Ctif (NM_201354) Mouse Untagged Clone

CAT#: MC202400

Ctif (untagged) - Mouse CBP80/20-dependent translation initiation factor (Ctif), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctif
• Synonyms: Gm672
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058104 sequence for NM_201354
  CGACTGGGTCGGTCCGCTGAGGTGCCGGATCCCCCTCACGGCTGGGACGCGCCGAACTCCCAGCAGGAGT CCGCTCTTTGCAGCCTGGTCCCTCCCTTCCCCTTCCCTGCCCCCTTCCCCCACCCCGACGCGGGCTCCGC GCGGCAGCCAGAGGAACCCCGAGCCGGCCCCGGTCCCTGAGCCCGAGGAATGGAGAACTCCTCAGCAGCT TCAGCCTCCTCTGAGGCAGGAAGCAGCCGCTCCCAGGAGATCGAGGAGCTGGAGCGCTTCATTGACAGCT ACGTGCTGGAGTACCAGGTGCAGGGGCTGCTGACCGACAAGACAGAGGGTGACGGCGAGAGCCAGAGGAC ACAGTCCCATATCTCCCAGTGGACGGCTGACTGCAGAGAGCAGCTGGATGGTAGCTGTTCCTTCTCCAGA GGGCGAGCCCCGCCACAGCAGAATGGCAACAAGGACAACTCCCTGGACATGCTGGGCACAGATATCTGGG CCGCCAATACCTTTGATTCCTTCAGTGGTGCCACCTGGGACCTGCAGCCTGAAAAGCTGGACTTCACCCA GTTCCATCGGAAGGTCCGCCACACACCCAAGCAGCCCCTGCCACACATCGACAGAGAAGGGTGTGGAAAG GGAAAGCTGGAAGATGGAGATGGTATCAGCCTGAATGACATTGAGAAGGTCCTCCCGACCTGGCAGGGCT ACCATCCGATGCCCCACGAAGCAGAGATTGCACATACCAAGAAGTTGTTCCGAAGGAGGAGAAATGACCG AAGACGGCAGCAAAGACCTCCAGGAGGGAACAAGCCCCAACAGCATGGTGACCATCAGCCAGGCAGTGCC AAACACAACAGGGACCACCAGAAATCTTACCAGGGGGGCTCAGGGCCCCACCCCTCAGGGAGGCCCACAC ACCATGGCTACAGCCAGAACCGACGCTGGCATCACGGCAACATGAAACATCCACCGGGAGACAAGGGAGA AGCGGGCAGCCACCGCAACGCCAAAGAGACCGTGACCGTTGAGAACCCCAAACTTGAAGACGGCCCAGGG GACACCGGACACAGTGGCCTTGAGCCCCCCTGCAGCCCTGACACTCTGACCCCAGCAGCTTCCGAGAGAC CAACCCCTCAGCTGCCAGGGGGGCCGGAGGCTGAGATAAAGCATAAAGACACTGTTCTTCCTGAGCGTCT CAGGGAGCGGCCCAAAATCACCCTGCTCCAGTCTTCCAAAGACAGGCTGAGGCGAAGGCTGAAGGAAAAG GACCGGGACATCCCGAATCCCACTGAGACTTCAGCTCCACTGAGGTGTGTTTTATGTGTCCCCCATGTCC CCCAGGACGAAGTGGCAGTAGAGACAAGCAGCCCTCAGCCAAGCAAGATGGACAGGCTGATGGAAATCCT TAACATCATGAGAAATAACAGCAGCGACGTGGATGCCAAGCTCACCTCCTTCATGGAGGAGGCGCAGAAC TCCACCAACTCTGAGGAGATGCTGGGGGAGATCGTGCGGACCATCTACCAGAAGGCCGTGTCAGACCGCA GCTTCGCCTTCACGGCCGCCAAGCTCTGCGACAAGATGGCCCTCTTCATGGTCGAGGGGACCAAGTTCCG GAGCCTGCTCCTCAACATGCTCCAGAAGGACTTCACTGTGCGGGAGGAGCTGCAGCAACAGGATGTGGAG CGCTGGCTGGGCTTCATCACCTTCCTGTGTGAGGTGTTCGGCACCATGCGGAGCAGCACTGGCGAGCCCT TCCGAGTGCTGGTGTGCCCCATCTACACGTGCCTCCGGGAGCTCTTGCAGTCACAGGATGTGAAGGAAGA CGCAGTCCTCTGCTGTTCTATGGAGCTACAAAGCACAGGCCGGCTGCTAGAGGAGCAGCTGCCAGAGATG ATGACGGAGCTCCTAGCCAGCGCAAGAGATAAGATGCTGTGCCCCTCAGAGTCCATGCTAACCCGGTCCC TGCTCCTGGAGGTCATCGAGCTCCACGCCAACAGCTGGAACCCTCTGACGCCCCCCATCACGCAGTACTA CAACAGGACCATCCAGAAACTGACAGCCTGACAGCCCAGCCGAGGGGGCCTGGCAGGCAGCCCGTGGGCA GCTGGGGCCCTAGTGCACAGGGCCAGATGGACAGGCGCAAGGACAGGGGTGGCCCTGGCGGGAAAGAGAA CTGAGCAGGGAGGCAGGTAGCCACGGTGGCCGGTCTGGAGCCAGCGGAGAAGGAGGAGATTGGATCCCTC