Tmco4 (NM_029857) Mouse Tagged ORF Clone

CAT#: MR209614

  • TrueORF®

Tmco4 (Myc-DDK-tagged) - Mouse transmembrane and coiled-coil domains 4 (Tmco4)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_029857" in other vectors (4)


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Reconstitution Protocol

USD 588.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tmco4
Synonyms 4632413C14Rik; AI430825
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209614 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACATGGAACAGGCCACACCCAAGGCAGCCTGTGGCACCAGAGCCAGCTGCAGAAGACGATTCCC
AGCAGCCCCTGGGCCGGGAGCTCTCGGAGGCCAATCGCTTCGCCTATGCCGCCCTGTGTGGCTTCTCGTT
GTCACAGCTGTTTCCAGAACCTGAACAAAGCTCCTTCTGCACAGAATTTGTGACGGGCCTGGTGAAATGG
CTGCATCTGTCTGAAAGTGTCCTCCCAACCATGATGGCCTTTGCCAGTGGCCTGGGAGGCGAAGGAGCCG
ACATCTTTGCTCAGACTTTATTGCAGGACCCCATTCTGAGAGACAACCCATCAGCAGTCAGTCAGGACCT
CCTGAGCTTCTCACTCAAGAATGGACACTATGATGCCCGGGCCAGAGTCCTCGTTTGTCATGTGACCTCC
CTGCTCCAGGTGCCCATGGAGGAGCTTGACATTCTGGAAGAGGTGTTCCTGGAGAGCCTGAAGGACACCA
AAGAGGAGGAGTCTGAGACCGCAGAGGCATCCCGGAAGAGGAAAGAAAAACGGAGGAAGTGGAAACGCTA
TCTTCTGATAGGCCTGGCCACAGTTGGAGGCGGGACAGTGATTGGTGTGACTGGGGGGCTAGCTGCCCCA
CTTGTCGCTGCAGGAGCCGCAACCATCATAGGCAGTGCCGGGGCAGCCGCGTTGGGCTCAGTGGCTGGCA
TCGCTGTTATGACTTCACTGTTTGGTGCAGCCGGAGCTGGCCTGACAGGATACAAGATGAAGAAGCGAGT
CGGAGCCATTGAGGAGTTCATGTTCCTGCCTTTGACGGAAGGCCGGCAGCTACATATCACCATTGCCATC
ACTGGCTGGCTCGGGTCCGGCAGATACCGCACATTTAATGCCCCATGGATGGCCCTAGCCCGAAGCCAGG
AGCAGTACTGCCTGGCCTGGGAGGCCAAGTACCTGATGGAGTTGGGCAATGCCCTGGAGACCATCCTCAG
TGGCCTCGCTAACATGGTAGCACAGGAGGCTCTCAAGTACACAGTGCTCTCTGGTATCGTGGCTGCTCTA
ACCTGGCCAGCCTCACTCCTCAGCGTCGCCAATGTCATCGACAACCCCTGGGGCGTGTGCCTCCACCGAT
CGGCAGAAGTTGGCAAGCACCTGGCGCACATCCTTCTCTCACGGCAACAGGGCCGGAGACCTGTCACCTT
GATTGGATTCAGCCTGGGAGCCAGAGTCATCTACTTCTGTCTGCAAGAAATGGCTCAGGAGCAAGATTGC
CAAGGGATCATTGAGGATGTGGTCCTTCTGGGCGCACCAGTAGAAGGGGACCCCAAGCACTGGGAACCAT
TCCGGAATGTGGTGTCTGGGAGAATCATCAATGGCTACTGCAGGGGAGACTGGCTGCTGAGCTTCGTGTA
CCGCACATCTTCGGTGCAGCTCCGCGTCGCCGGCCTCCAGCCTGTGCTGCTGCAGGACCGGAGGATGGAG
AATGTGGACCTGACCTCCGTGGTCAATGGCCACTTGGACTACGCCAAGCAAATGGATGCCATCCTGAAGG
TTGTGGGCATCCGCACCAAGCCTGGCTGGGGAGAGAAGGGACTCCCACTGGCCCCTGGCAGCCTGCCCCA
AGAAGAGCCTCTCCAGACAGCCATAGTCTCTACAGATGAGACAATCCTCCAGGATGGACAAAGCCAGGGA
CCTGCCTCAGAAGACAGTCTCAAAACCACCATACCTTCCAGTGCCAGCCAAGCCCAGATGCCAGCAGGGC
TGAACCAGTCCACAGAGGACTCGCTCTCTACTGCTGCTGCTCCGGCAGAGGGACACCTGATCTGCAGCCA
TGGTGTGGGTCCCAACCCACTGGGCTGCCCTGACTGCACCCATGGGACCCAGGAGTCCTGTACAGAACTG
GAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209614 protein sequence
Red=Cloning site Green=Tags(s)

MATWNRPHPRQPVAPEPAAEDDSQQPLGRELSEANRFAYAALCGFSLSQLFPEPEQSSFCTEFVTGLVKW
LHLSESVLPTMMAFASGLGGEGADIFAQTLLQDPILRDNPSAVSQDLLSFSLKNGHYDARARVLVCHVTS
LLQVPMEELDILEEVFLESLKDTKEEESETAEASRKRKEKRRKWKRYLLIGLATVGGGTVIGVTGGLAAP
LVAAGAATIIGSAGAAALGSVAGIAVMTSLFGAAGAGLTGYKMKKRVGAIEEFMFLPLTEGRQLHITIAI
TGWLGSGRYRTFNAPWMALARSQEQYCLAWEAKYLMELGNALETILSGLANMVAQEALKYTVLSGIVAAL
TWPASLLSVANVIDNPWGVCLHRSAEVGKHLAHILLSRQQGRRPVTLIGFSLGARVIYFCLQEMAQEQDC
QGIIEDVVLLGAPVEGDPKHWEPFRNVVSGRIINGYCRGDWLLSFVYRTSSVQLRVAGLQPVLLQDRRME
NVDLTSVVNGHLDYAKQMDAILKVVGIRTKPGWGEKGLPLAPGSLPQEEPLQTAIVSTDETILQDGQSQG
PASEDSLKTTIPSSASQAQMPAGLNQSTEDSLSTAAAPAEGHLICSHGVGPNPLGCPDCTHGTQESCTEL
D

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_029857
ORF Size 1896 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_029857.1, NM_029857.2, NM_029857.3, NP_084133.1
RefSeq Size 3322 bp
RefSeq ORF 1896 bp
Locus ID 77056
UniProt ID Q91WU4
Cytogenetics 4 D3
MW 67.9 kDa

Other Versions

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