Ctif (NM_201354) Mouse Tagged ORF Clone

CAT#: MR209515

Ctif (Myc-DDK-tagged) - Mouse CBP80/20-dependent translation initiation factor (Ctif)

Product Images

Circular map for MR209515

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctif
• Synonyms: Gm672
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209515 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACTCCTCAGCAGCTTCAGCCTCCTCTGAGGCAGGAAGCAGCCGCTCCCAGGAGATCGAGGAGC
  TGGAGCGCTTCATTGACAGCTACGTGCTGGAGTACCAGGTGCAGGGGCTGCTGACCGACAAGACAGAGGG
  TGACGGCGAGAGCCAGAGGACACAGTCCCATATCTCCCAGTGGACGGCTGACTGCAGAGAGCAGCTGGAT
  GGTAGCTGTTCCTTCTCCAGAGGGCGAGCCCCGCCACAGCAGAATGGCAACAAGGACAACTCCCTGGACA
  TGCTGGGCACAGATATCTGGGCCGCCAATACCTTTGATTCCTTCAGTGGTGCCACCTGGGACCTGCAGCC
  TGAAAAGCTGGACTTCACCCAGTTCCATCGGAAGGTCCGCCACACACCCAAGCAGCCCCTGCCACACATC
  GACAGAGAAGGGTGTGGAAAGGGAAAGCTGGAAGATGGAGATGGTATCAGCCTGAATGACATTGAGAAGG
  TCCTCCCGACCTGGCAGGGCTACCATCCGATGCCCCACGAAGCAGAGATTGCACATACCAAGAAGTTGTT
  CCGAAGGAGGAGAAATGACCGAAGACGGCAGCAAAGACCTCCAGGAGGGAACAAGCCCCAACAGCATGGT
  GACCATCAGCCAGGCAGTGCCAAACACAACAGGGACCACCAGAAATCTTACCAGGGGGGCTCAGGGCCCC
  ACCCCTCAGGGAGGCCCACACACCATGGCTACAGCCAGAACCGACGCTGGCATCACGGCAACATGAAACA
  TCCACCGGGAGACAAGGGAGAAGCGGGCAGCCACCGCAACGCCAAAGAGACCGTGACCGTTGAGAACCCC
  AAACTTGAAGACGGCCCAGGGGACACCGGACACAGTGGCCTTGAGCCCCCCTGCAGCCCTGACACTCTGA
  CCCCAGCAGCTTCCGAGAGACCAACCCCTCAGCTGCCAGGGGGGCCGGAGGCTGAGATAAAGCATAAAGA
  CACTGTTCTTCCTGAGCGTCTCAGGGAGCGGCCCAAAATCACCCTGCTCCAGTCTTCCAAAGACAGGCTG
  AGGCGAAGGCTGAAGGAAAAGGACCGGGACATCCCGAATCCCACTGAGACTTCAGCTCCACTGAGGTGTG
  TTTTATGTGTCCCCCATGTCCCCCAGGACGAAGTGGCAGTAGAGACAAGCAGCCCTCAGCCAAGCAAGAT
  GGACAGGCTGATGGAAATCCTTAACATCATGAGAAATAACAGCAGCGACGTGGATGCCAAGCTCACCTCC
  TTCATGGAGGAGGCGCAGAACTCCACCAACTCTGAGGAGATGCTGGGGGAGATCGTGCGGACCATCTACC
  AGAAGGCCGTGTCAGACCGCAGCTTCGCCTTCACGGCCGCCAAGCTCTGCGACAAGATGGCCCTCTTCAT
  GGTCGAGGGGACCAAGTTCCGGAGCCTGCTCCTCAACATGCTCCAGAAGGACTTCACTGTGCGGGAGGAG
  CTGCAGCAACAGGATGTGGAGCGCTGGCTGGGCTTCATCACCTTCCTGTGTGAGGTGTTCGGCACCATGC
  GGAGCAGCACTGGCGAGCCCTTCCGAGTGCTGGTGTGCCCCATCTACACGTGCCTCCGGGAGCTCTTGCA
  GTCACAGGATGTGAAGGAAGACGCAGTCCTCTGCTGTTCTATGGAGCTACAAAGCACAGGCCGGCTGCTA
  GAGGAGCAGCTGCCAGAGATGATGACGGAGCTCCTAGCCAGCGCAAGAGATAAGATGCTGTGCCCCTCAG
  AGTCCATGCTAACCCGGTCCCTGCTCCTGGAGGTCATCGAGCTCCACGCCAACAGCTGGAACCCTCTGAC
  GCCCCCCATCACGCAGTACTACAACAGGACCATCCAGAAACTGACAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209515 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENSSAASASSEAGSSRSQEIEELERFIDSYVLEYQVQGLLTDKTEGDGESQRTQSHISQWTADCREQLD
  GSCSFSRGRAPPQQNGNKDNSLDMLGTDIWAANTFDSFSGATWDLQPEKLDFTQFHRKVRHTPKQPLPHI
  DREGCGKGKLEDGDGISLNDIEKVLPTWQGYHPMPHEAEIAHTKKLFRRRRNDRRRQQRPPGGNKPQQHG
  DHQPGSAKHNRDHQKSYQGGSGPHPSGRPTHHGYSQNRRWHHGNMKHPPGDKGEAGSHRNAKETVTVENP
  KLEDGPGDTGHSGLEPPCSPDTLTPAASERPTPQLPGGPEAEIKHKDTVLPERLRERPKITLLQSSKDRL
  RRRLKEKDRDIPNPTETSAPLRCVLCVPHVPQDEVAVETSSPQPSKMDRLMEILNIMRNNSSDVDAKLTS
  FMEEAQNSTNSEEMLGEIVRTIYQKAVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREE
  LQQQDVERWLGFITFLCEVFGTMRSSTGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLL
  EEQLPEMMTELLASARDKMLCPSESMLTRSLLLEVIELHANSWNPLTPPITQYYNRTIQKLTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201354
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201354.1, NP_958742.1
• RefSeq Size: 6192 bp
• RefSeq ORF: 1872 bp
• Locus ID: 269037
• UniProt ID: Q6PEE2
• Cytogenetics: 18 E3
• MW: 70.3 kDa
• Gene Summary: Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD) (By similarity).[UniProtKB/Swiss-Prot Function]