Actr8 (NM_027493) Mouse Tagged ORF Clone

CAT#: MR209526

Actr8 (Myc-DDK-tagged) - Mouse ARP8 actin-related protein 8 homolog (S. cerevisiae) (Actr8)

Product Images

Circular map for MR209526

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Actr8
• Synonyms: 5730542K05Rik; ARP8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209526 representing NM_027493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAAGCCGAGAAGGGGGACGCGGAGAACGGGAAGGAGAAGGGCGGGGAGAAGGAGAAGGAGCAGC
  GCGGCGTGAAGCGGCCCATCGTACCCGCGTTGGTCCCCGAGTCGCTGCAGGAGCAAATCCAGAGCAACTT
  CATTGTTGTCATACACCCAGGTTCAACAACTCTAAGACTTGGCCGAGCCACAGATACACTTCCGGTCAGT
  GTTCCTCACGTCATTGCACGAAGACACAAACAACAAGGGCAGCCCCTGTACAAGGACAACTGGCTCCTAA
  GGGAAGGACTAAATAAACCTGAAAGTAATGAACAAAGACAAAATGGCCTTAAAATGGTGGACCAGGCAAT
  ATGGTCTAAAAAGATGTCAAACGGTACAAGGCGGATCCCTGTGTCACCAGAACAGACACGCTCCTACAAC
  AAGCAGATGCGACCTGCCATTCTAGATCACTGTTCTGGAAACAAATGGACAAACACATCTCAGCAGCCTG
  AGTATTTGGTAGGAGAAGAGGCCTTGTATGTTAACCCCCTGGACTGTTACAACATTCACTGGCCTATCAG
  AAGGGGTCAGCTGAACATCCACCCAGGCCCCGGAGGCTCCCTCACAGCTGTTCTGGCAGACATTGAAGTG
  ATCTGGTCTCATGCAATACAAAAGTATTTGGAAATCCCACTGAAAGATTTAAAGTATTACAGGTGCATCT
  TGTTAATTCCAGATATCTATAACAAGCAGCATGTGAAAGAGCTGGTCCATATGATACTGATGAAGATGGG
  CTTCGCAGGGATTGTGGTCCATCAGGAGTCTGTGTGCGCTACGTTTGGGAGCGGCTTGAGCAGCACCTGT
  GTTGTAGATGTTGGGGACCAGAAAACAAGTGTGTGCTGTGTAGAGGATGGGGTGTCTCATCGGAACACAC
  GGCTTTGTCTGGCCTATGGGGGCTCTGACGTGTCGAGGTGTTTTTACTGGCTCATGCAGCGAGCGGGATT
  CCCTTACAGAGAGTGTCAGCTGACAAATAAAATGGATTGCCTCCTCTTGCAGCACCTTAAAGAAACCTTT
  TGTCATTTAGATCAGGATATCTCCGGACTTCAGGACCATGAGTTTCAGATTCGGCATCCGGATTCCCCTG
  CCCTGCTTTACCAGTTTCGATTAGGAGATGAAAAGCTCCAGGCTCCAATGGCGCTTTTTTACCCAGCAAC
  CTTTGGGATCGTGGGACAGAAGATGACCACTCTGCAGCACAGGTCCCAGGGAGACCCTGAGGATCCCCAT
  GATGAGCACTACCTGCTGGCCACCCAGAGCAAGCAAGAACAGTCTGCAAAAGCCACTGCTGACCGCAAGT
  CTGCATCCAAACCCATTGGATTTGAAGGGGATCTGCGTGGCCAGTCCTCTGATCTTCCAGAAAGACTCCA
  TTCCCAGGAGGTGGACCTGGCTTCTTCTCAGGGAGACTGTTTGATGGCGGGCAATGAGTCCGAGGAAGCC
  CTCACTGCACTGATGTCCAGGAAAACTGCAATCTCGCTGTTTGAGGGGAAAGCCTTGGGCCTTGATAAGG
  CCATCCTGCACAGCGTAGACTGTTGCTCATCTGATGATACCAAAAAGAAGATGTATAGCTCTATCCTGGT
  TGTGGGAGGCGGTTTGATGTTTCATAAGGCTCAAGAGTTTCTCCAGCACAGAATTCTCAACAAAATGCCG
  CCTTCATTCAGGCGAATTATTGAAAACGTGGATGTGATCACAAGGCCCAAGGACATGGACCCCCGGCTGA
  TCGCATGGAAAGGAGGTGCAGTACTGGCGTGTTTGGACACCACTCAGGAACTGTGGATTTATCAGCGAGA
  GTGGCAACGCTTTGGTGTCCGAATGTTACGGGAACGAGCTGCCTTTGTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209526 representing NM_027493
  Red=Cloning site Green=Tags(s)
  
  MTQAEKGDAENGKEKGGEKEKEQRGVKRPIVPALVPESLQEQIQSNFIVVIHPGSTTLRLGRATDTLPVS
  VPHVIARRHKQQGQPLYKDNWLLREGLNKPESNEQRQNGLKMVDQAIWSKKMSNGTRRIPVSPEQTRSYN
  KQMRPAILDHCSGNKWTNTSQQPEYLVGEEALYVNPLDCYNIHWPIRRGQLNIHPGPGGSLTAVLADIEV
  IWSHAIQKYLEIPLKDLKYYRCILLIPDIYNKQHVKELVHMILMKMGFAGIVVHQESVCATFGSGLSSTC
  VVDVGDQKTSVCCVEDGVSHRNTRLCLAYGGSDVSRCFYWLMQRAGFPYRECQLTNKMDCLLLQHLKETF
  CHLDQDISGLQDHEFQIRHPDSPALLYQFRLGDEKLQAPMALFYPATFGIVGQKMTTLQHRSQGDPEDPH
  DEHYLLATQSKQEQSAKATADRKSASKPIGFEGDLRGQSSDLPERLHSQEVDLASSQGDCLMAGNESEEA
  LTALMSRKTAISLFEGKALGLDKAILHSVDCCSSDDTKKKMYSSILVVGGGLMFHKAQEFLQHRILNKMP
  PSFRRIIENVDVITRPKDMDPRLIAWKGGAVLACLDTTQELWIYQREWQRFGVRMLRERAAFVW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027493
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027493.3, NP_081769.2
• RefSeq Size: 2121 bp
• RefSeq ORF: 1875 bp
• Locus ID: 56249
• UniProt ID: Q8R2S9
• Cytogenetics: 14 A3
• MW: 70.5 kDa
• Gene Summary: Plays an important role in the functional organization of mitotic chromosomes. Exhibits low basal ATPase activity, and unable to polymerize (By similarity).[UniProtKB/Swiss-Prot Function]