Slc5a2 (NM_133254) Mouse Tagged ORF Clone

CAT#: MR209920

Slc5a2 (Myc-DDK-tagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 2 (Slc5a2)

Product Images

Circular map for MR209920

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc5a2
• Synonyms: Sglt2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209920 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAACACGTAGAGGCAGGCTCTGAACTTGGGGAGCAGAAGGTCCTGATTGATAATCCTGCTGACA
  TTCTGGTTATCGCTGCCTATTTCCTGCTGGTCATTGGTGTTGGCTTGTGGTCTATGTTCAGAACCAATAG
  AGGCACAGTTGGTGGCTACTTCCTGGCAGGACGGAGCATGGTGTGGTGGCCGGTTGGAGCCTCTCTGTTC
  GCCAGCAACATCGGCAGCGGTCATTTTGTGGGCCTGGCAGGGACTGGCGCAGCAAGTGGCTTGGCGGTGG
  CTGGATTTGAGTGGAATGCGCTCTTCGTGGTGCTGCTCCTCGGATGGCTTTTTGTGCCAGTGTATCTGAC
  CGCTGGTGTGATCACAATGCCTCAGTACCTCCGCAAGCGCTTTGGTGGGCACCGTATTCGCCTCTACCTG
  TCCGTGCTCTCGCTTTTTTTGTACATTTTCACCAAGATCTCGGTGGATATGTTCTCTGGGGCAGTATTCA
  TTCAACAGGCCCTGGGCTGGAACATTTACGCTTCGGTCATCGCTCTCTTGGGCATCACCATGATTTATAC
  TGTGACAGGAGGGCTGGCGGCACTGATGTACACAGACACTGTGCAGACCTTCGTCATTCTTGCCGGGGCC
  TTCATCCTCACTGGTTATGCTTTCCATGAAGTGGGCGGGTACTCGGGTCTCTTCGACAAATACCTGGGAG
  CAATGACTTCACTGACGGTGTCCAAGGATCCATCTGTTGGCAACATCTCCAGCACCTGCTACCAGCCGAG
  GCCTGACTCCTATCACCTGCTGCGTGACCCTGTGACAGGAGACCTGCCATGGCCTGCGCTGCTCCTGGGG
  CTTACCATTGTCTCGGGCTGGTATTGGTGCAGCGATCAGGTAATAGTGCAGCGGTGCCTGGCTGGAAAGA
  ATCTGACTCACATCAAAGCTGGGTGCATCTTGTGTGGCTACCTGAAGCTGATGCCCATGTTCCTCATGGT
  CATGCCAGGCATGATCAGCCGCATTCTCTACCCAGATGAGGTGGCATGTGTGGTACCTGAGGTGTGTAAG
  CGGGTGTGTGGCACTGAGGTGGGCTGCTCTAACATCGCCTACCCACGGCTCGTGGTGAAGCTCATGCCCA
  ATGGTCTGCGCGGACTCATGCTGGCAGTCATGCTGGCTGCCCTCATGTCTTCTCTGGCATCCATCTTTAA
  CAGCAGTAGCACGCTCTTCACCATGGATATCTACACGCGCCTGCGGCCCCGTGCAGGTGATAAGGAGCTG
  CTGCTAGTTGGAAGGCTCTGGGTGGTATTCATCGTGGCGGTGTCCGTGGCTTGGCTGCCAGTGGTGCAGG
  CAGCTCAGGGTGGGCAGCTCTTCGATTACATTCAGTCTGTCTCCAGCTATCTGGCACCTCCAGTGTCTGC
  GGTCTTTGTGCTTGCACTCTTTGTGCCCCGTGTTAATGAGAAGGGAGCCTTCTGGGGACTAGTTGGGGGC
  CTGCTGATGGGCCTAGCTCGTCTCATACCCGAGTTCTTCTTTGGCTCGGGCAGCTGTGTGCGACCCTCAG
  CGTGCCCGGCACTCTTCTGTCGGGTACACTACCTCTATTTCGCCATCATTCTCTTCATCTGCTCTGGCAT
  CCTCACACTTGGAATCTCCCTGTGCACTGCGCCCATCCCTCAGAAGCATCTCCATCGCCTGGTTTTCAGT
  CTCCGGCACAGCAAGGAGGAGCGGGAGGACCTGGATGCTGATGAGTTAGAAGGTCCAGCCCCTGCTCCTG
  TGCAGAACGGGGGCCAGGAATGTGCAATGGAGATGGAAGAGGTCCAGTCCCCGGCTCCAGGCCTGCTCCG
  CCGGTGCCTGCTTTGGTTCTGTGGGATGAGCAAGAGTGGGTCAGGGAGTCCTCCGCCCACTACCGAGGAG
  GTGGCGGCAACCACCAGGCGGCTGGAGGACATCAGTGAGGATCCCCGCTGGGCACGAGTAGTCAACCTCA
  ATGCCCTACTCATGATGACCGTGGCTGTGTTCCTCTGGGGCTTCTATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209920 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQHVEAGSELGEQKVLIDNPADILVIAAYFLLVIGVGLWSMFRTNRGTVGGYFLAGRSMVWWPVGASLF
  ASNIGSGHFVGLAGTGAASGLAVAGFEWNALFVVLLLGWLFVPVYLTAGVITMPQYLRKRFGGHRIRLYL
  SVLSLFLYIFTKISVDMFSGAVFIQQALGWNIYASVIALLGITMIYTVTGGLAALMYTDTVQTFVILAGA
  FILTGYAFHEVGGYSGLFDKYLGAMTSLTVSKDPSVGNISSTCYQPRPDSYHLLRDPVTGDLPWPALLLG
  LTIVSGWYWCSDQVIVQRCLAGKNLTHIKAGCILCGYLKLMPMFLMVMPGMISRILYPDEVACVVPEVCK
  RVCGTEVGCSNIAYPRLVVKLMPNGLRGLMLAVMLAALMSSLASIFNSSSTLFTMDIYTRLRPRAGDKEL
  LLVGRLWVVFIVAVSVAWLPVVQAAQGGQLFDYIQSVSSYLAPPVSAVFVLALFVPRVNEKGAFWGLVGG
  LLMGLARLIPEFFFGSGSCVRPSACPALFCRVHYLYFAIILFICSGILTLGISLCTAPIPQKHLHRLVFS
  LRHSKEEREDLDADELEGPAPAPVQNGGQECAMEMEEVQSPAPGLLRRCLLWFCGMSKSGSGSPPPTTEE
  VAATTRRLEDISEDPRWARVVNLNALLMMTVAVFLWGFYA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133254
• ORF Size: 2013 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133254.5
• RefSeq Size: 2259 bp
• RefSeq ORF: 2013 bp
• Locus ID: 246787
• UniProt ID: Q923I7
• Cytogenetics: 7 F3
• MW: 73 kDa
• Gene Summary: Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.[UniProtKB/Swiss-Prot Function]