTTGACGAGATGAAAGGCCGGACCCTGGATGACATGTCAGAGATGGTG
  AAAAAACTGAATTCCTTGGTATCAGAAAAGAAGTCAGCTTTAGCCCCAGTTATAAAAGAACTACGGCAGT
  TGCGTCAGAAATGTCAAGAACTAACCCAGGAGTGTGATGAGAAGAAAGCCCAGTATGATAGCTGTGCAGC
  AGGCCTTGAAAGCAACCGGTCCAAATTAGAGCAGGAAGTGCGAGGACTTCGTGAGGAATGTCTTCAAGAA
  GAAAGTAAATACCACTATACAAACTGCATGATTAAGAACCTAGAAGTTGAGCTTCGTCGAGCGACTGATG
  AGATGAAGGCGTACGTCTCTTCTGATCAGCAAGAGAAGAGGAAAGCAATTAGGGAACAGTATACCAAAAA
  TATTACTGAGCAAGAAAACCTTGGTAAGAAACTCCGGGAAAAACAAAAAGCTGTACGAGAAAGTCATGGT
  CCAAACATGAAGCAAGCAAAAATGTGGCGTGATCTTGAACAACTGATGGAATGCAAAAAACAGTGCTTTC
  TAAAGCAGCAAAGCCCAGCTTCCATCGGCCAGGTAATTCAAGAGGGCGGAGAGGACCGGCTGGTTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209962 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDQIKFIVDSLNKEPFKKNYNLITFDSLGPMQLLQVLNDVLAEIDPKQDVDIREEMPEQTAKRMLNLLG
  ILKYKPPGNATDMSTFRQGLVIGSKPVIYPVLHWLLQRSNELKKRAYLARFLIKLEVPSEFLQDETVADT
  NKQYEELMEAFKTLHKECEQLKTSGFSTAEIRRDISAMEEEKDQLMKRVERLKKRVETVQNHQRMLKIAR
  QLRVEKEREEFLAQQKQEQKNQLFHAVQRLQRVQNQLKSMRHAAADAKPESLMKRLEEEIKFNSYMVTEK
  FPKELESKKKELHFLQKVVSEPAMGHSDLLELETKVNEVNTEINQLIEKKMMRNEPIEGKLSLYRQQASI
  ISRKKEAKAEELQETKEKLASLEREVLVKTNQTREFDGTEVLKGDEFKRYVSKLRSKSTVFKKKHQIIAE
  FKAEFGLLQRTEELLKQRQETIQHQLRTIEEKKGISGYSYTQEELERVSALKSEVDEMKGRTLDDMSEMV
  KKLNSLVSEKKSALAPVIKELRQLRQKCQELTQECDEKKAQYDSCAAGLESNRSKLEQEVRGLREECLQE
  ESKYHYTNCMIKNLEVELRRATDEMKAYVSSDQQEKRKAIREQYTKNITEQENLGKKLREKQKAVRESHG
  PNMKQAKMWRDLEQLMECKKQCFLKQQSPASIGQVIQEGGEDRLVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009879
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009879.4
• RefSeq Size: 2957 bp
• RefSeq ORF: 2031 bp
• Locus ID: 12589
• UniProt ID: O35594
• Cytogenetics: 5 62.44 cM
• MW: 79.3 kDa
• Gene Summary: Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling.[UniProtKB/Swiss-Prot Function]