Cd6 (NM_009852) Mouse Tagged ORF Clone

CAT#: MR209879

Cd6 (Myc-DDK-tagged) - Mouse CD6 antigen (Cd6), transcript variant 2

Product Images

Circular map for MR209879

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209879 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCTTCCTTGGGATCGCTGGATTGCTGACAGCAGTTCTCTCAGGTCTCCCATCTCCAGCCCCCT
  CTGGCCAGCACAAAAATGGCACCATCCCGAACATGACCTTGGACCTAGAGGAGCGGCTGGGGATTCGCCT
  GGTGAACGGGAGCAGTCGCTGCAGCGGATCTGTGAAGGTTCTACTGGAGTCCTGGGAGCCTGTGTGTGCG
  GCACACTGGAACAGGGCTGCTACGGAGGCTGTGTGCAAAGCGCTGAACTGTGGTGATTCGGGAAAGGTTA
  CCTACCTGATGCCACCCACCTCTGAGCTTCCACCAGGAGCCACTTCTGGGAACACAAGCAGCGCCGGGAA
  TACGACGTGGGCGCGGGCACCAACTGAGAGATGCAGGGGAGCCAATTGGCAGTTCTGCAAGGTGCAGGAC
  CAGGAGTGTAGCAGCGACAGGCGGCTGGTGTGGGTCACCTGTGCAGAGAACCAAGCGGTGCGGTTAGTGG
  ATGGTAGCAGCCGCTGTGCTGGCCGAGTGGAGATGCTGGAGCATGGTGAATGGGGTACCGTGTGCGACGA
  CACGTGGGACCTGCAGGATGCACACGTAGTGTGTAAGCAACTGAAGTGCGGCTGGGCAGTGAAGGCGCTG
  GCCGGCTTGCACTTCACCCCAGGCCAAGGACCCATCCACCGCGATCAGGTGAACTGCTCTGGGACTGAGG
  CTTACCTGTGGGACTGCCCGGGACGGCCAGGAGACCAGTACTGTGGTCACAAGGAGGACGCAGGAGTGGT
  GTGCTCAGAGCACCAATCCTGGCGCCTGACTGGAGGCATTGACTCCTGCGAAGGGCAAGTGGAAGTGTAC
  TTCCGGGGTGTCTGGAGCACGGTGTGTGACAGCGAGTGGTACCCATCTGAGGCCAAAGTGCTCTGTCGGT
  CCTTGGGTTGCGGATCAGCAGTGGCTCGGCCCAGGGGGCTGCCACACTCCCTGGACGGTAGAATGTACTA
  CTCCTGCAAAGGACAGGAGCCCGCCCTCAGCACCTGTTCCTGGCGGTTCAACAACTCCAACCTGTGCAGC
  CAGTCGCGGGCAGCCAGGGTTGTCTGCTCAGGCTCCCAGAGACATCTCAATCTGTCTACTTCTGAAGTGC
  CTTCCAGAGTTCCAGTCACGATAGAATCTTCTGTGCCGGTGAGCGTGAAGGATAAGGACTCACAAGGGCT
  GACACTTCTCATTCTCTGTATTGTCCTGGGAATTCTCCTCCTCGTCTCCACCATCTTCATAGTTATTCTT
  CTCTTGAGAGCCAAAGGACAATACGCCCTCCCTGCTTCAGTGAACCACCAGCAACTGTCTACTGCCAACC
  AAGCAGGGATCAATAACTACCATCCTGTCCCTATCACCATTGCCAAAGAAGCTCCTATGCTGTTCATCCA
  GCCCCGGGTCCCTGCAGACTCGGACTCCAGTTCTGACTCCGACTATGAGCACTATGACTTCAGCTCCCAG
  CCGCCTGTGGCCCTGACCACCTTCTACAATTCCCAGCGACACCGGGTCACAGAGGAGGAAGCTCAGCAGA
  ACAGGTTTCAGATGCCACCCTTGGAGGAAGGACTTGAAGAGCTGCATGTTTCCCACATCCCAGCGGCTGA
  CCCCAGACCCTGTGTGGCAGATGTCCCCTCTCGGGGCTCTCAGTACCACGTGAGGAACAACAGTGATTCC
  AGCACCTCCTCTGAGGAGGGCTACTGCAATGATCCCAGCAGCAAGCCACCTCCATGGAATTCCCAGGCGT
  TTTATTCTGAGAAGAGTCCCCTCACAGAGCAGCCCCCCAACTTGGAACTGGCTGGCTCTCCGGCAGTGTT
  TTCAGGGCCCTCAGCCGATGACAGCTCCAGCACCTCATCCGGAGAGTGGTACCAGAACTTCCAGCCACCA
  CCCCAGCATCCTCCAGCAGAACAGTTTGAATGTCCAGGACCCCCTGGCCCCCAAACAGACTCCATTGATG
  AGGAGGAGGAGGATTACGATGACATCGGAGCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209879 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWLFLGIAGLLTAVLSGLPSPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCA
  AHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQD
  QECSSDRRLVWVTCAENQAVRLVDGSSRCAGRVEMLEHGEWGTVCDDTWDLQDAHVVCKQLKCGWAVKAL
  AGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGIDSCEGQVEVY
  FRGVWSTVCDSEWYPSEAKVLCRSLGCGSAVARPRGLPHSLDGRMYYSCKGQEPALSTCSWRFNNSNLCS
  QSRAARVVCSGSQRHLNLSTSEVPSRVPVTIESSVPVSVKDKDSQGLTLLILCIVLGILLLVSTIFIVIL
  LLRAKGQYALPASVNHQQLSTANQAGINNYHPVPITIAKEAPMLFIQPRVPADSDSSSDSDYEHYDFSSQ
  PPVALTTFYNSQRHRVTEEEAQQNRFQMPPLEEGLEELHVSHIPAADPRPCVADVPSRGSQYHVRNNSDS
  STSSEEGYCNDPSSKPPPWNSQAFYSEKSPLTEQPPNLELAGSPAVFSGPSADDSSSTSSGEWYQNFQPP
  PQHPPAEQFECPGPPGPQTDSIDEEEEDYDDIGAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009852
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009852.2
• RefSeq Size: 3034 bp
• RefSeq ORF: 1998 bp
• Locus ID: 12511
• UniProt ID: Q61003
• Cytogenetics: 19 7.16 cM
• MW: 72.3 kDa
• Gene Summary: Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as costimulatory molecule; promotes T-cell activation and proliferation. Contributes to the formation and maturation of the immunological synapse. Functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria. LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS.[UniProtKB/Swiss-Prot Function]