Daglb (NM_144915) Mouse Tagged ORF Clone

CAT#: MR209911

Daglb (Myc-DDK-tagged) - Mouse diacylglycerol lipase, beta (Daglb)

Product Images

Circular map for MR209911

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Daglb
• Synonyms: E330036I19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209911 representing NM_144915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGGATGGTGCTGTTCGGCCGGCGCTGGTCTCTAGCCAGCGACGACTTGGTGTTCCCCGGGTCTT
  TTGAGCTGTTCCTGCGTGTGCTGTGGTGGATTGTCAGTCTGACGTTGTATCTCACGCACAGAAGGAGGCT
  GGACTGTCCCGGTGGAGTCTTGCTCAGCACGTACCTGATAGTCCTCCTTGTTCTCCTGGCAGTTATTATA
  TGCACCGTGTTGGCCATCGTGTGTGTCAGCATGAGAGGAACCATTTGTAACCCTGGACCTCGGAAGTCTA
  TGTCTAAGCTGCTCTATATCCGCCTGGCGCTCTTCCTGCCAGAGATGGTCTGGGCTTCTCTGGGGGCCGC
  CTGGGTGGCAAAAGGTATCCAGTGTGACAGGACAGTTGTCATTGGCATCATCGCCACCGTCATTGTCAGC
  TGGATTGTCATTGCTGCCACCATGGTCACCATCATCTTTGTCTTTGACCCGCTGGGCGGGAAGATGGCTC
  CGTATCCCCCATGCATCCCGGAGCACCTGGACAGTAACAGCTCAAACCGGTTACTGACTGGCCTGAAGAC
  GGCTGCGAAGAGCGTGTGGGAGACCCGGGTGCAGTTCTGCTGCTGCTGCGTTGGGCAGGATGATAACACC
  AGGGTGGCGTTTTCCAGCACTGCCGACCTGTTCTCCACCTACTTCTCTGACACAGACCTGGTGCCTAGTG
  ATATCGCAGCAGGCTTCACCCTTCTCCACCAGCAACAAGACAATATCAGCCACAGCCGGGAGCCTCCGGA
  GGTCGTGACCCACACACCAGGACAGCCTCAGGAAACTGAGTTAGATGCAGAAGTGGAGAACTGCCATCAT
  TACATGCCGTTTGCAGCAGCTGCCTATGGGTGGCCGCTCTACATCTACAGGAACCCGTTCACGGGGCTGT
  GCAGGATTGGTGGCGACTGTTGCAGAGCCAGAGACATAGAGTACGATGCAGTGGAAGGTGACCAGCACAA
  CTGCCACTTCGCCTCCATCCTGAAGACCACAGGGCTGCAGTACAGGGATTTCATTCACATAAGCTTTCAC
  GACAAGGTGTATGAGCTGCCCTTCATAGTGGTTCTGGACCACAGGAAGGAGTCTGTTGTGGTCGCCGTGA
  GAGGGACCATGTCTCTCCAGGACGTCCTGACCGACCTGTCTGCCGAGAGTGAGACCCTGGAGCTGGGGAT
  TGAGCTACAGGACTGTGTGGCCCACAAGGGAATTGCTCAAGCGGCCAGATACATTCACCGCAGACTGGTC
  AATGATGGGATTCTGAGCCAAGCCTTCAGTGTCGCTCCGGAGTACCAGCTCGTTCTGGTGGGGCACAGCC
  TAGGAGCCGGCGCTGCGGCCCTGTTAGCCATCATGCTCCGGGGGGCCTACCCACAAGTCCGTGCTTATGC
  CTTCTCCCCGCCCAGGGGGCTGCTCAGCAAATCCCTTTACGAGTACTCCAAGGACTTTGTCGTGTCGCTT
  ATCCTAGGGATGGATGTGATCCCCAGGTTAAGTGTGACCAACATGGAGGACCTCAAGAGGAGGATCCTGA
  GAGTGATCGCTAACTGCAATAAGCCGAAGTACAAGATCTTGCTGCATGGCTGTTGGTACGGACTGTTCGG
  AGGAAGCCCTGATAACTTTCCAACTGAACTGGATGAGGGCACCCAGGGGGCCCTGACTCAGCCTCTTCTT
  GGAGAGCAGACACTTCTAACCCGATACTCCCCGGGCTACTGTTCCAGCGACTCCCCACTGGACTCCCCCA
  CCAAGTACCCCACTCTGTACCCACCTGGCAGGATCATCCACCTGGAAGAGGAGGGTGGTTCAGGGAGGTT
  TGGCTGCTGTTCTGCTGCCCAGTACAGAGCGAGGTGGGCACACGAAGCAGAATTCAGTAAGATCCTGATA
  GGCCCAAAGATGCTGATTGACCACATGCCTGACGTCATGATTCGGGCCCTGGACAGAGTGCTTGCCGACA
  GGACAGCCTGCGTCTCCTGCCCAGGGCAAGGCGGCTCAAGTGTACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209911 representing NM_144915
  Red=Cloning site Green=Tags(s)
  
  MPGMVLFGRRWSLASDDLVFPGSFELFLRVLWWIVSLTLYLTHRRRLDCPGGVLLSTYLIVLLVLLAVII
  CTVLAIVCVSMRGTICNPGPRKSMSKLLYIRLALFLPEMVWASLGAAWVAKGIQCDRTVVIGIIATVIVS
  WIVIAATMVTIIFVFDPLGGKMAPYPPCIPEHLDSNSSNRLLTGLKTAAKSVWETRVQFCCCCVGQDDNT
  RVAFSSTADLFSTYFSDTDLVPSDIAAGFTLLHQQQDNISHSREPPEVVTHTPGQPQETELDAEVENCHH
  YMPFAAAAYGWPLYIYRNPFTGLCRIGGDCCRARDIEYDAVEGDQHNCHFASILKTTGLQYRDFIHISFH
  DKVYELPFIVVLDHRKESVVVAVRGTMSLQDVLTDLSAESETLELGIELQDCVAHKGIAQAARYIHRRLV
  NDGILSQAFSVAPEYQLVLVGHSLGAGAAALLAIMLRGAYPQVRAYAFSPPRGLLSKSLYEYSKDFVVSL
  ILGMDVIPRLSVTNMEDLKRRILRVIANCNKPKYKILLHGCWYGLFGGSPDNFPTELDEGTQGALTQPLL
  GEQTLLTRYSPGYCSSDSPLDSPTKYPTLYPPGRIIHLEEEGGSGRFGCCSAAQYRARWAHEAEFSKILI
  GPKMLIDHMPDVMIRALDRVLADRTACVSCPGQGGSSVP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144915
• ORF Size: 2007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144915.3, NP_659164.2
• RefSeq Size: 3257 bp
• RefSeq ORF: 2010 bp
• Locus ID: 231871
• UniProt ID: Q91WC9
• Cytogenetics: 5 G2
• MW: 74.4 kDa
• Gene Summary: Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).[UniProtKB/Swiss-Prot Function]