Mtmr1 (NM_016985) Mouse Tagged ORF Clone

CAT#: MR209913

Mtmr1 (Myc-DDK-tagged) - Mouse myotubularin related protein 1 (Mtmr1)

Product Images

Circular map for MR209913

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mtmr1
• Synonyms: AW049210
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209913 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGGCCAGTGGCGGCGGCGGCCGCCGCATCTGCGGCGAGCTGCGAGGGCGCTGGGGGCCCGGGGC
  CAGGGCCGGGCGCTAGCTGGAGACCCTCTCGGGTTGCGGGGGGCGCCTCGGCCAGCTCCCGGCACCCCAG
  CATTGAGACCCTTGACAGTCCTACAGGGTCACATGTTGAATGGTGTAAACAGCTTATAGCTGCTACAATT
  TCTAGTCAGATTTCAGGTTCAGTGACATCAGAAAATGTGTCAAGAGATTACAAGGCTCTAAGGGATGGAA
  ATAAACTGGCACAGATGGAAGAAGCTCCACTTTTCCCAGGAGAATCAATTAAGGCCATTGTGAAAGACGT
  CATTTATATCTGTCCATTTATGGGAGCAGTGAGTGGGACCCTGACAGTGACGGACTTTAAAATGTACTTC
  AAAAATGTAGAAAGGGACCCACATTTCGTCCTTGATGTTCCTCTTGGAGTGATCAGCAGAGTGGAGAAGA
  TTGGTGCACAGAGCCATGGGGATAATTCTTGTGGTATAGAGATTGTGTGCAAGGATATGAGGAACCTGAG
  ACTTGCATATAAGCAGGAAGAACAGCGAAAACTTGGGATATTTGAAAACCTCAACAAGCATGCTTTTCCT
  CTTTCCAATGGGCAGGTGCTATTTGCATTCAACTATAAAGAAAAGTTTCCAGTTAATGGCTGGAAAGTTT
  ATGATCCAGTGTCTGAATATAAGAGACAGGGCTTGCCAAATGAGAGTTGGAAAATATCAAAAATAAACAG
  TAATTATGAGTTCTGTGATACCTATCCTGCCATCATTGTTGTGCCAACTAGTGTGAAAGATGATGACCTT
  TCAAAAGTGGCAGCCTTTCGAGCGAAAGGCAGAGTCCCTGTGTTGTCATGGATTCACCCAGAAAGTCAGG
  CAACAATTACACGTTGCAGCCAGCCATTAGTGGGTCCCAATGATAAACGCTGCAAAGAAGATGAGAAATA
  CCTGCAGACAATAATGGATGCTAATGCACAGTCACATAAACTTACTATCTTTGATGCCAGACAAAACAGC
  GTTGCTGATACCAACAAGGCAAAGGGTGGTGGATATGAAAATGAAAGTGCTTACCCTAATGCCGAACTCA
  TATTCTTGGAGATCCACAATATTCATGTGATGAGGGAGTCACTTCGTAAATTAAAGGAGATTGTGTATCC
  TTCAATTGATGAGTCACATTGGCTATCCAATGTGGATGGGACACATTGGCTGGAATATATACGGGTTCTC
  CTTGCTGGGGCAGTGAGAATTGCTGATAAAATAGAATCTGGGAAAACTTCCGTGGTCATCCACTGCAGTG
  ATGGGTGGGATCGAACATCCCAGCTCACATCTCTGGCAATGCTGATGTTAGACAGTTATTACCGGACCAT
  TAAAGGATTTGAGGCTCTCATAGAAAAGGAGTGGATAAGCTTTGGACACAGGTTTGCCCTGAGAGTGGGC
  CATGGTGATGACAACCATGCAGATGCTGACCGATCACCTATATTTCTTCAGTTTATTGATTGTGTTTGGC
  AGATGACAAGACAGTTCCCTTCAGCATTTGAGTTTAATGAATTATTCTTGATTACAATTTTGGATCACCT
  CTATAGTTGTCTTTTTGGGACCTTTTTGTGCAACTGTGAACAACAGCGAATCAAAGAGGATGTATATACA
  AATACTATATCTTTATGGTCTTATATCAATAGCCAACTAGATGAATTCTCAAATCCCTTCTTTGTGAATT
  ATGAGAACCATGTGTTATATCCTGTTGCTAGTATGAGTCACTTGGAATTGTGGGTAAATTATTATGTACG
  GTGGAATCCACGGATGAGACCACAGATGCCAATTCATCAGAATCTCAAGGAACTGCTGGCTATCAAGGCT
  GAGCTGCAGAAGCGTGTGGAGGACTTGCAGCGGGAGATGGCCACCCGGACCATCTCATCTTCCTCTGAGA
  GGGGCTCCTCACCTACCCACTCAGCTACTCCTGTACACACCTCAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209913 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRPVAAAAAASAASCEGAGGPGPGPGASWRPSRVAGGASASSRHPSIETLDSPTGSHVEWCKQLIAATI
  SSQISGSVTSENVSRDYKALRDGNKLAQMEEAPLFPGESIKAIVKDVIYICPFMGAVSGTLTVTDFKMYF
  KNVERDPHFVLDVPLGVISRVEKIGAQSHGDNSCGIEIVCKDMRNLRLAYKQEEQRKLGIFENLNKHAFP
  LSNGQVLFAFNYKEKFPVNGWKVYDPVSEYKRQGLPNESWKISKINSNYEFCDTYPAIIVVPTSVKDDDL
  SKVAAFRAKGRVPVLSWIHPESQATITRCSQPLVGPNDKRCKEDEKYLQTIMDANAQSHKLTIFDARQNS
  VADTNKAKGGGYENESAYPNAELIFLEIHNIHVMRESLRKLKEIVYPSIDESHWLSNVDGTHWLEYIRVL
  LAGAVRIADKIESGKTSVVIHCSDGWDRTSQLTSLAMLMLDSYYRTIKGFEALIEKEWISFGHRFALRVG
  HGDDNHADADRSPIFLQFIDCVWQMTRQFPSAFEFNELFLITILDHLYSCLFGTFLCNCEQQRIKEDVYT
  NTISLWSYINSQLDEFSNPFFVNYENHVLYPVASMSHLELWVNYYVRWNPRMRPQMPIHQNLKELLAIKA
  ELQKRVEDLQREMATRTISSSSERGSSPTHSATPVHTSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016985
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016985.2, NP_058681.1
• RefSeq Size: 4652 bp
• RefSeq ORF: 2010 bp
• Locus ID: 53332
• UniProt ID: Q9Z2C4
• Cytogenetics: X A7.3
• MW: 75.3 kDa
• Gene Summary: Lipid phosphatase that has high specificity for phosphatidylinositol 3-phosphate and has no activity with phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate (PubMed:12217958). Activity with phosphatidylinositol (3,5)-bisphosphate is controversial; it has been shown for the human ortholog (By similarity). In contrast, PubMed:12217958 find no activity with this substrate.[UniProtKB/Swiss-Prot Function]