AGAAGGGCTCCCCGTGCAGCCCCACCCCCACCCACCCCCAAGCCTGCGCATGTGGCAGCTCACACCAAGG GAAAGCATGTCTCTTTGTCCTGGTGGTCACCCGTCCTTGCTCTCTTCTCCTCGGCTCCAGTCCTCCCCAC CCCCTACCTTGCAGCCCATTCCCCCCTCGGCCGCCCCCCCCCTCCCCCATAGAGCTTTATGGGAGGAGTC TCTGCCTCTCCTCACTGTCTACAGCTGCCTCTCTGCCTCCCACACCTCTGGCCACCGCTTGGGTCAGCCA GGACTGCTGAAGGCTGAAAAAGTGGGTCGAGACAGCTCTCATTGTTCCCACGTGCTGTCAGCCACGGTCG CCAACTGGCAGCAGGCAGCGGGCAGCAGATGTCTGGGAGGACAAAGGCAGGCACGGTCCCCACCAGCCGC CCATAATTGATGGCCTTTGTCAGCCCCAGCGGAGCTGGAGAGAGGCTTTTTTTTCTCCTCTAACCTCCGT GCTCCACCCTCCAACCCTCGGCCTCCAGCCCCAGGGCCCACTGCTCCCCCTGGCAGAGAAGGGAGAGCCA GGGCTCACAGCTCAGCGTGAACCCTTTTATTTTGTTTTTCAAAACTCAGTGGCTTTGAAGTCTCTTTTCC TCAATGAAAATGCCCTAGATTTTGAGATCACATCGTTCTCCCCTCAAGGATCCCCTGATTAGTGGGAGAC TAAGACCTGCCCCCCCTCCCCAAAGAATCTAGCCACTAGCAGCTTGCCAGTAGCCAGCTAGTAATTGGAA ACTTCTGCTCCAATGGGGGTCCCCACTGGAATCCTGTGTTCCTGGGCCCCTGTCCTGCAGTGCCCCTGTT CTGTCCACTGCTAGCTTCCAAAGGGCTGGACTGACTGCCTTAGGACCTGCCCTGGCTCCTGTTACTGGCA AACGGGGCCAGAAACGAGGCTGGCAAATTACAACCAAATGCAGGTCTTGTTCCGAGCAACTGTTGAGGGA AGAGCCCACTGCTGACTCCACAGATCTTGGAGCCTCCTTCATGATCCCATGCCGCATCCCAGGCAAGACC CACAAGCCCTACCTCCAAGGCTGCTGGGGAGAGGGGGAATCATTGGGTCCTGACTGGGACGGTTTGGTAG GCAGCCAGATAGACAATGAAGTTCTTCAGGCTAGAAACAAAGTCCTGGTTTCTTGGGAGGCTCTGGTCCC AGGGAGCAACCACTTCTGAATGAACAGGAGGCAAAGCCTCTCTCAAACTTGTGTTGTCCTGTCTGGCCAT GATGCTCTGATATTGAGCCTTTTTAGGCTCCGCCCACCTGTCAGGACAAGTTGGTCCTTGTTTTCCACTC AAGTGGCCTGCTGGTCACTGCCCTCCTCATGAACTGGCCGTATTGCCTAAGCTCTGGGACCACAGCTCCT GTCGGGCTCTTGAACTCCCCTGGACAACCAACCCCTTCCACCAACCATGGGGAACCTCTTCCCCCACCCC CAAAAAAAACCATCTTCACCTGGGCATCGTTCCTCATCCAGTCTGTAAACCTTTACAGTTTGCGGATCAT CTTGTCCATTTGTGTACATGTAAATGTTGACCCAATTGGTATTTATTCTAATTTTTATTGTATTTTATTT TATTCTGTCTGAGGGTGGGGGATGGGAAGTACACCACTGGTGGCAACCAGGGCAGAGCTGCAGGGATGCA GCCCAGGAAGCAAGAGAAGCACCTCCCTGGGGCCACTCTAGATGGCAGCCTGTCAGTACCCACACCCTGG GCTGTTATCTGGAATTGGAAGCCGGACAGTGTTCGTCATCCCATACACACAGGCCTGCCCGTGGCATGAT GTCCCTCAGCTGCCTGGCACCATGTTGGGAAACCTCTGACTGGCTCTTCGAGGAGGTGGGTTGGAGAGAC TAGAATTCTCCTCTGCTCAGGGCACTGGCCCCAGAAGATCTATCTGGAGCCTCACTGAGCCAGCTGGGAG CCTGACAACAGGACCTACCTCCCTGGAGCAGGGTCTGGAGATTGCCGCCAAAGCTATGGGCCAACCCTGC TCGCGCCGACCCCTTCCATCTACCCGTATATCCTGCTTCCCAGCTGAACCCAAACTACAAGTGGGTTTAA AAAAAATAAATAAACGATACCAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_201354
• Insert Size: 1872 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058104, AAH58104
• RefSeq Size: 4380 bp
• RefSeq ORF: 1872 bp
• Locus ID: 269037
• UniProt ID: Q6PEE2
• Cytogenetics: 18 E3
• Gene Summary: Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD) (By similarity).[UniProtKB/Swiss-Prot Function]